Invitrogen

CD117 (c-Kit) Monoclonal Antibody (2B8), eBioscience™

40 Published Figures

82 References

Datasheet

Datasheet

Product Image Gallery

CD117 (c-Kit) Antibody in Flow Cytometry (Flow) — Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2b kappa Isotype Control PE (Product # 12-4031-82) (left) or 0.125 µg of Anti-Mouse CD117 (c-Kit) PE (right). Total viable cells were used for analysis.

CD117 (c-Kit) Antibody — Figure 1. Dual-color Bcl11b reporter strategy can reveal epigenetic mechanisms controlling T-cell lineage commitment. ( A ) Overview of early T-cell development. Bcl11b turns on to silence alternate fate potentials and drive T-cell fate commitment. ETP - early thymic progenitor; DN2 - CD4 - CD8 - double negative-2A progenitor; DP - CD4 + CD8 + ; NK - natural killer; DC - dendritic cell. ( B ) Dual-allelic Bcl11b reporter cells, where two distinguishable fluorescent proteins (YFP and mCherry) are inserted non-disruptively into the same sites on the two endogenous Bcl11b loci. ( C ) Flow cytometry plots show cKit versus CD25 levels in CD4 - CD8 - double negative (DN) thymic progenitors (left), along with Bcl11b-YFP versus Bcl11b-mCh expression levels in the indicated DN progenitor subsets from dual Bcl11b reporter mice. Arrowheads indicate cells expressing one copy of Bcl11b . ( D ) Flow plots show Bcl11b-YFP versus Bcl11b-mCh levels in CD4 + CD8 + double positive (DP) T-cell precursors from the thymus (left), or CD4 (center) or CD8 (right) T-cells from the spleen. Results are representative of analysis of 6-8 mice from two independent experiments. See also Figure 1--figure supplement 1 . Figure 1--figure supplement 1. Experimental strategy for generating different Bcl11b reporter mouse strains. The two Bcl11b loci were targeted in embryonic stem (ES) cells using homologous recombination, followed by drug selection using the indicated drug-res

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.5 µg/test

Immunoprecipitation (IP)

Assay-Dependent

Published Applications

Flow Cytometry (Flow)

See 65 publications below

Immunofluorescence (IF)

See 8 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 3 publications below

Immunohistochemistry (IHC)

See 3 publications below

Immunocytochemistry (ICC)

See 1 publication below

Miscellaneous PubMed (MISC)

See 1 publication below

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Mouse, Pig

Published species

Fish, Mouse

Host / Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

2B8

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_467433

Product Specific Information

Description: The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor, and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145-kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1 low) and negative for lineage markers. The interaction of the mouse c-Kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells.

Applications Reported: The 2B8 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested: This 2B8 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

c-Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c-Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 703 of c-Kit allows binding of Grb2 and activation of the Ras-Raf-ERK1 and 2 signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: belly-spot; C-Kit; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; piebald trait protein; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; spotted sterile male; Steel Factor Receptor; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Gene Aliases: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; MGF; SCO1; SCO5; SOW3; Ssm; Tr-kit; W

Entrez Gene ID: (Pig) 396810, (Mouse) 16590

Function(s)