Invitrogen

CD106 (VCAM-1) Monoclonal Antibody (429), Biotin, eBioscience™

CD106 (VCAM-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

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CD106 (VCAM-1) Antibody (13-1061-82) in ICC/IF, Flow

Nature communications 2017 - Figure 3 Loss of endothelial SENP1 inhibits EC activation. ( a ) Grafts from WT or SENP1-ecKO mice were harvested 3 days post-transplantation. The induction of endothelial adhesion molecules was demonstrated by immunofluorescence staining of ICAM-1, VCAM-1, or P-selectin and PECAM-1 with DAPI labelling of the nuclei. Bar represents 50 mum. ( b - e ) Attenuated induction of adhesion molecules in SENP1-ecKO M... View More

Product Image Gallery

CD106 (VCAM-1) Antibody in Immunohistochemistry (IHC) — Fig. 4 GRP78 in hepatocytes. Immunocyte-fluorescent study of GRP78 was done for primary murine hepatocytes after incubation for 24 h in either media alone (CNT group), media containing 0.2 mM palmitate (FFA group), or media containing 0.2 mM palmitate and 3 muM dantrolene (FFA + DAN group). A: Representative images of immunocyte-fluorescent study for GRP78 hepatocytes. B: GRP78 fluorescent intensity were calculated. There were significant differences between FFA group and FFA + DAN group. Values are the mean +- SEM of 60 cells from three different experiments. Fig. 4

CD106 (VCAM-1) Antibody in Flow Cytometry (Flow) — Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Flow Cytometry

Product Details

13-1061-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.25 µg/test

View 13 publications 13 publications

Functional Assay (FN)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

429

Conjugate

Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_466559

Product Specific Information

Description: The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin a4b7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported: The 429 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 429 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An Agonistic Anti-CD137 Antibody Disrupts Lymphoid Follicle Structure and T-Cell-Dependent Antibody Responses.

Cell reports. Medicine

Hong JP,Reynoso GV,Andhey PS,Swain A,Turner JS,Boon ACM,Krammer F,Ellebedy AH,Zanini F,Artyomov M,Hickman HD,Diamond MS

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Immunohistochemistry

Tue Jun 23 00:00:00 EDT 2020

Dantrolene prevents hepatic steatosis by reducing cytoplasmic Ca²⁺ level and ER stress.

Biochemistry and biophysics reports

Tamitani M,Yamamoto T,Yamamoto N,Fujisawa K,Tanaka S,Nakamura Y,Uchinoumi H,Oda T,Okuda S,Takami T,Kobayashi S,Sakaida I,Yano M

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Immunohistochemistry

Tue Sep 01 00:00:00 EDT 2020

Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow.

Nature communications

Riedel R,Addo R,Ferreira-Gomes M,Heinz GA,Heinrich F,Kummer J,Greiff V,Schulz D,Klaeden C,Cornelis R,Menzel U,Kröger S,Stervbo U,Köhler R,Haftmann C,Kühnel S,Lehmann K,Maschmeyer P,McGrath M,Naundorf S,Hahne S,Sercan-Alp Ö,Siracusa F,Stefanowski J,Weber M,Westendorf K,Zimmermann J,Hauser AE,Reddy ST,Durek P,Chang HD,Mashreghi MF,Radbruch A

13-1061 was used in Immunohistochemistry-immunofluorescence to identify distinct populations of isotype-switched memory B lymphocytes of murine spleen and bone marrow.

Fri May 22 00:00:00 EDT 2020

Leptin Induces Proadipogenic and Proinflammatory Signaling in Adipocytes.

Frontiers in endocrinology

Palhinha L,Liechocki S,Hottz ED,Pereira JADS,de Almeida CJ,Moraes-Vieira PMM,Bozza PT,Maya-Monteiro CM

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Flow Cytometry

Mon Jan 13 00:00:00 EST 2020

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche.

Cell reports

Balzano M,De Grandis M,Vu Manh TP,Chasson L,Bardin F,Farina A,Sergé A,Bidaut G,Charbord P,Hérault L,Bailly AL,Cartier-Michaud A,Boned A,Dalod M,Duprez E,Genever P,Coles M,Bajenoff M,Xerri L,Aurrand-Lions M,Schiff C,Mancini SJC

13-1061 was used in Flow cytometry/Cell sorting to study pro-B cells in their support of stroll cells and their interaction network allowing their retention.

Tue Mar 19 00:00:00 EDT 2019

Inhibition of the JNK/MAPK signaling pathway by myogenesis-associated miRNAs is required for skeletal muscle development.

Cell death and differentiation

Xie SJ,Li JH,Chen HF,Tan YY,Liu SR,Zhang Y,Xu H,Yang JH,Liu S,Zheng LL,Huang MB,Guo YH,Zhang Q,Zhou H,Qu LH

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Low-Dose Radiotherapy Has No Harmful Effects on Key Cells of Healthy Non-Inflamed Joints.

International journal of molecular sciences

Deloch L,Rückert M,Fietkau R,Frey B,Gaipl US

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Flow Cytometry

Tue Oct 16 00:00:00 EDT 2018

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Flt3 Ligand Regulates the Development of Innate Lymphoid Cells in Fetal and Adult Mice.

Journal of immunology (Baltimore, Md. : 1950)

Baerenwaldt A,von Burg N,Kreuzaler M,Sitte S,Horvath E,Peter A,Voehringer D,Rolink AG,Finke D

13-1061 was used in Immunohistochemistry to investigate the role of the Flt3 ligand in the development and regulation of innate lymphoid cells in fetal and adult mice.

Tue Mar 15 00:00:00 EDT 2016

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Flow Cytometry

Tue Jun 21 00:00:00 EDT 2016

Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.

