Invitrogen

CD105 (Endoglin) Monoclonal Antibody (SN6), Functional Grade, eBioscience™

Product Image Gallery

CD105 (Endoglin) Antibody in Immunocytochemistry (ICC/IF) — Immunostaining of human FF cells for specific cell surface markers after 48h. (a) Red arrows show cells low positive expression of CD105, (c) negative expression of CD34. Cell nuclei were counterstained by DAPI (b, d); 20X.

CD105 (Endoglin) Antibody in Immunohistochemistry (IHC) — FIG. 4. Cluster of differentiation 34 (CD34) costains with CD105. Maximum intensity projection images of mouse inguinal fat pad, human abdominal fat, and human gluteofemoral adipose tissue stained with CD34, Hoescht, and the ASC marker CD105. Merged images demonstrate a high degree of overlap between CD34 and CD105 antibody binding. ASC, adipose-derived stromal cells.

CD105 (Endoglin) Antibody in Western Blot (WB) — Figure 7 ENG affects VEGFR2 trafficking and downstream signalling. ( a ) Immunoprecipitation of VEGFR2 with protein samples from VEGF-treated HDMECs lenti-virally transduced with either control shRNA or shRNA targeting ENG. Phosphorylated VEGFR2 was detected by blotting with pTyr antibody. ( b ) Ratio of Phosphorylated/total VEGFR2 was measured by ImageJ, n=4 independent experiments. ( c, d ) Western blot of total cell lysates of HDMECs, indicates knockdown of ENG in the sh ENG condition (non-reduced condition). VEGFA treatment for indicated time points reveals differential loss of VEGFR2 over time in control versus knockdown cells as shown by densitometric analysis relative to the loading control calnexin. P <0.001, 2-way ANOVA, n=3 independent experiments. ( e ) Total cell lysates of the above analysed for VEGFR2 downstream components p*AKT, pan AKT, p*ERK1/2 and the loading control calnexin. ( f, g ) Densitometric quantification of p*AKT/total AKT or p*ERK1/2 /total ERK showing potentiated AKT phosphorylation in ENG knockdown ECs ( P <0.001, 2-way ANOVA, n=7 independent experiments for time points 0, 10, 30 min; n=3 independent experiments for time points 5, 15, 20 min) and no difference in p* ERK1/2. ( h ) Cellular membrane proteins were isolated at different time points and analysed for VEGFR2 (note the absence of the intracellular unglycosylated band), ENG, p*Y and total amount (biotin). ( i ) Densitometric analysis of membrane localised VEGFR2 in ( h ). Red or green do

CD105 (Endoglin) Antibody in Flow Cytometry (Flow) — Figure S2. Characterization of hiPSC-derived mesenchymal stem cells (MSCs). (A) The percentage of MSCs was determined at different time points by flow cytometry by detection of CD73 (ecto 5' nucleotidase), CD90 (Thy1), CD105 (Endoglin). (B) Representative flow cytometry analysis across samples to validate the absence of hematopoietic cell lineage markers CD34 and CD45. (C) After the first differentiation step, cells at 80% confluence were divided and expanded. The time between each passage is indicated. Cells were collected at P7, 40 d after differentiation for Hutchinson-Gilford progeria syndrome cells and 47 d for cells from the aged healthy donor. (D) The percentage of proliferating cells was monitored by flow cytometry at each passage using Ki67 staining. We observed a similar proliferation rate between the different conditions. Between each passage, measurements were made at three time points: 12, 24 and 48 h.

Product Details

16-1057-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

View 54 publications 54 publications

Functional Assay (FN)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Cat, Fruit fly, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SN6

Conjugate

Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

RRID

AB_529467

Product Specific Information

Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin. CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.

Applications Reported: This SN6 antibody has been reported to have functional activity.

Applications Tested: This SN6 antibody has been tested by flow cytometric analysis of U937 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mesenchymal stem cells derived from patients with premature aging syndromes display hallmarks of physiological aging.

