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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Tthe CD14 antigen|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 2 publications below|
CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Differential leukocyte counting and immunophenotyping in cryopreserved ex vivo whole blood.
Q10013 was used in flow cytometry to compared leukocyte proportions and absolute counts using reference real-time methods with a novel assay that allows long-term cryopreservation of fixed leukocytes for later counting
|Nemes E,Kagina BM,Smit E,Africa H,Steyn M,Hanekom WA,Scriba TJ||Cytometry. Part A : the journal of the International Society for Analytical Cytology (87:157)||2015|
Variations in the heme oxygenase-1 microsatellite polymorphism are associated with plasma CD14 and viral load in HIV-infected African-Americans.
Q10013 was used in flow cytometry to study heme oxygenase- in HIV-infected people.
|Seu L,Burt TD,Witte JS,Martin JN,Deeks SG,McCune JM||Genes and immunity (13:258)||2012|
monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein