Invitrogen

CD105 (Endoglin) Monoclonal Antibody (SN6), Functional Grade, eBioscience™

Product Image Gallery

CD105 (Endoglin) Antibody in Immunocytochemistry (ICC/IF) — Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Immunocytochemistry

CD105 (Endoglin) Antibody in Western Blot (WB) — Figure 7 ENG affects VEGFR2 trafficking and downstream signalling. ( a ) Immunoprecipitation of VEGFR2 with protein samples from VEGF-treated HDMECs lenti-virally transduced with either control shRNA or shRNA targeting ENG. Phosphorylated VEGFR2 was detected by blotting with pTyr antibody. ( b ) Ratio of Phosphorylated/total VEGFR2 was measured by ImageJ, n=4 independent experiments. ( c, d ) Western blot of total cell lysates of HDMECs, indicates knockdown of ENG in the sh ENG condition (non-reduced condition). VEGFA treatment for indicated time points reveals differential loss of VEGFR2 over time in control versus knockdown cells as shown by densitometric analysis relative to the loading control calnexin. P <0.001, 2-way ANOVA, n=3 independent experiments. ( e ) Total cell lysates of the above analysed for VEGFR2 downstream components p*AKT, pan AKT, p*ERK1/2 and the loading control calnexin. ( f, g ) Densitometric quantification of p*AKT/total AKT or p*ERK1/2 /total ERK showing potentiated AKT phosphorylation in ENG knockdown ECs ( P <0.001, 2-way ANOVA, n=7 independent experiments for time points 0, 10, 30 min; n=3 independent experiments for time points 5, 15, 20 min) and no difference in p* ERK1/2. ( h ) Cellular membrane proteins were isolated at different time points and analysed for VEGFR2 (note the absence of the intracellular unglycosylated band), ENG, p*Y and total amount (biotin). ( i ) Densitometric analysis of membrane localised VEGFR2 in ( h ). Red or green do

CD105 (Endoglin) Antibody in Flow Cytometry (Flow) — Fig. 1 Characterization of a healthy donor and GS-2 BM-MSCs. a Morphology before and after differentiation of healthy donor (left, n =3) and GS-2 (right, n =3) BM-MSCs. MSCs were cultured in control medium (left lines) or adipogenic and osteogenic differentiation media for 21 days and were stained with Oil Red O (midline, ORO) for adipogenic differentiation and Alizarin Red S (right lines, ARS) for osteogenic differentiation. b Immunophenotype of a healthy donor and GS-2 BM-MSCs. Dot plots from representative BM-MSC samples of passage 3 GS-2 (upper lane) and healthy control (lower lane) BM-MSCs

Product Details

16-1057-82

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	-	View 34 publications 34 publications
Functional Assay (FN)	Assay-Dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Cat, Fruit fly, Human, Mouse
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	SN6
Conjugate	Functional Grade APC APC-eFluor 780 eFluor 450 PE PE-Cyanine7 Super Bright 436 Super Bright 600 Super Bright 645 Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	no preservative
Storage conditions	4° C
RRID	AB_529467

Product Specific Information

Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin. CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.

Applications Reported: This SN6 antibody has been reported to have functional activity.

Applications Tested: This SN6 antibody has been tested by flow cytometric analysis of U937 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Therapeutic Effects of Human Urine-Derived Stem Cells in a Rat Model of Cisplatin-Induced Acute Kidney Injury In Vivo and In Vitro.

Stem cells international

Sun B,Luo X,Yang C,Liu P,Yang Y,Dong X,Yang Z,Xu J,Zhang Y,Li L

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Development, characterization, and hematopoietic differentiation of Griscelli syndrome type 2 induced pluripotent stem cells.

Stem cell research & therapy

Güney-Esken G,Erol ÖD,Pervin B,Gürhan Sevinç G,Önder T,Bilgiç E,Korkusuz P,Günel-Özcan A,Uçkan-Çetinkaya D,Aerts-Kaya F

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

The Effect of Angiotensin II, Retinoic Acid, EGCG, and Vitamin C on the Cardiomyogenic Differentiation Induction of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.

