|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1/IgG2b|
|Immunogen||human cell line KG-1a|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 0.2% carrier protein|
|Contains||0.08% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is a monoclonal mixture of FITC-conjugated CD14 (clone FWKW, isotype IgG2b) and PE-conjugated CD13 (clone E735, isotype IgG1).
CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS)-dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram-negative shock. This antibody recognizes the human CD13 cell surface glycoprotein, a 150kD molecule expressed by granulocytes and monocytes, and by myeloid leukemia cells. Inhibits infection of cells by human coronavirus, inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates. MA1-22542 detects CD13 in Human samples.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.