Invitrogen

CD106 (VCAM-1) Monoclonal Antibody (429), Biotin, eBioscience™

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CD106 (VCAM-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Figure 3 Loss of endothelial SENP1 inhibits EC activation. ( a ) Grafts from WT or SENP1-ecKO mice were harvested 3 days post-transplantation. The induction of endothelial adhesion molecules was demonstrated by immunofluorescence staining of ICAM-1, VCAM-1, or P-selectin and PECAM-1 with DAPI labelling of the nuclei. Bar represents 50 mum. ( b - e ) Attenuated induction of adhesion molecules in SENP1-ecKO MAECs. Flow cytometry analysis of ICAM-1, VCAM-1 and P-selectin in MAECs isolated from WT or SENP1-ecKO mice after TNF or IL-1beta treatment. Representative histograms are shown in ( b ) with the quantification of mean intensity in ( c - e ). ( f - h ) Overexpression of the catalytically inactive form of SENP1 (SENP1-Mut) inhibits the induction of adhesion molecules in HUVECs. HUVECs were infected by Ad-SENP1-Mut or vector control (Ad-LacZ) for 24 h, treated with pro-inflammatory cytokines and analysed by flow cytometry in the same way as MAECs. Representative histograms of ICAM-1 and VCAM-1 are shown in ( f ) with the quantification of mean intensity in ( g , h ). Data are presented as the mean+-s.e.m. from at least three independent experiments. * P <0.05 and ** P <0.01; two-way ANOVA followed by Bonferroni post-test. MAEC, mouse aortic endothelial cell.

CD106 (VCAM-1) Antibody in Immunohistochemistry (IHC) — Fig. 4 GRP78 in hepatocytes. Immunocyte-fluorescent study of GRP78 was done for primary murine hepatocytes after incubation for 24 h in either media alone (CNT group), media containing 0.2 mM palmitate (FFA group), or media containing 0.2 mM palmitate and 3 muM dantrolene (FFA + DAN group). A: Representative images of immunocyte-fluorescent study for GRP78 hepatocytes. B: GRP78 fluorescent intensity were calculated. There were significant differences between FFA group and FFA + DAN group. Values are the mean +- SEM of 60 cells from three different experiments. Fig. 4

CD106 (VCAM-1) Antibody in Flow Cytometry (Flow) — Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1061-82) in Flow Cytometry

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.25 µg/test

View 13 publications 13 publications

Functional Assay (FN)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

429

Conjugate

Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_466559

Product Specific Information

Description: The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin a4b7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported: The 429 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 429 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.