Invitrogen

CD105 (Endoglin) Monoclonal Antibody (SN6), Super Bright™ 645, eBioscience™

Product Image Gallery

CD105 (Endoglin) Antibody in Flow Cytometry (Flow) — Staining of U937 cells with Mouse IgG1 K Isotype Control Super Bright 645 (Product # 64-4714-82) (blue histogram) or CD105 (Endoglin) Monoclonal Antibody, Super Bright 645 (purple histogram). Total viable cells were used for analysis.

CD105 (Endoglin) Antibody in Immunocytochemistry (ICC/IF) — Immunostaining of human FF cells for specific cell surface markers after 48h. (a) Red arrows show cells low positive expression of CD105, (c) negative expression of CD34. Cell nuclei were counterstained by DAPI (b, d); 20X.

Product Details

64-1057-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.06 µg)/test	View 23 publications 23 publications
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 645, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	SN6
Conjugate	Super Bright™ 645 APC APC-eFluor 780 eFluor 450 Functional Grade PE PE-Cyanine7 Super Bright 436 Super Bright 600 Unconjugated
Excitation/Emission Max	414/645 nm View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2717099

Product Specific Information

Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin. CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.

Applications Reported: The SN6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SN6 antibody has been pre-titrated and tested by flow cytometric analysis of U937 cell line. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Human Amniotic Mesenchymal Stem Cells Inhibit aGVHD by Regulating Balance of Treg and T Effector Cells.

Journal of inflammation research

Gao Y,Li W,Bu X,Xu Y,Cai S,Zhong J,Du M,Sun H,Huang L,He Y,Hu X,Liu Q,Jin H,Wang Q,Ping B

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Tue Apr 26 00:00:00 EDT 2022

The Effect of Different Doses of Melatonin on in Vitro Maturation of Human Follicular Fluid-Derived Oocyte-Like Cells.

JBRA assisted reproduction

Azandeh S,Moghadam MT,Rashno M,Zargar M,Zadeh PA

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Immunocytochemistry

Sun Apr 17 00:00:00 EDT 2022

Exosomes derived from stem cells of human deciduous exfoliated teeth inhibit angiogenesis in vivo and in vitro via the transfer of miR-100-5p and miR-1246.

Stem cell research & therapy

Liu P,Zhang Q,Mi J,Wang S,Xu Q,Zhuang D,Chen W,Liu C,Zhang L,Guo J,Wu X

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Thu Mar 03 00:00:00 EST 2022

Induced Neurodifferentiation of hBM-MSCs through Activation of the ERK/CREB Pathway via Pulsed Electromagnetic Fields and Physical Stimulation Promotes Neurogenesis in Cerebral Ischemic Models.

International journal of molecular sciences

Park HJ,Choi JH,Nam MH,Seo YK

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Fri Jan 21 00:00:00 EST 2022

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Scientific reports

Chen C,Huang X,Zhu W,Ding C,Huang P,Li R

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model.

Stem cell research & therapy

Li Y,Shi G,Han Y,Shang H,Li H,Liang W,Zhao W,Bai L,Qin C

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Thu Jul 15 00:00:00 EDT 2021

Development, characterization, and hematopoietic differentiation of Griscelli syndrome type 2 induced pluripotent stem cells.

Stem cell research & therapy

Güney-Esken G,Erol ÖD,Pervin B,Gürhan Sevinç G,Önder T,Bilgiç E,Korkusuz P,Günel-Özcan A,Uçkan-Çetinkaya D,Aerts-Kaya F

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Fri Nov 27 00:00:00 EST 2020

The Effect of Angiotensin II, Retinoic Acid, EGCG, and Vitamin C on the Cardiomyogenic Differentiation Induction of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.

International journal of molecular sciences

Gasiūnienė M,Valatkaitė E,Navakauskaitė A,Navakauskienė R

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Thu Nov 19 00:00:00 EST 2020

Vasculogenesis from Human Dental Pulp Stem Cells Grown in Matrigel with Fully Defined Serum-Free Culture Media.

Biomedicines

Luzuriaga J,Irurzun J,Irastorza I,Unda F,Ibarretxe G,Pineda JR

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Mon Nov 09 00:00:00 EST 2020

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

ZKSCAN3 counteracts cellular senescence by stabilizing heterochromatin.

