Invitrogen

CD115 (c-fms) Monoclonal Antibody (AFS98), Functional Grade, eBioscience™

Product Image Gallery

CD115 (c-fms) Antibody in Flow Cytometry (Flow) — Surface staining of thioglycolate-induced peritoneal exudate cells (PECs) with Anti-Mouse CD115 (c-fms) PE. Appropriate isotype controls were used (open histogram). Cells in the large scatter population were used for analysis.

CD115 (c-fms) Antibody in Immunocytochemistry (ICC/IF) — Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Immunocytochemistry

CD115 (c-fms) Antibody in Immunohistochemistry (IHC) — Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Immunohistochemistry

Product Details

16-1152-82

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	1 µg/test	View 13 publications 13 publications
Neutralization (Neu)	Assay-Dependent	View 1 publication 1 publication
Functional Assay (FN)	Assay-Dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Fish, Human, Mouse
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	AFS98
Conjugate	Functional Grade Alexa Fluor 488 APC APC-eFluor 780 Biotin PE PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	no preservative
Storage conditions	4° C
RRID	AB_469046

Product Specific Information

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Applications Reported: The AFS98 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of ligand binding.

Applications Tested: The AFS98 antibody has been tested by blocking of fluorochrome conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CSF1R (colony stimulating factor 1 receptor) is a cytokine that controls the production, differentiation, and function of macrophages. CSF1R mediates most if not all of the biological effects of CSF1. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Androgen Receptor Signaling Positively Regulates Monocytic Development.

Frontiers in immunology

Consiglio CR,Gollnick SO

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Thu Apr 29 00:00:00 EDT 2021

Antibiotic-induced dysbiosis of gut microbiota impairs corneal development in postnatal mice by affecting CCR2 negative macrophage distribution.

Mucosal immunology

Wu M,Liu J,Li F,Huang S,He J,Xue Y,Fu T,Feng S,Li Z

16-1152-82 was used in Functional assay to propose that the CCR2- macrophage population is a crucial mediator through which gut microbiota affect corneal development in postnatal mice.

Wed Jan 01 00:00:00 EST 2020

Antibiotic-Induced Dysbiosis of Gut Microbiota Impairs Corneal Nerve Regeneration by Affecting CCR2-Negative Macrophage Distribution.

The American journal of pathology

Liu J,Wu M,He J,Xiao C,Xue Y,Fu T,Lin C,Dong D,Li Z

16-1152 was used in Immunodepletion to study the relationship between dysbiosis of the gut microbiota induced by antibiotic treatment and impaired corneal nerve regeneration.

Sat Dec 01 00:00:00 EST 2018

Advanced Glycation End Products Enhance Murine Monocyte Proliferation in Bone Marrow and Prime Them into an Inflammatory Phenotype through MAPK Signaling.

Journal of diabetes research

Jin X,Liu L,Zhang Y,Xiang Y,Yin G,Lu Y,Shi L,Dong J,Shen C

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Wed Oct 10 00:00:00 EDT 2018

Neutrophil and Macrophage Cell Surface Colony-Stimulating Factor 1 Shed by ADAM17 Drives Mouse Macrophage Proliferation in Acute and Chronic Inflammation.

Molecular and cellular biology

Tang J,Frey JM,Wilson CL,Moncada-Pazos A,Levet C,Freeman M,Rosenfeld ME,Stanley ER,Raines EW,Bornfeldt KE

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Chemotherapy Induces Breast Cancer Stemness in Association with Dysregulated Monocytosis.

Clinical cancer research : an official journal of the American Association for Cancer Research

Liu L,Yang L,Yan W,Zhai J,Pizzo DP,Chu P,Chin AR,Shen M,Dong C,Ruan X,Ren X,Somlo G,Wang SE

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Tue May 15 00:00:00 EDT 2018

Bone loss in C57BL/6J-OlaHsd mice, a substrain of C57BL/6J carrying mutated alpha-synuclein and multimerin-1 genes.

Journal of cellular physiology

Liron T,Raphael B,Hiram-Bab S,Bab IA,Gabet Y

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Erythropoietin enhances Kupffer cell number and activity in the challenged liver.

Scientific reports

Gilboa D,Haim-Ohana Y,Deshet-Unger N,Ben-Califa N,Hiram-Bab S,Reuveni D,Zigmond E,Gassmann M,Gabet Y,Varol C,Neumann D

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Mon Sep 04 00:00:00 EDT 2017

Dietary PUFAs attenuate NLRP3 inflammasome activation via enhancing macrophage autophagy.

Journal of lipid research

Shen L,Yang Y,Ou T,Key CC,Tong SH,Sequeira RC,Nelson JM,Nie Y,Wang Z,Boudyguina E,Shewale SV,Zhu X

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Leukaemogenic effects of Ptpn11 activating mutations in the stem cell microenvironment.

Nature

Dong L,Yu WM,Zheng H,Loh ML,Bunting ST,Pauly M,Huang G,Zhou M,Broxmeyer HE,Scadden DT,Qu CK

16115282 was used in immunohistochemistry - frozen section to elucidate the contribution of Ptpn11 mutations in the bone marrow microenvironment to leukaemogenesis

Thu Nov 10 00:00:00 EST 2016

Enforced Expression of Hoxa3 Inhibits Classical and Promotes Alternative Activation of Macrophages In Vitro and In Vivo.

Journal of immunology (Baltimore, Md. : 1950)

Al Sadoun H,Burgess M,Hentges KE,Mace KA

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice.

Cell death & disease

Wang T,Wang Z,Yang P,Xia L,Zhou M,Wang S,Du J,Zhang J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Thu Apr 07 00:00:00 EDT 2016

Migration of bone marrow-derived cells into the central nervous system in models of neurodegeneration.

The Journal of comparative neurology

Lampron A,Pimentel-Coelho PM,Rivest S

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2013

miR-126 and miR-126* repress recruitment of mesenchymal stem cells and inflammatory monocytes to inhibit breast cancer metastasis.

Nature cell biology

Zhang Y,Yang P,Sun T,Li D,Xu X,Rui Y,Li C,Chong M,Ibrahim T,Mercatali L,Amadori D,Lu X,Xie D,Li QJ,Wang XF

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages.

Blood

He H,Xu J,Warren CM,Duan D,Li X,Wu L,Iruela-Arispe ML

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Immunofluorescence

Thu Oct 11 00:00:00 EDT 2012

Stat3-dependent acute Rantes production in vascular smooth muscle cells modulates inflammation following arterial injury in mice.

The Journal of clinical investigation

Kovacic JC,Gupta R,Lee AC,Ma M,Fang F,Tolbert CN,Walts AD,Beltran LE,San H,Chen G,St Hilaire C,Boehm M

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Immunofluorescence

Fri Jan 01 00:00:00 EST 2010

Phospholipase Cgamma2 mediates RANKL-stimulated lymph node organogenesis and osteoclastogenesis.

The Journal of biological chemistry

Chen Y,Wang X,Di L,Fu G,Chen Y,Bai L,Liu J,Feng X,McDonald JM,Michalek S,He Y,Yu M,Fu YX,Wen R,Wu H,Wang D

Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry

Fri Oct 24 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: c-fms; CD115; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; kinase CSFR; Macrophage colony-stimulating factor 1 receptor; OTTHUMP00000224093; Proto-oncogene c-Fms; proto-oncogene fms

Gene Aliases: AI323359; CD115; CSF-1R; Csf1r; Csfmr; Fim-2; Fms; M-CSF-R; M-CSFR

UniProt ID: (Mouse) P09581

Entrez Gene ID: (Mouse) 12978

Molecular Function: transmembrane signal receptor