Invitrogen

CD115 (c-fms) Monoclonal Antibody (AFS98), Functional Grade, eBioscience™

CD115 (c-fms) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 32

CD115 (c-fms) Antibody (16-1152-82) in Flow

Surface staining of thioglycolate-induced peritoneal exudate cells (PECs) with Anti-Mouse CD115 (c-fms) PE. Appropriate isotype controls were used (open histogram). Cells in the large scatter population were used for analysis.

Product Image Gallery

CD115 (c-fms) Antibody in Immunocytochemistry (ICC/IF) — Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Immunocytochemistry

CD115 (c-fms) Antibody in Immunohistochemistry (IHC) — Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Immunohistochemistry

Product Details

16-1152-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 25 publications 25 publications

Neutralization (Neu)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (FN)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AFS98

Conjugate

Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

RRID

AB_469046

Product Specific Information

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Applications Reported: The AFS98 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of ligand binding.

Applications Tested: The AFS98 antibody has been tested by blocking of fluorochrome conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) in mesenchymal stem cells leads to osteopenia by impairing bone remodeling.

The Journal of biological chemistry

Yan Q,Gao H,Yao Q,Ling K,Xiao G

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling.

The EMBO journal

Wieghofer P,Hagemeyer N,Sankowski R,Schlecht A,Staszewski O,Amann L,Gruber M,Koch J,Hausmann A,Zhang P,Boneva S,Masuda T,Hilgendorf I,Goldmann T,Böttcher C,Priller J,Rossi FM,Lange C,Prinz M

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Mon Mar 15 00:00:00 EDT 2021

Low-dose 2-deoxy glucose stabilises tolerogenic dendritic cells and generates potent in vivo immunosuppressive effects.

Cellular and molecular life sciences : CMLS

Christofi M,Le Sommer S,Mölzer C,Klaska IP,Kuffova L,Forrester JV

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

HOXA11 plays critical roles in disease progression and response to cytarabine in AML.

Oncology reports

Fu JF,Shih LY,Yen TH

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

CX3CR1 But Not CCR2 Expression Is Required for the Development of Autoimmune Peripheral Neuropathy in Mice.

Frontiers in immunology

Oladiran O,Shi XQ,Fournier S,Zhang J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Tue Dec 21 00:00:00 EST 2021

Inhibition of TLR4 signaling protects mice from sensory and motor dysfunction in an animal model of autoimmune peripheral neuropathy.

Journal of neuroinflammation

Oladiran O,Shi XQ,Yang M,Fournier S,Zhang J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Mon Mar 22 00:00:00 EDT 2021

Inflammation rapidly recruits mammalian GMP and MDP from bone marrow into regional lymphatics.

eLife

Serrano-Lopez J,Hegde S,Kumar S,Serrano J,Fang J,Wellendorf AM,Roche PA,Rangel Y,Carrington LJ,Geiger H,Grimes HL,Luther S,Maillard I,Sanchez-Garcia J,Starczynowski DT,Cancelas JA

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Thu Apr 08 00:00:00 EDT 2021

Radiation causes tissue damage by dysregulating inflammasome-gasdermin D signaling in both host and transplanted cells.

PLoS biology

Xiao J,Wang C,Yao JC,Alippe Y,Yang T,Kress D,Sun K,Kostecki KL,Monahan JB,Veis DJ,Abu-Amer Y,Link DC,Mbalaviele G

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Antibiotic-induced dysbiosis of gut microbiota impairs corneal development in postnatal mice by affecting CCR2 negative macrophage distribution.

Mucosal immunology

Wu M,Liu J,Li F,Huang S,He J,Xue Y,Fu T,Feng S,Li Z

16-1152-82 was used in Functional assay to propose that the CCR2- macrophage population is a crucial mediator through which gut microbiota affect corneal development in postnatal mice.

Wed Jan 01 00:00:00 EST 2020

Temporospatial distribution and transcriptional profile of retinal microglia in the oxygen-induced retinopathy mouse model.

Glia

Boeck M,Thien A,Wolf J,Hagemeyer N,Laich Y,Yusuf D,Backofen R,Zhang P,Boneva S,Stahl A,Hilgendorf I,Agostini H,Prinz M,Wieghofer P,Schlunck G,Schlecht A,Lange C

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Androgen Receptor Signaling Positively Regulates Monocytic Development.

Frontiers in immunology

Consiglio CR,Gollnick SO

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Thu Apr 29 00:00:00 EDT 2021

The Innate Immune Response to Infection Induces Erythropoietin-Dependent Replenishment of the Dendritic Cell Compartment.

Frontiers in immunology

Einwächter H,Heiseke A,Schlitzer A,Gasteiger G,Adler H,Voehringer D,Manz MG,Ruzsics Z,Dölken L,Koszinowski UH,Sparwasser T,Reindl W,Jordan S

16-1152-82 was used in Flow Cytometry to investigate the molecular and cellular mechanisms of inflammation-induced dendritic cell generation.

Mon Apr 19 00:00:00 EDT 2021

Concomitant type I IFN and M-CSF signaling reprograms monocyte differentiation and drives pro-tumoral arginase production.

EBioMedicine

Tong Y,Zhou L,Yang L,Guo P,Cao Y,Qin FX,Liu J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Chemotherapy Induces Breast Cancer Stemness in Association with Dysregulated Monocytosis.

Clinical cancer research : an official journal of the American Association for Cancer Research

Liu L,Yang L,Yan W,Zhai J,Pizzo DP,Chu P,Chin AR,Shen M,Dong C,Ruan X,Ren X,Somlo G,Wang SE

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Tue May 15 00:00:00 EDT 2018

Bone loss in C57BL/6J-OlaHsd mice, a substrain of C57BL/6J carrying mutated alpha-synuclein and multimerin-1 genes.

