|Tested species reactivity||Mouse|
|Host / Isotype||Armenian hamster / IgG|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The monoclonal antibody 2E2 recognizes mouse CD119 also known as IFN gamma receptor1. CD119 heterodimerizes with IFN gamma receptor 2 (AF-1) forming the Class II cytokine receptor. The ligand IFN gamma binds IFN gamma receptor 1 subunit but signal transduction transpires via the IFN gamma receptor 2 subunit. The receptor is involved in host defense and expression is broad including B cells, NK cells, macrophages and dendritic cells as well as other non-hematopoietic cell types.
Applications Reported: This 2E2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 2E2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In addition, longer incubations appear to help stain the popµLation; therefore we recommend a 60 minute incubation at 2-8°C.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.