|Flow Cytometry (Flow)||5 µL (1 µg)/test|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, store in dark, DO NOT FREEZE!|
Description: The 4H1 monoclonal antibody reacts with the human CD116 molecule, the alpha subunit of GM-CSF receptor. The alpha subunit associates with the common beta chain (CD131) to form the high affinity receptor for GM-CSF. The GM-CSFR alpha chain is expressed by granulocytes, monocytes, endothelial cells, fibroblasts and some tumor cells.
Applications Reported: 4H1 has been reported for use in flow cytometric analysis.
Applications Tested: This 4H1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
The human IL-3, IL-5 and GM-CSF receptors are each composed of both unique a subunits and a common beta subunit. The a subunits are low affinity ligand binding proteins while the beta subunits do not themselves bind ligand, but are required for high affinity binding by the a subunits. In contrast, the mouse IL-3 receptor has two distinct beta subunits, one that functions only in IL-3 mediated cell signaling and a second that is shared with IL-5 and GM-CSF. The murine beta-subunits are 91% homologous at the amino acid level but only 56% homologous to the human beta subunit. Although neither the murine nor the human beta subunit contains tyrosine kinase domains, both activate tyrosine phosphorylation mediated signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD116; CD116 antigen; CDw116; colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage); GM-CSF receptor alpha subunit; GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; granulocyte-macrophage colony-stimulating factor receptor alpha chain; Granulocyte-macrophage colony-stimulating factor receptor subunit alpha
Gene Aliases: CD116; CDw116; CSF2R; CSF2RA; CSF2RAX; CSF2RAY; CSF2RX; CSF2RY; GM-CSF-R-alpha; GMCSFR; GMR; SMDP4
UniProt ID: (Human) P15509
Entrez Gene ID: (Human) 1438
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