Invitrogen

CD117 (c-Kit) Monoclonal Antibody (2B8), eBioscience™

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CD117 (c-Kit) Antibody in Flow Cytometry (Flow) — Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2b kappa Isotype Control PE (Product # 12-4031-82) (left) or 0.125 µg of Anti-Mouse CD117 (c-Kit) PE (right). Total viable cells were used for analysis.

CD117 (c-Kit) Antibody in Immunocytochemistry (ICC/IF) — Figure 3 CSF1R + EMPs are positive for several hematopoietic/endothelial cell markers but do not take up AcLDL. Confocal microscopy images of wild-type yolk sacs dissected at E9.0 (15 somite pairs) and immunostained for CSF1R and for hemogenic markers c-kit ( A , n = 3) and CD34 ( B , n = 3). White arrowheads indicate CSF1R + cells that co-express hemogenic/hematopoietic CD34 and c-kit. Though CSF1R + cells expressed most endothelial cell markers, they did not take up AcLDL as endothelial cells do ( C , green). Counterstaining with VE-Cadherin shows AcLDL + endothelial cells (blue). Embryos were then dissected at either E8.0 (4-6 somite pairs) or E8.5 (10-12 somite pairs), injected with AF488-AcLDL and cultured for 24 hours. Quantification of tdT + AcLDL + cells show that the majority of tdT + cells are also AcLDL + when the injection is done before EMPs arise, however only a third of tdT + cells are labelled if injected after EMPs arise ( D ). Embryos at E8.5 (8-10 somite pairs) were dissected and stained for hemogenic endothelium marker Runx1, endothelial marker PECAM-1, and AF594-AcLDL. Flow cytometry analysis shows that most Runx1 + cells at this stage are AcLDL + ( E ) and PECAM-1 positive ( F ). Scale bar: 20 mum. All values are mean +- SEM. ***p < 0.001; Two-tailed Student's t-test.

CD117 (c-Kit) Antibody in Immunohistochemistry (IHC) — Extended data figure 9 Bone marrow blood vessel types can be distinguished based upon vessel diameter, continuity of basal lamina, morphology, and position, and we could not detect any difference in the distribution of HSCs in the bone marrow of male and female mice a,b, Schematic (a) and properties (b) of blood vessels in the bone marrow. Blood enters the marrow through arterioles that branch as they become smaller in diameter and approach the endosteum, where they connect to smaller diameter transition zone capillaries near the bone surface. These transition zone capillaries connect to the large diameter sinusoids that feed blood into the central sinuous through which it leaves the bone marrow in venous circulation. c , Each type of blood vessel was distinguished based on vessel diameter, continuity of basal lamina, morphology, and position then color coded using published criteria 17 , 30 , 46 , 47 . To create distinct digital surfaces associated with each type of blood vessel we first designated all laminin-stained blood vessels in the outer 20% of the marrow volume (adjacent to the endosteum) as transition zone vessels (blue). Arterioles were identified and manually traced in the remaining 80% of marrow volume based on high intensity laminin staining, continuous basal lamina, and morphology. Remaining blood vessels with low intensity laminin staining, fenestrated basal lamina, large diameter, and sinusoidal morphology were designated sinusoids. The longitudinal images (t

CD117 (c-Kit) Antibody in Western Blot, Flow Cytometry (WB, Flow) — Published figure using CD117 (c-Kit) monoclonal antibody (Product # 14-1171-82) in Western Blot and Flow Cytometry

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

0.5 µg/test

View 96 publications 96 publications

Immunoprecipitation (IP)

Assay-Dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Pig, Mouse

Published species

Fish, Mouse

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

2B8

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_467433

Product Specific Information

Description: The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor, and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145-kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1 low) and negative for lineage markers. The interaction of the mouse c-Kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells.

Applications Reported: The 2B8 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested: This 2B8 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

c-Kit, also known as mast/stem cell growth factor receptor (SCFR) or CD117, is a trans membrane type III tyrosine kinase receptor encoded by the c-Kit proto-oncogene, located on chromosome 4 in humans. It is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: belly-spot; c-kit; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit

Gene Aliases: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; MGF; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W

UniProt ID: (Pig) Q2HWD6, (Mouse) P05532

Entrez Gene ID: (Pig) 396810, (Mouse) 16590

Molecular Function: transmembrane signal receptor