Description: The monoclonal antibody OX40 reacts with rat CD134, a 50-kDa glycoprotein belonging to the TNFR superfamily. CD134, which is also known as OX40, is expressed on activated T cells, TH1, TH2, TH17, and Tregs, but not on naive CD4 or CD8 T cells or most memory cells. CD134 is also inducible on NKT cells, neutrophils, and NK cells. CD134 binds to CD252 (OX40L) to mediate T cell expansion and survival, as well as memory T cell generation. Finally, CD134 is involved in Treg suppression and tolerance.
Applications Reported: This OX40 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This OX40 antibody has been tested by flow cytometric analysis of stimulated rat splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Tumor necrosis factor receptor superfamily, member4, also known as ACT35 or CD134 is a cell surface glycoprotein that was discovered through the production of a monoclonal antibody raised against the HUT-102 cell line. It belongs to the tumor necrosis factor receptor superfamily. CD134 was mapped to 1p36 by fluorescence in situ hybridization. CD134 is the primary receptor for feline immunodeficiency virus. CD134 expression can promote viral binding and renders cells permissive for viral entry, productive infection, and syncytium formation. Stimulating the receptor can improve the response to a powerful virus vector and may be useful in vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD134; MRC OX40; OX40 antigen; OX40L receptor; Tax-transcriptionally activated glycoprotein 1; tumor necrosis factor (ligand) superfamily member 4; Tumor necrosis factor receptor superfamily member 4; tumor necrosis factor superfamily, member 4
Gene Aliases: Ox40; Tnfrsf4; Txgp1; Txgp1l
UniProt ID: (Rat) P15725
Entrez Gene ID: (Rat) 25572