Invitrogen

CD115 (c-fms) Monoclonal Antibody (AFS98), Alexa Fluor™ 488, eBioscience™

Product Image Gallery

CD115 (c-fms) Antibody in Flow Cytometry (Flow) — Staining of thioglycolate-induced peritoneal exudeate cells (PECs) with 0.25 µg of Rat IgG2a kappa Isotype Control Alexa Fluor® 488 (Product # 53-4321-80) (blue histogram) or 0.25 µg of Anti-Mouse CD115 (c-fms) Alexa Fluor® 488 (purple histogram). Total viable cells were used for analysis.

CD115 (c-fms) Antibody in Immunocytochemistry (ICC/IF) — Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Immunocytochemistry

CD115 (c-fms) Antibody in Immunohistochemistry (IHC) — Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Immunohistochemistry

Product Details

53-1152-82

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	0.5 µg/test	View 16 publications 16 publications

Product Specifications
Species Reactivity	Mouse
Published species	Fish, Human, Mouse
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	AFS98
Conjugate	Alexa Fluor™ 488 APC APC-eFluor 780 Biotin Functional Grade PE PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2016696

Product Specific Information

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AFS98 antibody has been tested by flow cytometric analysis of mouse peritoneal elicited cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10e5 to 10e8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CSF1R (colony stimulating factor 1 receptor) is a cytokine that controls the production, differentiation, and function of macrophages. CSF1R mediates most if not all of the biological effects of CSF1. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Androgen Receptor Signaling Positively Regulates Monocytic Development.

Frontiers in immunology

Consiglio CR,Gollnick SO

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Thu Apr 29 00:00:00 EDT 2021

Inhibition of TLR4 signaling protects mice from sensory and motor dysfunction in an animal model of autoimmune peripheral neuropathy.

Journal of neuroinflammation

Oladiran O,Shi XQ,Yang M,Fournier S,Zhang J

53-1152 was used in Flow cytometry/Cell sorting to identify the critical contribution of TLR4-mediated macrophage activation in disease course and provided strong evidence to support TLR4 as a useful drug target for treating inflammatory autoimmune neuropathy.

Mon Mar 22 00:00:00 EDT 2021

Inflammasome Activation Triggers Blood Clotting and Host Death through Pyroptosis.

Immunity

Wu C,Lu W,Zhang Y,Zhang G,Shi X,Hisada Y,Grover SP,Zhang X,Li L,Xiang B,Shi J,Li XA,Daugherty A,Smyth SS,Kirchhofer D,Shiroishi T,Shao F,Mackman N,Wei Y,Li Z

53-1152-82 was used in Flow Cytometry to identify inflammasome activation as a trigger for blood clotting through pyroptosis.

Tue Jun 18 00:00:00 EDT 2019

Advanced Glycation End Products Enhance Murine Monocyte Proliferation in Bone Marrow and Prime Them into an Inflammatory Phenotype through MAPK Signaling.

Journal of diabetes research

Jin X,Liu L,Zhang Y,Xiang Y,Yin G,Lu Y,Shi L,Dong J,Shen C

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Wed Oct 10 00:00:00 EDT 2018

Neutrophil and Macrophage Cell Surface Colony-Stimulating Factor 1 Shed by ADAM17 Drives Mouse Macrophage Proliferation in Acute and Chronic Inflammation.

Molecular and cellular biology

Tang J,Frey JM,Wilson CL,Moncada-Pazos A,Levet C,Freeman M,Rosenfeld ME,Stanley ER,Raines EW,Bornfeldt KE

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Bone loss in C57BL/6J-OlaHsd mice, a substrain of C57BL/6J carrying mutated alpha-synuclein and multimerin-1 genes.

Journal of cellular physiology

Liron T,Raphael B,Hiram-Bab S,Bab IA,Gabet Y

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Erythropoietin enhances Kupffer cell number and activity in the challenged liver.

Scientific reports

Gilboa D,Haim-Ohana Y,Deshet-Unger N,Ben-Califa N,Hiram-Bab S,Reuveni D,Zigmond E,Gassmann M,Gabet Y,Varol C,Neumann D

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Mon Sep 04 00:00:00 EDT 2017

Enforced Expression of Hoxa3 Inhibits Classical and Promotes Alternative Activation of Macrophages In Vitro and In Vivo.