Life science alliance

Trani JP,Chevalier R,Caron L,El Yazidi C,Broucqsault N,Toury L,Thomas M,Annab K,Binetruy B,De Sandre-Giovannoli A,Levy N,Magdinier F,Robin JD

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Sep 14 00:00:00 EDT 2022

Aberrant Expression of COX-2 and FOXG1 in Infrapatellar Fat Pad-Derived ASCs from Pre-Diabetic Donors.

Cells

O'Donnell BT,Monjure TA,Al-Ghadban S,Ives CJ,L'Ecuyer MP,Rhee C,Romero-Lopez M,Li Z,Goodman SB,Lin H,Tuan RS,Bunnell BA

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Human Amniotic Mesenchymal Stem Cells Inhibit aGVHD by Regulating Balance of Treg and T Effector Cells.

Journal of inflammation research

Gao Y,Li W,Bu X,Xu Y,Cai S,Zhong J,Du M,Sun H,Huang L,He Y,Hu X,Liu Q,Jin H,Wang Q,Ping B

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Tue Apr 26 00:00:00 EDT 2022

The Effect of Different Doses of Melatonin on in Vitro Maturation of Human Follicular Fluid-Derived Oocyte-Like Cells.

JBRA assisted reproduction

Azandeh S,Moghadam MT,Rashno M,Zargar M,Zadeh PA

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Immunocytochemistry

Sun Apr 17 00:00:00 EDT 2022

Exosomes derived from stem cells of human deciduous exfoliated teeth inhibit angiogenesis in vivo and in vitro via the transfer of miR-100-5p and miR-1246.

Stem cell research & therapy

Liu P,Zhang Q,Mi J,Wang S,Xu Q,Zhuang D,Chen W,Liu C,Zhang L,Guo J,Wu X

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu Mar 03 00:00:00 EST 2022

Induced Neurodifferentiation of hBM-MSCs through Activation of the ERK/CREB Pathway via Pulsed Electromagnetic Fields and Physical Stimulation Promotes Neurogenesis in Cerebral Ischemic Models.

International journal of molecular sciences

Park HJ,Choi JH,Nam MH,Seo YK

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Fri Jan 21 00:00:00 EST 2022

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Scientific reports

Chen C,Huang X,Zhu W,Ding C,Huang P,Li R

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model.

Stem cell research & therapy

Li Y,Shi G,Han Y,Shang H,Li H,Liang W,Zhao W,Bai L,Qin C

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu Jul 15 00:00:00 EDT 2021

Development, characterization, and hematopoietic differentiation of Griscelli syndrome type 2 induced pluripotent stem cells.

Stem cell research & therapy

Güney-Esken G,Erol ÖD,Pervin B,Gürhan Sevinç G,Önder T,Bilgiç E,Korkusuz P,Günel-Özcan A,Uçkan-Çetinkaya D,Aerts-Kaya F

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Therapeutic Effects of Human Urine-Derived Stem Cells in a Rat Model of Cisplatin-Induced Acute Kidney Injury In Vivo and In Vitro.

Stem cells international

Sun B,Luo X,Yang C,Liu P,Yang Y,Dong X,Yang Z,Xu J,Zhang Y,Li L

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Fri Nov 27 00:00:00 EST 2020

The Effect of Angiotensin II, Retinoic Acid, EGCG, and Vitamin C on the Cardiomyogenic Differentiation Induction of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.

International journal of molecular sciences

Gasiūnienė M,Valatkaitė E,Navakauskaitė A,Navakauskienė R

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu Nov 19 00:00:00 EST 2020

Vasculogenesis from Human Dental Pulp Stem Cells Grown in Matrigel with Fully Defined Serum-Free Culture Media.

Biomedicines

Luzuriaga J,Irurzun J,Irastorza I,Unda F,Ibarretxe G,Pineda JR

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Mon Nov 09 00:00:00 EST 2020

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

ATF6 safeguards organelle homeostasis and cellular aging in human mesenchymal stem cells.

Cell discovery

Wang S,Hu B,Ding Z,Dang Y,Wu J,Li D,Liu X,Xiao B,Zhang W,Ren R,Lei J,Hu H,Chen C,Chan P,Li D,Qu J,Tang F,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

ZKSCAN3 counteracts cellular senescence by stabilizing heterochromatin.

