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Invitrogen
CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), eBioscience™
FIGURE: 1 / 31
CD107a (LAMP-1) Antibody (14-1079-80) in WB
Normal human peripheral blood cells were lysed and 10 µg of total protein per lane was immunoblotted using 1 µg/mL of Anti-Human CD107a (LAMP-1) Purified and revealed with Anti-Mouse HRP (left). Immunocytochemistry of HeLa cells stained with 5 µg/mL Anti-Human CD107a (LAMP-1) Purified followed by F (ab')2 Anti-Mouse IgG eFluor® 570 (center). Nuclei are stained with DAPI.
Product Details
14-1079-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Cynomolgus monkey,
Human,
Non-human primate
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
eBioH4A3
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
RRID
AB_467426
Product Specific Information
Description: The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated protein of approximately 110kDa. It is predominantly expressed intracellularly in the lysosomal/endosomal membrane in nearly all cells. CD107a is transiently expressed on the cell surface of degranulating cytolytic T cells, and is also upregulated on the surface of activated platelets and some cancer cells.
Applications Reported: Purified anti-human CD107a (LAMP-1) has been reported for use in flow cytometric analysis, immunohistochemistry, immunocytochemistry, and immunoblotting. It has also been reported for use in surface staining in a flow cytometric based degranulation assay. (Fluorochrome conjugated eBioH4B4 (H4B4) is recommended for use in flow cytometry.).
Applications Tested: This eBioH4A3 antibody has been tested by intracellular staining and flow cytometric analysis, immunocytochemistry of methanol-fixed cells, immunohistochemistry of formalin-fixed paraffin embedded human tissue using high or low pH antigen retrieval, or western blotting. For flow cytometry this can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For both immunocytochemistry and immunohistochemistry, this can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer's Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD107 antigen-like family member A; CD107a; LAMP-1; lysosomal-associated membrane protein 1; Lysosome-associated membrane glycoprotein 1; lysosome-associated membrane protein 1
Gene Aliases: CD107a; LAMP1; LAMPA; LGP120
UniProt ID: (Human) P11279
Entrez Gene ID: (Human) 3916
Molecular Function:
membrane trafficking regulatory protein
autophagy
granzyme-mediated apoptotic signaling pathway
positive regulation of natural killer cell degranulation
positive regulation of natural killer cell mediated cytotoxicity
viral entry into host cell
autophagic cell death
protein stabilization
establishment of protein localization to organelle
Golgi to lysosome transport
regulation of organelle transport along microtubule
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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