Flow cytometry analysis of CD137 in PBMC cells stimulated with PHA for 3 days (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # MA5-13739) at a dilution of 1 ug/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Ectodomain of human 4-1BB recombinant protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13736 targets CD137 in FACS and IF applications and shows reactivity with Human samples.
The MA5-13736 immunogen is ectodomain of human 4-1BB recombinant protein.
4-1BB (also known as CD137) is an inducible receptor-like protein expressed on the cell surface of activated splenic T cells and thymocytes. It exists as both a monomer and a dimer on the surface of activated T cells. 4-1BB is structurally related to the members of NGFR/TNFR superfamily which are characterized by the presence of three-six patterns of a cysteine-rich motif in their extracellular domains. Other members of this family include low affinity NGFR, two receptors for TNF (TNFR-I and TNFR-II), CD30, CD40, OX40, Fas, and CD27. These molecules are involved in cell growth, survival, and death processes. The cytoplasmic domain of 4-1BB include two runs of acidic amino acids, a potential p56lck binding site, five consecutive glycines at the C-terminus, and four potential phosphorylation sites: one tyrosine, two threonine, and one serine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Expression of CD137 on Hodgkin and Reed-Sternberg cells inhibits T-cell activation by eliminating CD137 ligand expression.
MA5-13736 was used in immunohistochemistry to study the role of CD137 on Hodgkin and Reed-Sternberg cells in the mechanism by which they inhibit the activation of T-cells
|Ho WT,Pang WL,Chong SM,Castella A,Al-Salam S,Tan TE,Moh MC,Koh LK,Gan SU,Cheng CK,Schwarz H||Cancer research (73:652)||2013|
CD137 is expressed in follicular dendritic cell tumors and in classical Hodgkin and T-cell lymphomas: diagnostic and therapeutic implications.
MA5-13736 was used in immunohistochemistry to study the diagnostic and therapeutic importance of CD137 expression by follicular dendritic cell tumors and Hodgkin and T-cell lymphomas
|Anderson MW,Zhao S,Freud AG,Czerwinski DK,Kohrt H,Alizadeh AA,Houot R,Azambuja D,Biasoli I,Morais JC,Spector N,Molina-Kirsch HF,Warnke RA,Levy R,Natkunam Y||The American journal of pathology (181:795)||2012|
Prediction of survival in diffuse large B-cell lymphoma based on the expression of 2 genes reflecting tumor and microenvironment.
MA5-13736 was used in immunohistochemistry to study the expression of genes reflecting tumor and microenvironment and their value in predicting survival in diffuse large B-cell lymphoma
|Alizadeh AA,Gentles AJ,Alencar AJ,Liu CL,Kohrt HE,Houot R,Goldstein MJ,Zhao S,Natkunam Y,Advani RH,Gascoyne RD,Briones J,Tibshirani RJ,Myklebust JH,Plevritis SK,Lossos IS,Levy R||Blood (118:1350)||2011|
Induction of proliferation and monocytic differentiation of human CD34+ cells by CD137 ligand signaling.
MA5-13736 was used in immunohistochemistry to investigate the influence of CD137 on CD34+ cell amplification and differentiation
|Jiang D,Yue PS,Drenkard D,Schwarz H||Stem cells (Dayton, Ohio) (26:2372)||2008|
CD137 enhances monocyte-ICAM-1 interactions in an E-selectin-dependent manner under flow conditions.
MA5-13736 was used in flow cytometry to study the role of CD137 in monocyte recruitment to inflammation
|Quek BZ,Lim YC,Lin JH,Tan TE,Chan J,Biswas A,Schwarz H||Molecular immunology (47:1839)||2010|
CD137 ligand mediates opposite effects in human and mouse NK cells and impairs NK-cell reactivity against human acute myeloid leukemia cells.
MA5-13736 was used in flow cytometry to study the role of CD137 ligand in NK-cell reactivity against human acute myeloid leukemia cells
|Baessler T,Charton JE,Schmiedel BJ,Grünebach F,Krusch M,Wacker A,Rammensee HG,Salih HR||Blood (115:3058)||2010|
Molecular mechanisms and gene regulation of melphalan- and hyperthermia-induced apoptosis in Ewing sarcoma cells.
MA5-13736 was used in blocking or activating experiment to study the mechanisms underlying melphalan- and hyperthermia-induced apoptosis in Ewing sarcoma cells
|Krause C,Klüttermann K,Mauz-Körholz C||Anticancer research (28:2585)||2008|
Constitutive expression of functional 4-1BB (CD137) ligand on carcinoma cells.
MA5-13736 was used in western blot to study the constitutive expression of functional 4-1BB (CD137) ligand on carcinoma cells
|Salih HR,Kosowski SG,Haluska VF,Starling GC,Loo DT,Lee F,Aruffo AA,Trail PA,Kiener PA||Journal of immunology (Baltimore, Md. : 1950) (165:2903)||2000|