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      View (31) other CSF1R (CD115) antibodies

      Invitrogen

      CD115 (c-fms) Monoclonal Antibody (AFS98), PE, eBioscience™

      Published Figures 2 Published Figures
      References Icon 39 References
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      Catalog # 12-1152-81

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      Product Details

      TESTED APPLICATIONS DILUTION
      Flow Cytometry (Flow) 0.06 µg/test
      PUBLISHED APPLICATIONS
      Flow Cytometry (Flow) See 37 publications below
      Immunofluorescence (IF) See 1 publications below
      Western Blot (WB) See 1 publications below
      Species reactivity Mouse
      Published species Mouse
      Host / Isotype Rat / IgG2a, kappa
      Recommended Isotype Control Rat IgG2a kappa Isotype Control, PE, eBioscience™
      Class Monoclonal
      Type Antibody
      Clone AFS98
      Conjugate PE
      Excitation/Emission Profile View spectra
      Form Liquid
      Concentration 0.2 mg/mL
      Purification Affinity chromatography
      Storage buffer PBS, pH 7.2, with 0.1% gelatin
      Contains 0.09% sodium azide
      Storage conditions 4° C, store in dark, DO NOT FREEZE!
      RRID AB_465807

      Product Specific Information

      Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

      Applications Reported: The AFS98 antibody has been reported for use in flow cytometric analysis.

      Applications Tested: The AFS98 antibody has been tested by flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

      Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

      Filtration: 0.2 µm post-manufacturing filtered.

      Background/Target Information

      The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References (39)

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      Bioinformatics

      Protein Aliases: c-fms; CD115; CSF-1 receptor; CSF-1-R; Macrophage colony-stimulating factor 1 receptor; Proto-oncogene c-Fms; proto-oncogene fms

      Gene Aliases: AI323359; CD115; CSF-1R; Csf1r; Csfmr; Fim-2; Fms; M-CSF-R; M-CSFR

      UniProt ID: (Mouse) P09581

      Entrez Gene ID: (Mouse) 12978

      Function(s) Blue Arrow Icon
      positive regulation of protein phosphorylation immune system process protein phosphorylation inflammatory response transmembrane receptor protein tyrosine kinase signaling pathway cell proliferation positive regulation of cell proliferation regulation of cell shape phosphorylation peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway hemopoiesis osteoclast differentiation positive regulation of cell migration ruffle organization cellular response to macrophage colony-stimulating factor stimulus macrophage colony-stimulating factor signaling pathway positive regulation of tyrosine phosphorylation of Stat3 protein innate immune response regulation of bone resorption cell-cell junction maintenance positive regulation of osteoclast differentiation phosphatidylinositol metabolic process protein autophosphorylation phosphatidylinositol-mediated signaling positive regulation of protein tyrosine kinase activity positive regulation of ERK1 and ERK2 cascade cellular response to cytokine stimulus positive regulation of protein serine/threonine kinase activity positive regulation of chemokine secretion positive regulation of cell motility regulation of actin cytoskeleton reorganization
      Process(es) Blue Arrow Icon
      nucleotide binding
       protein kinase activity
       protein tyrosine kinase activity
       transmembrane receptor protein tyrosine kinase activity
       macrophage colony-stimulating factor receptor activity
       protein binding
       ATP binding
       kinase activity
       transferase activity
       transferase activity, transferring phosphorus-containing groups
       protein phosphatase binding
       cytokine binding
       protein homodimerization activity

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