Nucleic acids research

Hu H,Ji Q,Song M,Ren J,Liu Z,Wang Z,Liu X,Yan K,Hu J,Jing Y,Wang S,Zhang W,Liu GH,Qu J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2020

Resveratrol rescues TNF‑α‑induced inhibition of osteogenesis in human periodontal ligament stem cells via the ERK1/2 pathway.

Molecular medicine reports

Yuan J,Wang X,Ma D,Gao H,Zheng D,Zhang J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

Chondrogenic Differentiation from Induced Pluripotent Stem Cells Using Non-Viral Minicircle Vectors.

Cells

Rim YA,Nam Y,Park N,Jung H,Lee K,Lee J,Ju JH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction.

Protein & cell

Wang S,Min Z,Ji Q,Geng L,Su Y,Liu Z,Hu H,Wang L,Zhang W,Suzuiki K,Huang Y,Zhang P,Tang TS,Qu J,Yu Y,Liu GH,Qiao J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer.

Cancers

Huang JY,Wang YY,Lo S,Tseng LM,Chen DR,Wu YC,Hou MF,Yuan SF

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Fri Dec 20 00:00:00 EST 2019

TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.

Molecular medicine reports

Fan C,Ji Q,Zhang C,Xu S,Sun H,Li Z

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Telomere-dependent and telomere-independent roles of RAP1 in regulating human stem cell homeostasis.

Protein & cell

Zhang X,Liu Z,Liu X,Wang S,Zhang Y,He X,Sun S,Ma S,Shyh-Chang N,Liu F,Wang Q,Wang X,Liu L,Zhang W,Song M,Liu GH,Qu J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Stabilizing heterochromatin by DGCR8 alleviates senescence and osteoarthritis.

Nature communications

Deng L,Ren R,Liu Z,Song M,Li J,Wu Z,Ren X,Fu L,Li W,Zhang W,Guillen P,Izpisua Belmonte JC,Chan P,Qu J,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Fri Jul 26 00:00:00 EDT 2019

Chemical screen identifies a geroprotective role of quercetin in premature aging.

Protein & cell

Geng L,Liu Z,Zhang W,Li W,Wu Z,Wang W,Ren R,Su Y,Wang P,Sun L,Ju Z,Chan P,Song M,Qu J,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Up-regulation of FOXD1 by YAP alleviates senescence and osteoarthritis.

PLoS biology

Fu L,Hu Y,Song M,Liu Z,Zhang W,Yu FX,Wu J,Wang S,Izpisua Belmonte JC,Chan P,Qu J,Tang F,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

Utilizing Confocal Microscopy to Characterize Human and Mouse Adipose Tissue.

Tissue engineering. Part C, Methods

Blackshear CP,Borrelli MR,Shen EZ,Ransom RC,Chung NN,Vistnes SM,Irizarry D,Nazerali R,Momeni A,Longaker MT,Wan DC

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Immunocytochemistry

Mon Oct 01 00:00:00 EDT 2018

Utilizing Confocal Microscopy to Characterize Human and Mouse Adipose Tissue.

Tissue engineering. Part C, Methods

Blackshear CP,Borrelli MR,Shen EZ,Ransom RC,Chung NN,Vistnes SM,Irizarry D,Nazerali R,Momeni A,Longaker MT,Wan DC

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Immunocytochemistry

Mon Oct 01 00:00:00 EDT 2018

Antagonizing CD105 enhances radiation sensitivity in prostate cancer.

Oncogene

Madhav A,Andres A,Duong F,Mishra R,Haldar S,Liu Z,Angara B,Gottlieb R,Zumsteg ZS,Bhowmick NA

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2018

Differential stem cell aging kinetics in Hutchinson-Gilford progeria syndrome and Werner syndrome.

Protein & cell

Wu Z,Zhang W,Song M,Wang W,Wei G,Li W,Lei J,Huang Y,Sang Y,Chan P,Chen C,Qu J,Suzuki K,Belmonte JCI,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

BNIP3/Bcl-2-mediated apoptosis induced by cyclic tensile stretch in human cartilage endplate-derived stem cells.

