|Tested species reactivity||Rat|
|Published species reactivity||Cat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Phytohaemagglutinin-activated rat lymph node cells.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/1 million cells|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 2 publications below|
MA1-70020 detects CD134 in rat samples.
MA1-70020 has been successfully used in flow cytometry, immunoprecipitation and immunohistochemistry (frozen sections) procedures.
The MA1-70020 immunogen is Phytohaemagglutinin-activated rat lymph node cells.
CD134, also known as OX40, is a member of the TNFR-superfamily of receptors which is not constitutively expressed on resting naïve T cells, unlike CD28. OX40 is a secondary costimulatory molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation. Expression of OX40 is dependent on full activation of the T cell; without CD28, expression of OX40 is delayed and of fourfold lower levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Induction of feline immunodeficiency virus from a chronically infected feline T-lymphocyte cell line.
MA1-70020 was used in flow cytometry to evaluate a chronically infected feline T-lymphocyte cell line able to inducibly express feline immunodeficiency virus
|Tochikura TS,Naito Y,Kozutsumi Y,Hohdatsu T||Research in veterinary science (92:327)||2012|
Differential CXCR4 expression and function in subpopulations of the feline lymphoma cell line 3201 susceptible to feline immunodeficiency virus.
MA1-70020 was used in flow cytometry to detect FIV replication in feline cell culture
|Tochikura TS,Motokawa K,Naito Y,Kozutsumi Y,Tanabe-Tochikura A,Hohdatsu T||Journal of feline medicine and surgery (12:269)||2010|