|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Mouse myeloma cell line NS0-derived recombinant human IL-7 R alpha Extracellular domain|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2.5 µg/10^6 cells|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blots, approximately 5% cross-reactivity was observed with recombinant human (rh) IL-10 R, rhIL-2 R beta, rhIL-5 R alpha, and rhIL-6 R.
Reconstitute at 0.5 mg/mL in sterile PBS.
Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre B, pro B, and early T cells. It was previously known as pre B cell growth factor and lymphopoietin 1. IL7 is a glycoprotein which promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. The functional high-affinity IL7 receptor consists of an alpha chain and a gamma chain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.