Invitrogen

CD120b (TNF Receptor II) Monoclonal Antibody (TR75-54), eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (1)

CD120b (TNF Receptor II) Antibody in ELISA (ELISA)

FIGURE: 1 / 2

CD120b (TNF Receptor II) Antibody (14-1203-81) in ELISA

Standard curve of Mouse CD120b (TNF Receptor II) ELISA.

Product Image Gallery

CD120b (TNF Receptor II) Antibody in Neutralization (Neu) — 7 FIGURE Recombinant IFN-beta treatment reduces TNF-alpha/TNFR1-induced apoptosis in Ifnb -/- primary neurons. (A) Normalized Tnfa qPCR expression in Wt vs Ifnb -/- primary CGNs, n = 3 per genotype. (B) Wt vs Ifnb -/- CGN viability (count), n = 5 per genotype. (C) rTNF-alpha (10 ng/mL)-treated Wt CGNs, normalized to untreated (UT), n = 5 per genotype. (D) Ifnb -/- CGN counts, treated with neutralizing anti (alpha)-TNF-alpha antibody (10 mug/mL) or immunoglobulin (IgG) control, n = 8 per condition. (E) Normalized Tnfr1 qPCR expression in untreated versus rIFN-beta-treated (24 h, 100 U/mL) Ifnb -/- CGNs, n = 3 representing one of three biological replicates. (A-E) * p < 0.05, *** p < 0.001, and **** p < 0.0001 by t- test. (F-G) Normalized Tnfr1 and Casp3 qPCR expression in CGNs, n = 3 representing one of three independent experiments per genotype. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 by one-way ANOVA and Tukey's post hoc test. (H) IF-labeled Casp3 + (green) DAPI + (blue) neurons as %DAPI+ CGNs. Scale bar, 25 mum; n = 4-7 per genotype. * p < 0.05 and ** p < 0.01 by one-way ANOVA and Tukey's post hoc test. (I) Truncated (t)-BID/BID levels in CGNs, untreated or treated 24 h with rTNF-alpha (10 ng/mL), n = 3-7 per condition. * p < 0.05 by two-way ANOVA and Dunnett's post hoc test. # p < 0.05 by t -test. (J-K) IF-labeled Casp3 + DAPI + neurons as %DAPI + CGNs, untreated or treated 24 h with rTNF-alpha (10 ng/mL) or rIFN-beta (100 U/mL). Scale ba

Product Details

14-1203-81

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Flow Cytometry (Flow)

Assay-Dependent

View 2 publications 2 publications

ELISA (ELISA)

1-4 µg/mL

Immunoprecipitation (IP)

Assay-Dependent

Neutralization (Neu)

View 2 publications 2 publications

Functional Assay (FN)

Assay-Dependent

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

TR75-54

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_1257205

Product Specific Information

Description: The TR75-54 monoclonal antibody reacts with mouse Tumor Necrosis Factor Receptor II (TNFRII, TNFR-p80, TNFRSF1B, CD120b). TNFRII is expressed in a variety of cell types and strongly expressed on stimulated T and B lymphocytes. TNFRII is the main TNF receptor found on circulating T cells and is the major mediator of autoregulatory apoptosis in CD8+ cells. The soluble TNF receptors are truncated forms of cell surface receptors with neutralizing activity on both TNF-alpha and TNF-beta. It has been suggested that the proinflammatory and immunosuppressive properties of TNF segregate at the level of its receptors and that the pool of TNF bound to soluble receptors could represent a reservoir to control the TNF release.

Applications Reported: This TR75-54 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and ELISA.

Applications Tested: The TR75-54 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse TNFRII in combination with the biotin TR75-32 (cat. 13-1204) antibody for detection and recombinant mouse TNFRII as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neuronal TNFα, Not α-Syn, Underlies PDD-Like Disease Progression in IFNβ-KO Mice.

Annals of neurology

Villanueva EB,Tresse E,Liu Y,Duarte JN,Jimenez-Duran G,Ejlerskov P,Kretz O,Loreth D,Goldmann T,Prinz M,Issazadeh-Navikas S

Published figure using CD120b (TNF Receptor II) monoclonal antibody (Product # 14-1203-81) in Neutralization

Mon Nov 01 00:00:00 EDT 2021

Preferential TNFα signaling via TNFR2 regulates epithelial injury and duct obstruction in experimental biliary atresia.

JCI insight

Shivakumar P,Mizuochi T,Mourya R,Gutta S,Yang L,Luo Z,Bezerra JA

14-1203-81 was used in Neutralization to determine factors regulating the pathogenic mechanisms of biliary atresia.

Thu Mar 09 00:00:00 EST 2017

Effector lymphocyte-induced lymph node-like vasculature enables naive T-cell entry into tumours and enhanced anti-tumour immunity.

Nature communications

Peske JD,Thompson ED,Gemta L,Baylis RA,Fu YX,Engelhard VH

14120381 was used in flow cytometry to find that lymph node-like vasculature in melanoma and lung carcinoma murine models is both a consequence of and key contributor to anti-tumor immunity

Wed May 13 00:00:00 EDT 2015

Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells.

Nature immunology

Mahmud SA,Manlove LS,Schmitz HM,Xing Y,Wang Y,Owen DL,Schenkel JM,Boomer JS,Green JM,Yagita H,Chi H,Hogquist KA,Farrar MA

14-1203 was used in Flow cytometry/Cell sorting to study the role of TNF receptors in the thymic development of Treg cells..

Thu May 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD120b; p75; p75 TNFR; p80 TNF-alpha receptor; sCD120b; soluble CD120b; soluble TNFR2; sTNF R2; sTNFR 2; sTNFR2; TNF receptor beta chain; TNF-R2; TNF-RII; TNFR; TNFR II; TNFR-II; Tumor necrosis factor receptor; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor type II

View more View less

Gene Aliases: CD120b; p75; TNF-alphaR2; TNF-R-II; TNF-R2; TNF-R75; TNFalpha-R2; TNFBR; Tnfr-1; Tnfr-2; Tnfr2; TNFR80; TNFRII; Tnfrsf1b

View more View less

UniProt ID: (Mouse) P97893

View more View less

Entrez Gene ID: (Mouse) 21938

View more View less

Molecular Function: transmembrane signal receptor

Function(s)

inflammatory response immune response cell surface receptor signaling pathway cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage programmed cell death tumor necrosis factor-mediated signaling pathway negative regulation of inflammatory response RNA destabilization positive regulation of membrane protein ectodomain proteolysis cellular response to lipopolysaccharide extrinsic apoptotic signaling pathway

Process(es)

tumor necrosis factor-activated receptor activity protein binding ubiquitin protein ligase binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD120b (TNF Receptor II) Monoclonal Antibody (TR75-54), eBioscience™

CD120b (TNF Receptor II) Antibody (14-1203-81) in ELISA

Product Details

14-1203-81

Western Blot (WB)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!