International journal of molecular sciences

Gasiūnienė M,Valatkaitė E,Navakauskaitė A,Navakauskienė R

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu Nov 19 00:00:00 EST 2020

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

ATF6 safeguards organelle homeostasis and cellular aging in human mesenchymal stem cells.

Cell discovery

Wang S,Hu B,Ding Z,Dang Y,Wu J,Li D,Liu X,Xiao B,Zhang W,Ren R,Lei J,Hu H,Chen C,Chan P,Li D,Qu J,Tang F,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

Chondrogenic Differentiation from Induced Pluripotent Stem Cells Using Non-Viral Minicircle Vectors.

Cells

Rim YA,Nam Y,Park N,Jung H,Lee K,Lee J,Ju JH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.

Molecular medicine reports

Fan C,Ji Q,Zhang C,Xu S,Sun H,Li Z

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Telomere-dependent and telomere-independent roles of RAP1 in regulating human stem cell homeostasis.

Protein & cell

Zhang X,Liu Z,Liu X,Wang S,Zhang Y,He X,Sun S,Ma S,Shyh-Chang N,Liu F,Wang Q,Wang X,Liu L,Zhang W,Song M,Liu GH,Qu J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Stabilizing heterochromatin by DGCR8 alleviates senescence and osteoarthritis.

Nature communications

Deng L,Ren R,Liu Z,Song M,Li J,Wu Z,Ren X,Fu L,Li W,Zhang W,Guillen P,Izpisua Belmonte JC,Chan P,Qu J,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Fri Jul 26 00:00:00 EDT 2019

Chemical screen identifies a geroprotective role of quercetin in premature aging.

Protein & cell

Geng L,Liu Z,Zhang W,Li W,Wu Z,Wang W,Ren R,Su Y,Wang P,Sun L,Ju Z,Chan P,Song M,Qu J,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Utilizing Confocal Microscopy to Characterize Human and Mouse Adipose Tissue.

Tissue engineering. Part C, Methods

Blackshear CP,Borrelli MR,Shen EZ,Ransom RC,Chung NN,Vistnes SM,Irizarry D,Nazerali R,Momeni A,Longaker MT,Wan DC

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Immunocytochemistry

Mon Oct 01 00:00:00 EDT 2018

BNIP3/Bcl-2-mediated apoptosis induced by cyclic tensile stretch in human cartilage endplate-derived stem cells.

Experimental and therapeutic medicine

Yuan C,Pu L,He Z,Wang J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling.

Nature cell biology

Jin Y,Muhl L,Burmakin M,Wang Y,Duchez AC,Betsholtz C,Arthur HM,Jakobsson L

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Western Blot

Thu Jun 01 00:00:00 EDT 2017

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.

Stem cell research & therapy

Li J,Mao Q,He J,She H,Zhang Z,Yin C

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

Etanercept-Synthesising Mesenchymal Stem Cells Efficiently Ameliorate Collagen-Induced Arthritis.

Scientific reports

Park N,Rim YA,Jung H,Kim J,Yi H,Kim Y,Jang Y,Jung SM,Lee J,Kwok SK,Park SH,Ju JH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Fri Jan 13 00:00:00 EST 2017

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.

Stem cell reports

Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification.

Stem cells (Dayton, Ohio)

Agarwal S,Loder SJ,Sorkin M,Li S,Shrestha S,Zhao B,Mishina Y,James AW,Levi B

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells.

Scientific reports

Zou L,Chen Q,Quanbeck Z,Bechtold JE,Kaufman DS

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Mar 16 00:00:00 EDT 2016

Influence of aging on the quantity and quality of human cardiac stem cells.

Scientific reports

Nakamura T,Hosoyama T,Kawamura D,Takeuchi Y,Tanaka Y,Samura M,Ueno K,Nishimoto A,Kurazumi H,Suzuki R,Ito H,Sakata K,Mikamo A,Li TS,Hamano K

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Mon Mar 07 00:00:00 EST 2016

SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2.

Cell research

Pan H,Guan D,Liu X,Li J,Wang L,Wu J,Zhou J,Zhang W,Ren R,Zhang W,Li Y,Yang J,Hao Y,Yuan T,Yuan G,Wang H,Ju Z,Mao Z,Li J,Qu J,Tang F,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.

