Invitrogen

CD115 (c-fms) Monoclonal Antibody (AFS98), Functional Grade, eBioscience™

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CD115 (c-fms) Antibody — Figure 1 AGEs increased proportions of CD115 + monocytes and their inflammatory subset in the bone marrow. Mice were divided into control ( n = 11) and AGE ( n = 11) groups. The AGE group received AGEs (25 mg/kg) via intraperitoneal injection once a day for 7 consecutive days, while the control group received BSA. On the 8th day, 5 mice in each group were sacrificed and bone marrow mononuclear cells were isolated for surface marker analysis by flow cytometry. The other 6 mice in each group also were sacrificed, and bone marrow CD115 + monocytes were sorted for real-time PCR analysis. (a) Proportions of CD115 + monocytes in the bone marrow. Bar graphs represent the results (mean +- SD) of five independent experiments. (b) Proportions of CD115 + Ly6C high inflammatory monocytes in the bone marrow. Bar graphs represent the results (mean +- SD) of five independent experiments. (c) mRNA expression of IL-1 beta , TNF- alpha , and IL-10 in CD115 + monocytes. Bar graphs represent the results (mean +- SD) of three independent experiments. * P < 0.05 compared to the control group.

CD115 (c-fms) Antibody in Flow Cytometry (Flow) — Surface staining of thioglycolate-induced peritoneal exudate cells (PECs) with Anti-Mouse CD115 (c-fms) PE. Appropriate isotype controls were used (open histogram). Cells in the large scatter population were used for analysis.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1 µg/test

Functional Assay (FN)

Assay-Dependent

Neutralization (Neu)

Assay-Dependent

Published Applications

Flow Cytometry (Flow)

See 8 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 1 publication below

Immunofluorescence (IF)

See 2 publications below

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host / Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AFS98

Conjugate

Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

RRID

AB_469046

Product Specific Information

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Applications Reported: The AFS98 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of ligand binding.

Applications Tested: The AFS98 antibody has been tested by blocking of fluorochrome conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-FMS; CD115; CSF-1 receptor; CSF-1-R; CSFR; FIM2; FMS; Macrophage colony-stimulating factor 1 receptor; Proto-oncogene c-Fms; proto-oncogene fms

Gene Aliases: AI323359; CD115; CSF-1R; Csf1r; Csfmr; Fim-2; Fms; M-CSF-R; M-CSFR

UniProt ID: (Mouse) P09581

Entrez Gene ID: (Mouse) 12978

Function(s)

Process(es)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity macrophage colony-stimulating factor receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein phosphatase binding cytokine binding protein homodimerization activity

CD115 (c-fms) Monoclonal Antibody (AFS98), Functional Grade, eBioscience™

Product Details

Product Specific Information

Target Information

Bioinformatics

Material safety data sheets (MSDS)

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