Invitrogen

CD105 (Endoglin) Monoclonal Antibody (SN6), PE-Cyanine7, eBioscience™

Product Image Gallery

CD105 (Endoglin) Antibody in Flow Cytometry (Flow) — Staining of U937 cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD105 (Endoglin) PE-Cyanine7 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.

Product Details

25-1057-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.125 µg)/test	View 33 publications 33 publications

Product Specifications
Species Reactivity	Human
Published species	Dog, Fruit fly, Human
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	SN6
Conjugate	PE-Cyanine7 APC APC-eFluor 780 eFluor 450 Functional Grade PE Super Bright 436 Super Bright 600 Super Bright 645 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_11217682

Product Specific Information

Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin. CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.

Applications Reported: This SN6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SN6 antibody has been pre-titrated and tested by flow cytometric analysis of the U937 cell line. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Therapeutic Effects of Human Urine-Derived Stem Cells in a Rat Model of Cisplatin-Induced Acute Kidney Injury In Vivo and In Vitro.

Stem cells international

Sun B,Luo X,Yang C,Liu P,Yang Y,Dong X,Yang Z,Xu J,Zhang Y,Li L

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Development, characterization, and hematopoietic differentiation of Griscelli syndrome type 2 induced pluripotent stem cells.

Stem cell research & therapy

Güney-Esken G,Erol ÖD,Pervin B,Gürhan Sevinç G,Önder T,Bilgiç E,Korkusuz P,Günel-Özcan A,Uçkan-Çetinkaya D,Aerts-Kaya F

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

The Effect of Angiotensin II, Retinoic Acid, EGCG, and Vitamin C on the Cardiomyogenic Differentiation Induction of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.

International journal of molecular sciences

Gasiūnienė M,Valatkaitė E,Navakauskaitė A,Navakauskienė R

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Thu Nov 19 00:00:00 EST 2020

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

ATF6 safeguards organelle homeostasis and cellular aging in human mesenchymal stem cells.

Cell discovery

Wang S,Hu B,Ding Z,Dang Y,Wu J,Li D,Liu X,Xiao B,Zhang W,Ren R,Lei J,Hu H,Chen C,Chan P,Li D,Qu J,Tang F,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

Chondrogenic Differentiation from Induced Pluripotent Stem Cells Using Non-Viral Minicircle Vectors.

Cells

Rim YA,Nam Y,Park N,Jung H,Lee K,Lee J,Ju JH

25-1057 was used in Flow cytometry/Cell sorting to explore the use of minicircle vectors in cartilage regeneration using human induced pluripotent stem cells.

Sun Mar 01 00:00:00 EST 2020

Chondrogenic Differentiation from Induced Pluripotent Stem Cells Using Non-Viral Minicircle Vectors.

Cells

Rim YA,Nam Y,Park N,Jung H,Lee K,Lee J,Ju JH

25-1057 was used in Flow cytometry/Cell sorting to explore the use of minicircle vectors in cartilage regeneration using human induced pluripotent stem cells.

Sun Mar 01 00:00:00 EST 2020

Immunosuppressive Mesenchymal Stromal Cells Derived from Human-Induced Pluripotent Stem Cells Induce Human Regulatory T Cells In Vitro and In Vivo.

Frontiers in immunology

Roux C,Saviane G,Pini J,Belaïd N,Dhib G,Voha C,Ibáñez L,Boutin A,Mazure NM,Wakkach A,Blin-Wakkach C,Rouleau M

25-1057 was used in Flow cytometry/Cell sorting to examine the immunosuppressive activity of human induced pluripotent stem cell derived-mesenchymal stromal cells on human T-cell stimulation with a concomitant generation of human regulatory T cells in vivo.

Wed Nov 20 00:00:00 EST 2019

TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.

Molecular medicine reports

Fan C,Ji Q,Zhang C,Xu S,Sun H,Li Z

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Telomere-dependent and telomere-independent roles of RAP1 in regulating human stem cell homeostasis.

Protein & cell

Zhang X,Liu Z,Liu X,Wang S,Zhang Y,He X,Sun S,Ma S,Shyh-Chang N,Liu F,Wang Q,Wang X,Liu L,Zhang W,Song M,Liu GH,Qu J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Stabilizing heterochromatin by DGCR8 alleviates senescence and osteoarthritis.

Nature communications

Deng L,Ren R,Liu Z,Song M,Li J,Wu Z,Ren X,Fu L,Li W,Zhang W,Guillen P,Izpisua Belmonte JC,Chan P,Qu J,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Fri Jul 26 00:00:00 EDT 2019

Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.