Stem cells international

Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2016

Therapeutic Efficacy of Fresh, Autologous Mesenchymal Stem Cells for Severe Refractory Gingivostomatitis in Cats.

Stem cells translational medicine

Arzi B,Mills-Ko E,Verstraete FJ,Kol A,Walker NJ,Badgley MR,Fazel N,Murphy WJ,Vapniarsky N,Borjesson DL

16-1057 was used in Flow cytometry/Cell sorting to demonstrate the efficacy of adipose-derived stem cell systemic therapy for a naturally occurring, chronic inflammatory disease in cats.

Fri Jan 01 00:00:00 EST 2016

Impaired function of bone marrow stromal cells in systemic mastocytosis.

Stem cell research

Nemeth K,Wilson TM,Ren JJ,Sabatino M,Stroncek DM,Krepuska M,Bai Y,Robey PG,Metcalfe DD,Mezey E

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

Human Gingival Integration-Free iPSCs; a Source for MSC-Like Cells.

International journal of molecular sciences

Umezaki Y,Hashimoto Y,Nishishita N,Kawamata S,Baba S

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model.

Stem cell research & therapy

Zhou F,Gao S,Wang L,Sun C,Chen L,Yuan P,Zhao H,Yi Y,Qin Y,Dong Z,Cao L,Ren H,Zhu L,Li Q,Lu B,Liang A,Xu GT,Zhu H,Gao Z,Ma J,Xu J,Chen X

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Sat May 09 00:00:00 EDT 2015

Modeling familial cancer with induced pluripotent stem cells.

Cell

Lee DF,Su J,Kim HS,Chang B,Papatsenko D,Zhao R,Yuan Y,Gingold J,Xia W,Darr H,Mirzayans R,Hung MC,Schaniel C,Lemischka IR

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu Apr 09 00:00:00 EDT 2015

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Thu Dec 11 00:00:00 EST 2014

Endothelial microparticles interact with and support the proliferation of T cells.

Journal of immunology (Baltimore, Md. : 1950)

Wheway J,Latham SL,Combes V,Grau GE

16-1057 was used in Flow cytometry/Cell sorting to explore the effect of endothelial cell microparticles in T cell activation and antigen presentation.

Wed Oct 01 00:00:00 EDT 2014

Serum- and growth-factor-free three-dimensional culture system supports cartilage tissue formation by promoting collagen synthesis via Sox9-Col2a1 interaction.

Tissue engineering. Part A

Ahmed N,Iu J,Brown CE,Taylor DW,Kandel RA

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2014

Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.

Tissue engineering. Part A

Mendez JJ,Ghaedi M,Steinbacher D,Niklason LE

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Sun Jun 01 00:00:00 EDT 2014

Endoglin requirement for BMP9 signaling in endothelial cells reveals new mechanism of action for selective anti-endoglin antibodies.

PloS one

Nolan-Stevaux O,Zhong W,Culp S,Shaffer K,Hoover J,Wickramasinghe D,Ruefli-Brasse A

16-1057 was used in a Functional assay to investigate the relative contribution of TGFβ and BMP9 to SMAD1/5/8 activation, and the requirement of endoglin as a co-mediator of SMAD phosphorylation in endothelial cells.

Wed Jul 17 00:00:00 EDT 2013

Early intervention with gene-modified mesenchymal stem cells overexpressing interleukin-4 enhances anti-inflammatory responses and functional recovery in experimental autoimmune demyelination.

Cell adhesion & migration

Payne NL,Dantanarayana A,Sun G,Moussa L,Caine S,McDonald C,Herszfeld D,Bernard CC,Siatskas C

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 16-1057-82) in Flow Cytometry

Wed Dec 19 00:00:00 EST 2012

Pleural mesothelioma side populations have a precursor phenotype.

Carcinogenesis

Frei C,Opitz I,Soltermann A,Fischer B,Moura U,Rehrauer H,Weder W,Stahel R,Felley-Bosco E

16-1057 was used in Flow cytometry/Cell sorting to support the hypothesis that MPM CD105(-)(/low), chemoresistant small sized SP cells may constitute the cellular pool out of which recurrence develops.