Journal of immunology (Baltimore, Md. : 1950)

Al Sadoun H,Burgess M,Hentges KE,Mace KA

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice.

Cell death & disease

Wang T,Wang Z,Yang P,Xia L,Zhou M,Wang S,Du J,Zhang J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Thu Apr 07 00:00:00 EDT 2016

Cellular changes in blood indicate severe respiratory disease during influenza infections in mice.

PloS one

Dengler L,Kühn N,Shin DL,Hatesuer B,Schughart K,Wilk E

53-1152 was used in Flow cytometry/Cell sorting to investigate the link between granulocyte to lymphocyte ratio in the blood and severe influenza disease in mice, to enable improvement of early diagnostic options of severe influenza disease.

Mon Jan 18 00:00:00 EST 2016

Spatiotemporal expression dynamics of selectins govern the sequential extravasation of neutrophils and monocytes in the acute inflammatory response.

Arteriosclerosis, thrombosis, and vascular biology

Zuchtriegel G,Uhl B,Hessenauer ME,Kurz AR,Rehberg M,Lauber K,Krombach F,Reichel CA

53-1152 was used in Flow cytometry/Cell sorting to provide novel insights into the mechanisms initiating the sequential infiltration of the perivascular tissue by neutrophils and monocytes in the acute inflammatory response.

Wed Apr 01 00:00:00 EDT 2015

Inhibition of Ca²⁺/calmodulin-dependent protein kinase kinase 2 stimulates osteoblast formation and inhibits osteoclast differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Cary RL,Waddell S,Racioppi L,Long F,Novack DV,Voor MJ,Sankar U

53-1152 was used in Flow cytometry/Cell sorting to reveal a novel function for CaMKK2 in bone remodelling and highlight the potential for therapeutic inhibition as a bone anabolic strategy.

Mon Jul 01 00:00:00 EDT 2013

miR-126 and miR-126* repress recruitment of mesenchymal stem cells and inflammatory monocytes to inhibit breast cancer metastasis.

Nature cell biology

Zhang Y,Yang P,Sun T,Li D,Xu X,Rui Y,Li C,Chong M,Ibrahim T,Mercatali L,Amadori D,Lu X,Xie D,Li QJ,Wang XF

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

MicroRNA-33 deficiency reduces the progression of atherosclerotic plaque in ApoE-/- mice.

Journal of the American Heart Association

Horie T,Baba O,Kuwabara Y,Chujo Y,Watanabe S,Kinoshita M,Horiguchi M,Nakamura T,Chonabayashi K,Hishizawa M,Hasegawa K,Kume N,Yokode M,Kita T,Kimura T,Ono K

53-1152 was used in Flow cytometry/Cell sorting to investigate the impact of the genetic loss of miR-33 in a mouse model of atherosclerosis.

Sat Dec 01 00:00:00 EST 2012

Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages.

Blood

He H,Xu J,Warren CM,Duan D,Li X,Wu L,Iruela-Arispe ML

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Immunofluorescence

Thu Oct 11 00:00:00 EDT 2012

Stat3-dependent acute Rantes production in vascular smooth muscle cells modulates inflammation following arterial injury in mice.

The Journal of clinical investigation

Kovacic JC,Gupta R,Lee AC,Ma M,Fang F,Tolbert CN,Walts AD,Beltran LE,San H,Chen G,St Hilaire C,Boehm M

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Immunofluorescence

Fri Jan 01 00:00:00 EST 2010

Phospholipase Cgamma2 mediates RANKL-stimulated lymph node organogenesis and osteoclastogenesis.

The Journal of biological chemistry

Chen Y,Wang X,Di L,Fu G,Chen Y,Bai L,Liu J,Feng X,McDonald JM,Michalek S,He Y,Yu M,Fu YX,Wen R,Wu H,Wang D

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Fri Oct 24 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: c-fms; CD115; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; kinase CSFR; Macrophage colony-stimulating factor 1 receptor; OTTHUMP00000224093; Proto-oncogene c-Fms; proto-oncogene fms

Gene Aliases: AI323359; CD115; CSF-1R; Csf1r; Csfmr; Fim-2; Fms; M-CSF-R; M-CSFR

UniProt ID: (Mouse) P09581

Entrez Gene ID: (Mouse) 12978

Molecular Function: transmembrane signal receptor