Experimental and therapeutic medicine

Yuan C,Pu L,He Z,Wang J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling.

Nature cell biology

Jin Y,Muhl L,Burmakin M,Wang Y,Duchez AC,Betsholtz C,Arthur HM,Jakobsson L

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Western Blot

Thu Jun 01 00:00:00 EDT 2017

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.

Stem cell research & therapy

Li J,Mao Q,He J,She H,Zhang Z,Yin C

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

The Improvement of Respiratory Performance After Phototherapy-Induced EPC Mobilization in Preterm Infants With RDS.

Journal of cellular biochemistry

Siavashi V,Asadian S,Taheri-Asl M,Babaei H,Keshavarz S,Bazaei M,Nassiri SM

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Circulation Enrichment of Functional Endothelial Progenitor Cells by Infantile Phototherapy.

Journal of cellular biochemistry

Siavashi V,Asadian S,Sharifi A,Esmaeilivand M,Norouzinia R,Azadbakht M,Nassiri SM

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Etanercept-Synthesising Mesenchymal Stem Cells Efficiently Ameliorate Collagen-Induced Arthritis.

Scientific reports

Park N,Rim YA,Jung H,Kim J,Yi H,Kim Y,Jang Y,Jung SM,Lee J,Kwok SK,Park SH,Ju JH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Fri Jan 13 00:00:00 EST 2017

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.

Stem cell reports

Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification.

Stem cells (Dayton, Ohio)

Agarwal S,Loder SJ,Sorkin M,Li S,Shrestha S,Zhao B,Mishina Y,James AW,Levi B

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells.

Scientific reports

Zou L,Chen Q,Quanbeck Z,Bechtold JE,Kaufman DS

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Mar 16 00:00:00 EDT 2016

Influence of aging on the quantity and quality of human cardiac stem cells.

Scientific reports

Nakamura T,Hosoyama T,Kawamura D,Takeuchi Y,Tanaka Y,Samura M,Ueno K,Nishimoto A,Kurazumi H,Suzuki R,Ito H,Sakata K,Mikamo A,Li TS,Hamano K

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Mon Mar 07 00:00:00 EST 2016

SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2.

Cell research

Pan H,Guan D,Liu X,Li J,Wang L,Wu J,Zhou J,Zhang W,Ren R,Zhang W,Li Y,Yang J,Hao Y,Yuan T,Yuan G,Wang H,Ju Z,Mao Z,Li J,Qu J,Tang F,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.

Stem cells international

Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2016

Therapeutic Efficacy of Fresh, Autologous Mesenchymal Stem Cells for Severe Refractory Gingivostomatitis in Cats.

Stem cells translational medicine

Arzi B,Mills-Ko E,Verstraete FJ,Kol A,Walker NJ,Badgley MR,Fazel N,Murphy WJ,Vapniarsky N,Borjesson DL

16-1057 was used in Flow cytometry/Cell sorting to demonstrate the efficacy of adipose-derived stem cell systemic therapy for a naturally occurring, chronic inflammatory disease in cats.

Fri Jan 01 00:00:00 EST 2016

Impaired function of bone marrow stromal cells in systemic mastocytosis.

Stem cell research

Nemeth K,Wilson TM,Ren JJ,Sabatino M,Stroncek DM,Krepuska M,Bai Y,Robey PG,Metcalfe DD,Mezey E

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

Human Gingival Integration-Free iPSCs; a Source for MSC-Like Cells.

International journal of molecular sciences

Umezaki Y,Hashimoto Y,Nishishita N,Kawamata S,Baba S

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model.

Stem cell research & therapy

Zhou F,Gao S,Wang L,Sun C,Chen L,Yuan P,Zhao H,Yi Y,Qin Y,Dong Z,Cao L,Ren H,Zhu L,Li Q,Lu B,Liang A,Xu GT,Zhu H,Gao Z,Ma J,Xu J,Chen X

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Sat May 09 00:00:00 EDT 2015

Modeling familial cancer with induced pluripotent stem cells.

