Invitrogen

CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), eBioscience™

Product Image Gallery

CD107a (LAMP-1) Antibody in Western Blot (WB) — Normal human peripheral blood cells were lysed and 10 µg of total protein per lane was immunoblotted using 1 µg/mL of Anti-Human CD107a (LAMP-1) Purified and revealed with Anti-Mouse HRP (left). Immunocytochemistry of HeLa cells stained with 5 µg/mL Anti-Human CD107a (LAMP-1) Purified followed by F (ab')2 Anti-Mouse IgG eFluor® 570 (center). Nuclei are stained with DAPI.

CD107a (LAMP-1) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of human formalin-fixed paraffin embedded kidney (right) stained with 5 µg/mL Anti-Human CD107a (LAMP-1) Purified followed by Anti-Mouse IgG Biotin, Avidin HRP followed by DAB visualization. Nuclei are counterstained with hematoxylin.

CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC) — Immunocytochemistry of HeLa cells stained with 5 µg/mL Anti-Human CD107a (LAMP-1) Purified followed by F (ab')2 Anti-Mouse IgG eFluor® 570 (left). Nuclei are stained with DAPI.

CD107a (LAMP-1) Antibody — Fig. 1 CD46 co-stimulation provides superior support for CTL activity. a CD46 expression on the surface of resting human CD4 + and CD8 + T cells assessed by FACS analysis ( n = 4, gating strategy in Supplementary Fig. 7a ). b Comparison of IFN-gamma, TNF-alpha and IL-10 secretion by CD3 + CD46-activated T cells. Purified CD4 + and CD8 + T cells from healthy donors were left non-activated (NA) or stimulated with immobilized antibodies to CD3, CD3 + CD28 or CD3 + CD46 and cytokines measured 60 h post activation ( n = 5). c, d Degranulation (CD107a staining, ( c )) and granzyme B expression ( d ) by CD8 + T cells upon CD46 co-stimulation. CD8 + T cells were stimulated as in ( a ) and CD107a and granzyme B expression assessed with left panels showing representative cytometry images and right panels corresponding quantifications ( n = 3, gating strategy in Supplementary Fig. 7b ). e Killing activity of CD46-activated CD8 + T cells. T cells were stimulated as depicted for 24 h and cytotoxic activity of differently activated CD8 + T cells towards DU145 target cells assessed 24 h post co-culture of T cells and DU145 cells ( n = 4, gating strategy in Supplementary Fig. 7c ). f Effect of CD46 co-stimulation on CD8 + T-cell proliferation. Purified T cells were activated as indicated for 5 days and cell proliferation measured each day ( n = 4) (black circles, non-activated cells; green, blue, and red circles, CD3, CD3 + CD28 or CD3 + CD46-activated cells, res

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/test

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunocytochemistry (ICC)

5 µg/mL

Immunofluorescence (IF)

5 µg/mL

View 6 publications 6 publications

Flow Cytometry (Flow)

0.5 µg/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human

Published species

Artificial Control, Cynomolgus Monkey, Human, Non-human primate

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

eBioH4A3

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_467426

Product Specific Information

Description: The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated protein of approximately 110kDa. It is predominantly expressed intracellularly in the lysosomal/endosomal membrane in nearly all cells. CD107a is transiently expressed on the cell surface of degranulating cytolytic T cells, and is also upregulated on the surface of activated platelets and some cancer cells.

Applications Reported: Purified anti-human CD107a (LAMP-1) has been reported for use in flow cytometric analysis, immunohistochemistry, immunocytochemistry, and immunoblotting. It has also been reported for use in surface staining in a flow cytometric based degranulation assay. (Fluorochrome conjugated eBioH4B4 (H4B4) is recommended for use in flow cytometry.).

Applications Tested: This eBioH4A3 antibody has been tested by intracellular staining and flow cytometric analysis, immunocytochemistry of methanol-fixed cells, immunohistochemistry of formalin-fixed paraffin embedded human tissue using high or low pH antigen retrieval, or western blotting. For flow cytometry this can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For both immunocytochemistry and immunohistochemistry, this can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer's Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD107 antigen-like family member A; CD107a; LAMP-1; lysosomal-associated membrane protein 1; Lysosome-associated membrane glycoprotein 1; lysosome-associated membrane protein 1

Gene Aliases: CD107a; LAMP1; LAMPA; LGP120

UniProt ID: (Human) P11279

Entrez Gene ID: (Human) 3916

Molecular Function: membrane traffic protein membrane trafficking regulatory protein