Invitrogen

CD130 (GP130) Monoclonal Antibody (KGP130), APC, eBioscience™

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Antibody Testing Data (1)
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CD130 (GP130) Antibody in Flow Cytometry (Flow)

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CD130 (GP130) Antibody (17-1302-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.125 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.125 µg of Anti-Mouse CD130 APC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-1302-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

KGP130

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10670874

Product Specific Information

Description: The KGP130 monoclonal antibody reacts with mouse gp130, also known as CD130. gp130 is a 130 kD Type-I transmembrane glycoprotein containing a 597 amino acid extracellular domain, a single transmembrane domain, and a 277 amino acid cytoplasmic domain. gp130 is a subunit of several heterodimeric cell-surface receptors, including receptors for IL-6, IL-11, IL-27, Oncostatin M, and Leukemia Inhibitory Factor (LIF). The gp130 protein has also been found to exist in a soluble form, which is capable of inhibiting IL-6 signaling. gp130 is expressed mainly on T cells, monocytes, endothelial cells, activated B cells, and plasma cells, and is expressed at lower levels on most leukocytes and epithelial cells. In response to ligand binding, gp130 becomes tyrosine phosphorylated, leading to activation of several signaling pathways including the PI3 kinase, Ras-MAPk and Stat pathways.

Preincubation of mouse splenic T cells with IL-6 is capable of blocking the interaction of KGP130 with gp130, suggesting that the KGP130 binding site lies near the IL-6 interaction domain. Reactivity of KGP130 towards human gp130 has not been observed.

Applications Reported: This KGP130 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This KGP130 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The persistence of interleukin-6 is regulated by a blood buffer system derived from dendritic cells.

Immunity

Yousif AS,Ronsard L,Shah P,Omatsu T,Sangesland M,Bracamonte Moreno T,Lam EC,Vrbanac VD,Balazs AB,Reinecker HC,Lingwood D

17-1302 was used in Flow cytometry/Cell sorting to find conventional dendritic cells (cDCs), defined in mice via expression of the transcription factor Zbtb46, were a major source of circulating sIL-6R and, thus, systemically regulated IL-6 signaling.

Tue Feb 09 00:00:00 EST 2021

The persistence of interleukin-6 is regulated by a blood buffer system derived from dendritic cells.

Immunity

Yousif AS,Ronsard L,Shah P,Omatsu T,Sangesland M,Bracamonte Moreno T,Lam EC,Vrbanac VD,Balazs AB,Reinecker HC,Lingwood D

Tue Feb 09 00:00:00 EST 2021

Interleukin (IL)-39 [IL-23p19/Epstein-Barr virus-induced 3 (Ebi3)] induces differentiation/expansion of neutrophils in lupus-prone mice.

Clinical and experimental immunology

Wang X,Liu X,Zhang Y,Wang Z,Zhu G,Han G,Chen G,Hou C,Wang T,Ma N,Shen B,Li Y,Xiao H,Wang R

17-1302 was used in Flow cytometry/Cell sorting to suggest that IL-39 induces differentiation and/or expansion of neutrophils in lupus-prone mice.

Tue Nov 01 00:00:00 EDT 2016

The adaptor TRAF5 limits the differentiation of inflammatory CD4(+) T cells by antagonizing signaling via the receptor for IL-6.

Nature immunology

Nagashima H,Okuyama Y,Asao A,Kawabe T,Yamaki S,Nakano H,Croft M,Ishii N,So T

17-1302 was used in Conjugation experiments to investigate how the adaptor TRAF5 limits the differentiation of inflammatory CD4(+) T cells.

Thu May 01 00:00:00 EDT 2014

Kupffer cells protect liver sinusoidal endothelial cells from Fas-dependent apoptosis in sepsis by down-regulating gp130.

The American journal of pathology

Hutchins NA,Chung CS,Borgerding JN,Ayala CA,Ayala A

17-1302 was used in Flow cytometry/Cell sorting to document a role for death receptor-mediated liver sinusoidal endothelial cell (LSEC) injury and show that Kupffer cells are essential to the viability of LSECs.

Fri Mar 01 00:00:00 EST 2013

gp130, the cytokine common signal-transducer of interleukin-6 cytokine family, is downregulated in T cells in vivo by interleukin-6.

Blood

Wang XJ,Taga T,Yoshida K,Saito M,Kishimoto T,Kikutani H

Fri May 01 00:00:00 EDT 1998

Bioinformatics

Protein Aliases: CD130; gp130; GP130-RAPS; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; IL6R-beta; Interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; Oncostatin-M receptor subunit alpha

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Gene Aliases: 5133400A03Rik; AA389424; BB405851; CD130; D13Ertd699e; gp130; Il6st

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UniProt ID: (Mouse) Q00560

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Entrez Gene ID: (Mouse) 16195

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Function(s)

cytokine receptor activity ciliary neurotrophic factor receptor activity interleukin-6 receptor activity interleukin-11 receptor activity leukemia inhibitory factor receptor activity oncostatin-M receptor activity ciliary neurotrophic factor receptor binding interleukin-6 receptor binding protein binding growth factor binding interleukin-11 binding interleukin-6 binding protein heterodimerization activity transmembrane signal receptor

Process(es)

glycogen metabolic process triglyceride mobilization signal transduction positive regulation of cell proliferation regulation of Notch signaling pathway positive regulation of smooth muscle cell migration cytokine-mediated signaling pathway response to cytokine oncostatin-M-mediated signaling pathway positive regulation of T cell proliferation positive regulation of tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of osteoblast differentiation positive regulation of astrocyte differentiation leukemia inhibitory factor signaling pathway negative regulation of cytosolic calcium ion concentration interleukin-6-mediated signaling pathway negative regulation of interleukin-6-mediated signaling pathway interleukin-27-mediated signaling pathway ciliary neurotrophic factor-mediated signaling pathway

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD130 (GP130) Monoclonal Antibody (KGP130), APC, eBioscience™

CD130 (GP130) Antibody (17-1302-82) in Flow

Product Details

17-1302-82

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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