Cell

Lee DF,Su J,Kim HS,Chang B,Papatsenko D,Zhao R,Yuan Y,Gingold J,Xia W,Darr H,Mirzayans R,Hung MC,Schaniel C,Lemischka IR

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu Apr 09 00:00:00 EDT 2015

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu Dec 11 00:00:00 EST 2014

Endothelial microparticles interact with and support the proliferation of T cells.

Journal of immunology (Baltimore, Md. : 1950)

Wheway J,Latham SL,Combes V,Grau GE

16-1057 was used in Flow cytometry/Cell sorting to explore the effect of endothelial cell microparticles in T cell activation and antigen presentation.

Wed Oct 01 00:00:00 EDT 2014

Serum- and growth-factor-free three-dimensional culture system supports cartilage tissue formation by promoting collagen synthesis via Sox9-Col2a1 interaction.

Tissue engineering. Part A

Ahmed N,Iu J,Brown CE,Taylor DW,Kandel RA

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2014

Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.

Tissue engineering. Part A

Mendez JJ,Ghaedi M,Steinbacher D,Niklason LE

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Sun Jun 01 00:00:00 EDT 2014

Endoglin requirement for BMP9 signaling in endothelial cells reveals new mechanism of action for selective anti-endoglin antibodies.

PloS one

Nolan-Stevaux O,Zhong W,Culp S,Shaffer K,Hoover J,Wickramasinghe D,Ruefli-Brasse A

16-1057 was used in a Functional assay to investigate the relative contribution of TGFβ and BMP9 to SMAD1/5/8 activation, and the requirement of endoglin as a co-mediator of SMAD phosphorylation in endothelial cells.

Wed Jul 17 00:00:00 EDT 2013

Early intervention with gene-modified mesenchymal stem cells overexpressing interleukin-4 enhances anti-inflammatory responses and functional recovery in experimental autoimmune demyelination.

Cell adhesion & migration

Payne NL,Dantanarayana A,Sun G,Moussa L,Caine S,McDonald C,Herszfeld D,Bernard CC,Siatskas C

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Dec 19 00:00:00 EST 2012

Pleural mesothelioma side populations have a precursor phenotype.

Carcinogenesis

Frei C,Opitz I,Soltermann A,Fischer B,Moura U,Rehrauer H,Weder W,Stahel R,Felley-Bosco E

16-1057 was used in Flow cytometry/Cell sorting to support the hypothesis that MPM CD105(-)(/low), chemoresistant small sized SP cells may constitute the cellular pool out of which recurrence develops.

Thu Sep 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD105; CD105 antigen; Endoglin; ORW

Gene Aliases: END; ENG; HHT1; ORW1

UniProt ID: (Human) P17813

Entrez Gene ID: (Human) 2022

Molecular Function: transmembrane signal receptor

Function(s)

chronological cell aging patterning of blood vessels vasculogenesis positive regulation of protein phosphorylation heart looping sprouting angiogenesis endocardial cushion morphogenesis cardiac ventricle morphogenesis cardiac atrium morphogenesis ventricular trabecula myocardium morphogenesis cell migration involved in endocardial cushion formation regulation of transcription, DNA-templated cell adhesion negative regulation of gene expression positive regulation of pathway-restricted SMAD protein phosphorylation regulation of transforming growth factor beta receptor signaling pathway central nervous system vasculogenesis negative regulation of cell migration BMP signaling pathway positive regulation of BMP signaling pathway negative regulation of protein autophosphorylation dorsal aorta morphogenesis positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter smooth muscle tissue development artery morphogenesis venous blood vessel morphogenesis endocardial cushion to mesenchymal transition positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation

Process(es)

transmembrane signaling receptor activity transforming growth factor beta receptor, cytoplasmic mediator activity type II transforming growth factor beta receptor binding protein binding galactose binding glycosaminoglycan binding type I transforming growth factor beta receptor binding BMP binding protein homodimerization activity activin binding transforming growth factor beta binding

CD105 (Endoglin) Monoclonal Antibody (SN6), Functional Grade, eBioscience™

Product Details

16-1057-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

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Purification

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Product Specific Information

Target Information

Bioinformatics

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