Invitrogen

CD137 Ligand (4-1BB Ligand) Monoclonal Antibody (TKS-1), Biotin, eBioscience™

Product Image Gallery

CD137 Ligand (4-1BB Ligand) Antibody in Flow Cytometry (Flow) — Staining of mouse splenocytes unstimulated (left) or Anti-Mouse IgM plus Anti-Mouse CD40 Functional Grade Purified (Product # 16-0402-82 stimulated splenocytes (right) with Anti-Mouse 4-1BB Ligand PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Product Details

13-5901-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	1 µg/test	View 3 publications 3 publications

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	TKS-1
Conjugate	Biotin PE PerCP-eFluor 710
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_466788

Product Specific Information

Description: The TKS-1 monoclonal antibody reacts with mouse 4-1BB Ligand (4-1BBL), a type II transmembrane protein and a member of the tumor necrosis factor superfamily. 4-1BBL is expressed by activated antigen presenting cells including B cells and macrophages. Interaction of 4-1BBL with 4-1BB (CDw137) is reported to deliver a costimulatory signal important in the T-APC interaction.

Applications Reported: The TKS-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The TKS-1 antibody has been tested by flow cytometric analysis of resting and 2-3 day (IgM & CD40)-activated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. CD137L is a co-stimulatory molecule that is expressed on antigen presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation. Its receptor, CD137/4-1BB, is found on a variety of cells, including inflamed endothelial cells, where its expression enhances extravasation of CD137L expressing monocytes. Activated T cells also express CD137, and engagement with CD137L enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity. CD137L is also expressed on a majority of B-cell lymphomas, including mantle cell lymphoma, follicular lymphoma, and diffuse large B-cell lymphoma (but it is lacking on Hodgkin lymphoma and T-cell lymphoma). CD137L is also expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

4-1BB ligand signaling to T cells limits T cell activation.

Journal of immunology (Baltimore, Md. : 1950)

Eun SY,Lee SW,Xu Y,Croft M

13-5901 was used in Flow cytometry/Cell sorting to show that 4-1BBL can transmit signals that limit T cell effector activity under tolerogenic conditions.

Thu Jan 01 00:00:00 EST 2015

Impact of enzymatic tissue disintegration on the level of surface molecule expression and immune cell function.

European journal of microbiology & immunology

Autengruber A,Gereke M,Hansen G,Hennig C,Bruder D

13-5901 was used in Flow cytometry/Cell sorting to indicate that enzymatic tissue disintegration can have profound effects on the expression of a variety of cell-surface molecules.

Fri Jun 01 00:00:00 EDT 2012

The 4-1BB costimulation augments the proliferation of CD4+CD25+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Zheng G,Wang B,Chen A

Sun Aug 15 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: 4 1 BB Ligand; 4 1 BBL; 4-1BB ligand; 4-1BBL; 41BB Ligand; 41BBL; CD 137 Ligand; CD 137L; CD137 Ligand; CDw137L; TNFRSF9; Tumor necrosis factor ligand superfamily member 9

Gene Aliases: 4-1BB-L; 4-1BBL; AI848817; Cd137l; Cd157l; Ly63l; Tnfsf9

UniProt ID: (Mouse) P41274

Entrez Gene ID: (Mouse) 21950

Function(s)

immune response cell-cell signaling positive regulation of interferon-gamma production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of activated T cell proliferation regulation of cell proliferation myeloid dendritic cell differentiation positive regulation of cytotoxic T cell differentiation

Process(es)

receptor binding cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding

CD137 Ligand (4-1BB Ligand) Monoclonal Antibody (TKS-1), Biotin, eBioscience™

Product Details

13-5901-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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