Experimental and therapeutic medicine

Yan N,Xu J,Zhao C,Wu Y,Gao F,Li C,Zhou W,Xiao T,Zhou X,Shao Q,Xia S

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Adenovirus-Mediated Expression of BMP-2 and BFGF in Bone Marrow Mesenchymal Stem Cells Combined with Demineralized Bone Matrix For Repair of Femoral Head Osteonecrosis in Beagle Dogs.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Peng WX,Wang L

25-1057 was used in Flow cytometry/Cell sorting to indicate that the use of Ad-BMP-2/bFGF-modified BMSCs in conjunction with DBM could successfully repair ONFH in a dog model by promoting bone formation and angiogenesis.

Mon Dec 18 00:00:00 EST 2017

Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces β-cell proliferation in diabetic mice.

International journal of molecular medicine

Sun LL,Liu TJ,Li L,Tang W,Zou JJ,Chen XF,Zheng JY,Jiang BG,Shi YQ

25-1057 was used in Flow cytometry/Cell sorting to demonstrate a novel, betatrophin-based strategy for inducing β-cell regeneration.

Sat Apr 01 00:00:00 EDT 2017

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.

Stem cell research & therapy

Li J,Mao Q,He J,She H,Zhang Z,Yin C

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

Etanercept-Synthesising Mesenchymal Stem Cells Efficiently Ameliorate Collagen-Induced Arthritis.

Scientific reports

Park N,Rim YA,Jung H,Kim J,Yi H,Kim Y,Jang Y,Jung SM,Lee J,Kwok SK,Park SH,Ju JH

25-1057 was used in Flow cytometry/Cell sorting to develop a novel method to produce synthetic biological drugs from engineered MSCs, and investigate the anti-arthritic effect of these cells in a collagen-induced arthritis mouse model.

Fri Jan 13 00:00:00 EST 2017

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Spontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold.

PloS one

Sonomoto K,Yamaoka K,Kaneko H,Yamagata K,Sakata K,Zhang X,Kondo M,Zenke Y,Sabanai K,Nakayamada S,Sakai A,Tanaka Y

25-1057 was used in Flow cytometry/Cell sorting to investigate the combination of MSCs and NFs as a therapeutic technique for the repair of joints affected by bone and cartilage destruction.

Thu Sep 01 00:00:00 EDT 2016

MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.

Stem cell reports

Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells.

Scientific reports

Zou L,Chen Q,Quanbeck Z,Bechtold JE,Kaufman DS

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Wed Mar 16 00:00:00 EDT 2016

Influence of aging on the quantity and quality of human cardiac stem cells.

Scientific reports

Nakamura T,Hosoyama T,Kawamura D,Takeuchi Y,Tanaka Y,Samura M,Ueno K,Nishimoto A,Kurazumi H,Suzuki R,Ito H,Sakata K,Mikamo A,Li TS,Hamano K

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Mon Mar 07 00:00:00 EST 2016

SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2.

Cell research

Pan H,Guan D,Liu X,Li J,Wang L,Wu J,Zhou J,Zhang W,Ren R,Zhang W,Li Y,Yang J,Hao Y,Yuan T,Yuan G,Wang H,Ju Z,Mao Z,Li J,Qu J,Tang F,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.

Stem cells international

Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2016

Impaired function of bone marrow stromal cells in systemic mastocytosis.

Stem cell research

Nemeth K,Wilson TM,Ren JJ,Sabatino M,Stroncek DM,Krepuska M,Bai Y,Robey PG,Metcalfe DD,Mezey E

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

Human Gingival Integration-Free iPSCs; a Source for MSC-Like Cells.

International journal of molecular sciences

Umezaki Y,Hashimoto Y,Nishishita N,Kawamata S,Baba S

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model.

Stem cell research & therapy

Zhou F,Gao S,Wang L,Sun C,Chen L,Yuan P,Zhao H,Yi Y,Qin Y,Dong Z,Cao L,Ren H,Zhu L,Li Q,Lu B,Liang A,Xu GT,Zhu H,Gao Z,Ma J,Xu J,Chen X

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Sat May 09 00:00:00 EDT 2015

Modeling familial cancer with induced pluripotent stem cells.

Cell

Lee DF,Su J,Kim HS,Chang B,Papatsenko D,Zhao R,Yuan Y,Gingold J,Xia W,Darr H,Mirzayans R,Hung MC,Schaniel C,Lemischka IR

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1057-42) in Flow Cytometry

Thu Apr 09 00:00:00 EDT 2015

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A