|Flow Cytometry (Flow)||1:25-1:50|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Porcine alveolar macrophages|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul. A suggested positive control for immunohistochemical applications is porcine spleen. In Western blot, this antibody detects a band at ~155 kDa in alveolar macrophage lysates under reducing conditions.
CD11R3 is a 155 kDa cell surface glycoprotein, a member of the alpha integrin family. CD11R3 has a similar expression pattern to the human CD11b marker, being expressed on granulocytes, monocytes and alveolar macrophages, but not on lymphocytes, eythrocytes or platelets.
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