Nucleic acids research

Hu H,Ji Q,Song M,Ren J,Liu Z,Wang Z,Liu X,Yan K,Hu J,Jing Y,Wang S,Zhang W,Liu GH,Qu J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2020

Resveratrol rescues TNF‑α‑induced inhibition of osteogenesis in human periodontal ligament stem cells via the ERK1/2 pathway.

Molecular medicine reports

Yuan J,Wang X,Ma D,Gao H,Zheng D,Zhang J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

Chondrogenic Differentiation from Induced Pluripotent Stem Cells Using Non-Viral Minicircle Vectors.

Cells

Rim YA,Nam Y,Park N,Jung H,Lee K,Lee J,Ju JH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction.

Protein & cell

Wang S,Min Z,Ji Q,Geng L,Su Y,Liu Z,Hu H,Wang L,Zhang W,Suzuiki K,Huang Y,Zhang P,Tang TS,Qu J,Yu Y,Liu GH,Qiao J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer.

Cancers

Huang JY,Wang YY,Lo S,Tseng LM,Chen DR,Wu YC,Hou MF,Yuan SF

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Fri Dec 20 00:00:00 EST 2019

Up-regulation of FOXD1 by YAP alleviates senescence and osteoarthritis.

PLoS biology

Fu L,Hu Y,Song M,Liu Z,Zhang W,Yu FX,Wu J,Wang S,Izpisua Belmonte JC,Chan P,Qu J,Tang F,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

Utilizing Confocal Microscopy to Characterize Human and Mouse Adipose Tissue.

Tissue engineering. Part C, Methods

Blackshear CP,Borrelli MR,Shen EZ,Ransom RC,Chung NN,Vistnes SM,Irizarry D,Nazerali R,Momeni A,Longaker MT,Wan DC

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Immunocytochemistry

Mon Oct 01 00:00:00 EDT 2018

Antagonizing CD105 enhances radiation sensitivity in prostate cancer.

Oncogene

Madhav A,Andres A,Duong F,Mishra R,Haldar S,Liu Z,Angara B,Gottlieb R,Zumsteg ZS,Bhowmick NA

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2018

Differential stem cell aging kinetics in Hutchinson-Gilford progeria syndrome and Werner syndrome.

Protein & cell

Wu Z,Zhang W,Song M,Wang W,Wei G,Li W,Lei J,Huang Y,Sang Y,Chan P,Chen C,Qu J,Suzuki K,Belmonte JCI,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

The Improvement of Respiratory Performance After Phototherapy-Induced EPC Mobilization in Preterm Infants With RDS.

Journal of cellular biochemistry

Siavashi V,Asadian S,Taheri-Asl M,Babaei H,Keshavarz S,Bazaei M,Nassiri SM

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Circulation Enrichment of Functional Endothelial Progenitor Cells by Infantile Phototherapy.

Journal of cellular biochemistry

Siavashi V,Asadian S,Sharifi A,Esmaeilivand M,Norouzinia R,Azadbakht M,Nassiri SM

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Bioinformatics

Protein Aliases: CD105; CD105 antigen; Endoglin; ORW

Gene Aliases: END; ENG; HHT1; ORW1

UniProt ID: (Human) P17813

Entrez Gene ID: (Human) 2022

Molecular Function: transmembrane signal receptor

Function(s)

chronological cell aging patterning of blood vessels vasculogenesis positive regulation of protein phosphorylation heart looping sprouting angiogenesis endocardial cushion morphogenesis cardiac ventricle morphogenesis cardiac atrium morphogenesis ventricular trabecula myocardium morphogenesis cell migration involved in endocardial cushion formation regulation of transcription, DNA-templated cell adhesion negative regulation of gene expression positive regulation of pathway-restricted SMAD protein phosphorylation regulation of transforming growth factor beta receptor signaling pathway central nervous system vasculogenesis negative regulation of cell migration BMP signaling pathway positive regulation of BMP signaling pathway negative regulation of protein autophosphorylation dorsal aorta morphogenesis positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter smooth muscle tissue development artery morphogenesis venous blood vessel morphogenesis endocardial cushion to mesenchymal transition positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation

Process(es)

transmembrane signaling receptor activity transforming growth factor beta receptor, cytoplasmic mediator activity type II transforming growth factor beta receptor binding protein binding galactose binding glycosaminoglycan binding type I transforming growth factor beta receptor binding BMP binding protein homodimerization activity activin binding transforming growth factor beta binding

CD105 (Endoglin) Monoclonal Antibody (SN6), Super Bright™ 645, eBioscience™

Product Details

64-1057-42

Flow Cytometry (Flow)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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