Cell

Haldar M,Kohyama M,So AY,Kc W,Wu X,Briseño CG,Satpathy AT,Kretzer NM,Arase H,Rajasekaran NS,Wang L,Egawa T,Igarashi K,Baltimore D,Murphy TL,Murphy KM

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Flow Cytometry

Thu Mar 13 00:00:00 EDT 2014

Effects of intra-abdominal sepsis on atherosclerosis in mice.

Critical care (London, England)

Kaynar AM,Yende S,Zhu L,Frederick DR,Chambers R,Burton CL,Carter M,Stolz DB,Agostini B,Gregory AD,Nagarajan S,Shapiro SD,Angus DC

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Immunofluorescence

Wed Sep 03 00:00:00 EDT 2014

Dominant lethal pathologies in male mice engineered to contain an X-linked DUX4 transgene.

Cell reports

Dandapat A,Bosnakovski D,Hartweck LM,Arpke RW,Baltgalvis KA,Vang D,Baik J,Darabi R,Perlingeiro RC,Hamra FK,Gupta K,Lowe DA,Kyba M

13-1061 was used in Flow cytometry/Cell sorting to demonstrate a murine model with potential use for testing anti-DUX4 therapies in facioscapulohumeral muscular dystrophy.

Thu Sep 11 00:00:00 EDT 2014

Osteocytes regulate primary lymphoid organs and fat metabolism.

Cell metabolism

Sato M,Asada N,Kawano Y,Wakahashi K,Minagawa K,Kawano H,Sada A,Ikeda K,Matsui T,Katayama Y

13-1061 was used in Flow cytometry/Cell sorting to study the effects of osteocyte ablation on the homeostasis of remote organs in mice.

Tue Nov 05 00:00:00 EST 2013

Rhodamine-loaded intercellular adhesion molecule-1-targeted microbubbles for dual-modality imaging under controlled shear stresses.

Circulation. Cardiovascular imaging

Wu Z,Curaj A,Fokong S,Liehn EA,Weber C,Lammers T,Kiessling F,Zandvoort van M

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Immunohistochemistry

Fri Nov 01 00:00:00 EDT 2013

Defining the transcriptional and cellular landscape of type 1 diabetes in the NOD mouse.

PloS one

Carrero JA,Calderon B,Towfic F,Artyomov MN,Unanue ER

13-1061 was used in Immunofluorescence to examine autoimmune diabetes and its disease progression markers.

Mon Sep 30 00:00:00 EDT 2013

The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis.

Nature medicine

Schmidt EP,Yang Y,Janssen WJ,Gandjeva A,Perez MJ,Barthel L,Zemans RL,Bowman JC,Koyanagi DE,Yunt ZX,Smith LP,Cheng SS,Overdier KH,Thompson KR,Geraci MW,Douglas IS,Pearse DB,Tuder RM

13-1061 was used in Functional assays to elucidate the mechanisms by which glycocalyx loss occurs during sepsis, and how this allows for neutrophil adhesion within the pulmonary circulation.

Wed Aug 01 00:00:00 EDT 2012

Requirement for lymphoid tissue-inducer cells in isolated follicle formation and T cell-independent immunoglobulin A generation in the gut.

Immunity

Tsuji M,Suzuki K,Kitamura H,Maruya M,Kinoshita K,Ivanov II,Itoh K,Littman DR,Fagarasan S

13-1061 was used in Immunohistochemistry on frozen tissues to investigate the sites and mechanisms for T cell-independent IgA synthesis.

Fri Aug 15 00:00:00 EDT 2008

Lymphotoxin a-dependent and -independent signals regulate stromal organizer cell homeostasis during lymph node organogenesis.

Blood

White A,Carragher D,Parnell S,Msaki A,Perkins N,Lane P,Jenkinson E,Anderson G,Caamaño JH

13-1061 was used in Flow cytometry/Cell sorting to study LTa-independent and LTa-dependent staages of lymph node development which regulate stromal organizer cell homeostasis.

Sat Sep 15 00:00:00 EDT 2007

T/B lineage choice occurs prior to intrathymic Notch signaling.

Blood

Harman BC,Jenkinson WE,Parnell SM,Rossi SW,Jenkinson EJ,Anderson G

13-1061 was used in Flow cytometry/Cell sorting to analyse the T-cell/B-cell lineage potential of precursors that have been recruited to the foetal mouse thymus, showing that lineage choice occurs prior to intrathymic Notch signaling.

Mon Aug 01 00:00:00 EDT 2005

Differential expression of novel potential regulators in hematopoietic stem cells.

PLoS genetics

Forsberg EC,Prohaska SS,Katzman S,Heffner GC,Stuart JM,Weissman IL

13-1061 was used in Flow cytometry/Cell sorting to compare the expression pattern of three hematopoietic subpopulations in adult mouse bone marrow.

Thu Sep 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: DKFZp779G2333; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; vascular cell adhesion protein 1

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Gene Aliases: CD106; Vcam-1

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Entrez Gene ID: (Mouse) 22329

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Function(s)

cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion heart development amine metabolic process viral process single organismal cell-cell adhesion positive regulation of T cell proliferation leukocyte tethering or rolling oxidation-reduction process cell chemotaxis embryonic placenta morphogenesis chorio-allantoic fusion visceral serous pericardium development cellular response to glucose stimulus

Process(es)

integrin binding primary amine oxidase activity cell adhesion molecule binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD106 (VCAM-1) Monoclonal Antibody (429), Biotin, eBioscience™

CD106 (VCAM-1) Antibody (13-1061-82) in ICC/IF, Flow

Product Details

13-1061-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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