|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human CD11b (Mac-1)|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X, and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, and blood monocytes. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue.
Analyte Specific Reagent
New IDH1 mutant inhibitors for treatment of acute myeloid leukemia.
CD11b00 was used in flow cytometry to assess the effects of allosteric inhibitors on different mutant forms of isocitrate dehydrogenase 1 in leukemia
|Okoye-Okafor UC,Bartholdy B,Cartier J,Gao EN,Pietrak B,Rendina AR,Rominger C,Quinn C,Smallwood A,Wiggall KJ,Reif AJ,Schmidt SJ,Qi H,Zhao H,Joberty G,Faelth-Savitski M,Bantscheff M,Drewes G,Duraiswami C,Brady P,Groy A,Narayanagari SR,Antony-Debre I,Mitchell K,Wang HR,Kao YR,Christopeit M,Carvajal L,Barreyro L,Paietta E,Makishima H,Will B,Concha N,Adams ND,Schwartz B,McCabe MT,Maciejewski J,Verma A,Steidl U||Nature chemical biology (11:878)||2015|
Modulation of neutrophil function by the tripeptide feG.
CD11b00 was used in flow cytometry to determine the effects of feG on human and rat neutrophil function.
|Mathison RD,Befus AD,Davison JS,Woodman RC||BMC immunology (4:null)||2003|
Urokinase plasminogen activator receptor, beta 2-integrins, and Src-kinases within a single receptor complex of human monocytes.
CD11b00 was used in flow cytometry to elucidate uPA-R-related cellular events.
|Bohuslav J,Horejsí V,Hansmann C,Stöckl J,Weidle UH,Majdic O,Bartke I,Knapp W,Stockinger H||The Journal of experimental medicine (181:1381)||1995|
The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands.
CD11b00 was used in flow cytometry and immunoprecipitation to map the binding sites for ligands of Mac-1.
|Diamond MS,Garcia-Aguilar J,Bickford JK,Corbi AL,Springer TA||The Journal of cell biology (120:1031)||1993|