Journal of cellular physiology

Liron T,Raphael B,Hiram-Bab S,Bab IA,Gabet Y

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Antibiotic-Induced Dysbiosis of Gut Microbiota Impairs Corneal Nerve Regeneration by Affecting CCR2-Negative Macrophage Distribution.

The American journal of pathology

Liu J,Wu M,He J,Xiao C,Xue Y,Fu T,Lin C,Dong D,Li Z

16-1152 was used in Immunodepletion to study the relationship between dysbiosis of the gut microbiota induced by antibiotic treatment and impaired corneal nerve regeneration.

Sat Dec 01 00:00:00 EST 2018

Captopril mitigates splenomegaly and myelofibrosis in the Gata1^low murine model of myelofibrosis.

Journal of cellular and molecular medicine

Corey SJ,Jha J,McCart EA,Rittase WB,George J,Mattapallil JJ,Mehta H,Ognoon M,Bylicky MA,Summers TA,Day RM

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Neutrophil and Macrophage Cell Surface Colony-Stimulating Factor 1 Shed by ADAM17 Drives Mouse Macrophage Proliferation in Acute and Chronic Inflammation.

Molecular and cellular biology

Tang J,Frey JM,Wilson CL,Moncada-Pazos A,Levet C,Freeman M,Rosenfeld ME,Stanley ER,Raines EW,Bornfeldt KE

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Advanced Glycation End Products Enhance Murine Monocyte Proliferation in Bone Marrow and Prime Them into an Inflammatory Phenotype through MAPK Signaling.

Journal of diabetes research

Jin X,Liu L,Zhang Y,Xiang Y,Yin G,Lu Y,Shi L,Dong J,Shen C

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Wed Oct 10 00:00:00 EDT 2018

Dietary PUFAs attenuate NLRP3 inflammasome activation via enhancing macrophage autophagy.

Journal of lipid research

Shen L,Yang Y,Ou T,Key CC,Tong SH,Sequeira RC,Nelson JM,Nie Y,Wang Z,Boudyguina E,Shewale SV,Zhu X

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Erythropoietin enhances Kupffer cell number and activity in the challenged liver.

Scientific reports

Gilboa D,Haim-Ohana Y,Deshet-Unger N,Ben-Califa N,Hiram-Bab S,Reuveni D,Zigmond E,Gassmann M,Gabet Y,Varol C,Neumann D

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Mon Sep 04 00:00:00 EDT 2017

Leukaemogenic effects of Ptpn11 activating mutations in the stem cell microenvironment.

Nature

Dong L,Yu WM,Zheng H,Loh ML,Bunting ST,Pauly M,Huang G,Zhou M,Broxmeyer HE,Scadden DT,Qu CK

16115282 was used in immunohistochemistry - frozen section to elucidate the contribution of Ptpn11 mutations in the bone marrow microenvironment to leukaemogenesis

Thu Nov 10 00:00:00 EST 2016

Enforced Expression of Hoxa3 Inhibits Classical and Promotes Alternative Activation of Macrophages In Vitro and In Vivo.

Journal of immunology (Baltimore, Md. : 1950)

Al Sadoun H,Burgess M,Hentges KE,Mace KA

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice.

Cell death & disease

Wang T,Wang Z,Yang P,Xia L,Zhou M,Wang S,Du J,Zhang J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Thu Apr 07 00:00:00 EDT 2016

miR-126 and miR-126* repress recruitment of mesenchymal stem cells and inflammatory monocytes to inhibit breast cancer metastasis.

Nature cell biology

Zhang Y,Yang P,Sun T,Li D,Xu X,Rui Y,Li C,Chong M,Ibrahim T,Mercatali L,Amadori D,Lu X,Xie D,Li QJ,Wang XF

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

Migration of bone marrow-derived cells into the central nervous system in models of neurodegeneration.

The Journal of comparative neurology

Lampron A,Pimentel-Coelho PM,Rivest S

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2013

Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages.

Blood

He H,Xu J,Warren CM,Duan D,Li X,Wu L,Iruela-Arispe ML

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Immunofluorescence

Thu Oct 11 00:00:00 EDT 2012

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

Stat3-dependent acute Rantes production in vascular smooth muscle cells modulates inflammation following arterial injury in mice.

The Journal of clinical investigation

Kovacic JC,Gupta R,Lee AC,Ma M,Fang F,Tolbert CN,Walts AD,Beltran LE,San H,Chen G,St Hilaire C,Boehm M

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Immunofluorescence

Fri Jan 01 00:00:00 EST 2010

Phospholipase Cgamma2 mediates RANKL-stimulated lymph node organogenesis and osteoclastogenesis.

The Journal of biological chemistry

Chen Y,Wang X,Di L,Fu G,Chen Y,Bai L,Liu J,Feng X,McDonald JM,Michalek S,He Y,Yu M,Fu YX,Wen R,Wu H,Wang D

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Fri Oct 24 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: c-fms; CD115; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; kinase CSFR; Macrophage colony-stimulating factor 1 receptor; OTTHUMP00000224093; Proto-oncogene c-Fms; proto-oncogene fms

Gene Aliases: AI323359; CD115; CSF-1R; Csf1r; Csfmr; Fim-2; Fms; M-CSF-R; M-CSFR

UniProt ID: (Mouse) P09581

Entrez Gene ID: (Mouse) 12978

Molecular Function: transmembrane signal receptor

Function(s)

Process(es)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity macrophage colony-stimulating factor receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein phosphatase binding cytokine binding protein homodimerization activity

CD115 (c-fms) Monoclonal Antibody (AFS98), Functional Grade, eBioscience™

CD115 (c-fms) Antibody (16-1152-82) in Flow

Product Details

16-1152-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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