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Invitrogen
-ELISA-20170216130641.jpg "CD120b (TNF Receptor II) Antibody (14-1203-81) in ELISA")
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
Assay-Dependent | - |
Flow Cytometry (Flow) |
Assay-Dependent | View 2 publications 2 publications |
ELISA (ELISA) |
1-4 µg/mL | - |
Immunoprecipitation (IP) |
Assay-Dependent | - |
Neutralization (Neu) |
- | View 1 publication 1 publication |
Functional Assay (FN) |
Assay-Dependent | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Mouse |
Published species |
Mouse |
Host/Isotype |
Armenian hamster / IgG |
Class |
Monoclonal |
Type |
Antibody |
Clone |
TR75-54 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C |
RRID |
AB_1257205 |
Description: The TR75-54 monoclonal antibody reacts with mouse Tumor Necrosis Factor Receptor II (TNFRII, TNFR-p80, TNFRSF1B, CD120b). TNFRII is expressed in a variety of cell types and strongly expressed on stimulated T and B lymphocytes. TNFRII is the main TNF receptor found on circulating T cells and is the major mediator of autoregulatory apoptosis in CD8+ cells. The soluble TNF receptors are truncated forms of cell surface receptors with neutralizing activity on both TNF-alpha and TNF-beta. It has been suggested that the proinflammatory and immunosuppressive properties of TNF segregate at the level of its receptors and that the pool of TNF bound to soluble receptors could represent a reservoir to control the TNF release.
Applications Reported: This TR75-54 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and ELISA.
Applications Tested: The TR75-54 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse TNFRII in combination with the biotin TR75-32 (cat. 13-1204) antibody for detection and recombinant mouse TNFRII as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD120b; p75; p75 TNFR; p80 TNF-alpha receptor; sCD120b; soluble CD120b; soluble TNFR2; sTNF R2; sTNFR 2; sTNFR2; TNF receptor beta chain; TNF-R2; TNF-RII; TNFR; TNFR II; TNFR-II; Tumor necrosis factor receptor; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor type II
Gene Aliases: CD120b; p75; TNF-alphaR2; TNF-R-II; TNF-R2; TNF-R75; TNFalpha-R2; TNFBR; Tnfr-1; Tnfr-2; Tnfr2; TNFR80; TNFRII; Tnfrsf1b
UniProt ID: (Mouse) P25119
Entrez Gene ID: (Mouse) 21938
Molecular Function:
transmembrane signal receptor
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