Invitrogen

CD14 Monoclonal Antibody (61D3), APC-eFluor™ 780, eBioscience™

Product Image Gallery

CD14 Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (blue histogram) or Anti-Human CD14 APC-eFluor® 780 (purple histogram). Cells in the monocyte gate were used for analysis.

Product Details

47-0149-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.5 µg)/test	View 26 publications 26 publications

Product Specifications
Species Reactivity	Human
Published species	Human, Non-human primate, Rhesus monkey
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	61D3
Conjugate	APC-eFluor™ 780 Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 660 Alexa Fluor 700 APC Biotin Brilliant UV 395 Brilliant UV 496 Brilliant UV 615 Brilliant UV 661 Brilliant UV 737 Brilliant UV 805 Brilliant Violet 421 Brilliant Violet 480 Brilliant Violet 650 Brilliant Violet 711 Brilliant Violet 786 eFluor 450 eFluor 506 FITC Functional Grade NovaFluor Blue 510 NovaFluor Blue 555 NovaFluor Blue 585
Excitation/Emission Max	756/785 nm View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_1834358

Product Specific Information

Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.

Applications Reported: This 61D3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Changes in expression of PD-L1 on peripheral T cells in patients with melanoma and lung cancer treated with PD-1 inhibitors.

Scientific reports

Dart SJ,Cook AM,Millward MJ,McDonnell AM,Chin WL,Hakeem MU,Meniawy TM,Bowyer SE

47-0149-42 was used in Flow Cytometry to identify patients before or early in treatment who would best respond to PD-1 inhibitors.

Wed Jul 28 00:00:00 EDT 2021

Naturally enhanced neutralizing breadth against SARS-CoV-2 one year after infection.

Nature

Wang Z,Muecksch F,Schaefer-Babajew D,Finkin S,Viant C,Gaebler C,Hoffmann HH,Barnes CO,Cipolla M,Ramos V,Oliveira TY,Cho A,Schmidt F,Da Silva J,Bednarski E,Aguado L,Yee J,Daga M,Turroja M,Millard KG,Jankovic M,Gazumyan A,Zhao Z,Rice CM,Bieniasz PD,Caskey M,Hatziioannou T,Nussenzweig MC

47-0149-42 was used in Flow Cytometry to report on a cohort of 63 individuals who have recovered from COVID-19 assessed at 1.3, 6.2 and 12 months after SARS-CoV-2 infection.

Thu Jul 01 00:00:00 EDT 2021

Naturally enhanced neutralizing breadth to SARS-CoV-2 after one year.

bioRxiv : the preprint server for biology

Wang Z,Muecksch F,Schaefer-Babajew D,Finkin S,Viant C,Gaebler C,Hoffmann HH,Barnes CO,Cipolla M,Ramos V,Oliveira TY,Cho A,Schmidt F,da Silva J,Bednarski E,Aguado L,Yee J,Daga M,Turroja M,Millard KG,Jankovic M,Gazumyan A,Zhao Z,Rice CM,Bieniasz PD,Caskey M,Hatziioannou T,Nussenzweig MC

47-0149-42 was used in Flow Cytometry to report on a cohort of 63 individuals who have recovered from COVID-19 assessed at 1.3, 6.2 and 12 months after SARS-CoV-2 infection, 41% of whom also received mRNA vaccines.

Wed Jun 02 00:00:00 EDT 2021

Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease.

The Journal of experimental medicine

Agudelo M,Palus M,Keeffe JR,Bianchini F,Svoboda P,Salát J,Peace A,Gazumyan A,Cipolla M,Kapoor T,Guidetti F,Yao KH,Elsterová J,Teislerová D,Chrdle A,Hönig V,Oliveira T,West AP,Lee YE,Rice CM,MacDonald MR,Bjorkman PJ,Růžek D,Robbiani DF,Nussenzweig MC

47-0149-42 was used in Flow Cytometry to report on the human neutralizing antibody response to TBEV in a cohort of infected and vaccinated individuals.

Mon May 03 00:00:00 EDT 2021

mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants.

Nature

Wang Z,Schmidt F,Weisblum Y,Muecksch F,Barnes CO,Finkin S,Schaefer-Babajew D,Cipolla M,Gaebler C,Lieberman JA,Oliveira TY,Yang Z,Abernathy ME,Huey-Tubman KE,Hurley A,Turroja M,West KA,Gordon K,Millard KG,Ramos V,Da Silva J,Xu J,Colbert RA,Patel R,Dizon J,Unson-O'Brien C,Shimeliovich I,Gazumyan A,Caskey M,Bjorkman PJ,Casellas R,Hatziioannou T,Bieniasz PD,Nussenzweig MC

47-0149-42 was used in Flow Cytometry to report on the antibody and memory B cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer-BioNTech (BNT162b2) vaccine against SARS-CoV-21-4.

Thu Apr 01 00:00:00 EDT 2021

Evolution of antibody immunity to SARS-CoV-2.

Nature

Gaebler C,Wang Z,Lorenzi JCC,Muecksch F,Finkin S,Tokuyama M,Cho A,Jankovic M,Schaefer-Babajew D,Oliveira TY,Cipolla M,Viant C,Barnes CO,Bram Y,Breton G,Hägglöf T,Mendoza P,Hurley A,Turroja M,Gordon K,Millard KG,Ramos V,Schmidt F,Weisblum Y,Jha D,Tankelevich M,Martinez-Delgado G,Yee J,Patel R,Dizon J,Unson-O'Brien C,Shimeliovich I,Robbiani DF,Zhao Z,Gazumyan A,Schwartz RE,Hatziioannou T,Bjorkman PJ,Mehandru S,Bieniasz PD,Caskey M,Nussenzweig MC

47-0149 was used in Flow cytometry/Cell sorting to report on the humoral memory response in a cohort of 87 individuals assessed at 1.3 and 6.2 months after infection with SARS-CoV-2.

Mon Mar 01 00:00:00 EST 2021

mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants.

bioRxiv : the preprint server for biology

47-0149 was used in Flow cytometry/Cell sorting to suggest that the monoclonal antibodies in clinical use should be tested against newly arising variants, and that mRNA vaccines may need to be updated periodically to avoid potential loss of clinical efficacy.

Sat Jan 30 00:00:00 EST 2021

Evolution of Antibody Immunity to SARS-CoV-2.

bioRxiv : the preprint server for biology

Gaebler C,Wang Z,Lorenzi JCC,Muecksch F,Finkin S,Tokuyama M,Cho A,Jankovic M,Schaefer-Babajew D,Oliveira TY,Cipolla M,Viant C,Barnes CO,Hurley A,Turroja M,Gordon K,Millard KG,Ramos V,Schmidt F,Weisblum Y,Jha D,Tankelevich M,Yee J,Shimeliovich I,Robbiani DF,Zhao Z,Gazumyan A,Hatziioannou T,Bjorkman PJ,Mehandru S,Bieniasz PD,Caskey M,Nussenzweig MC,Hagglof T,Schwartz RE,Bram Y,Martinez-Delgado G,Mendoza P,Breton G,Dizon J,Unson-O'Brien C,Patel R

47-0149 was used in Flow cytometry/Cell sorting to conclude that the memory B cell response to SARS-CoV-2 evolves between 1.3 and 6.2 months after infection in a manner that is consistent with antigen persistence.

Mon Jan 04 00:00:00 EST 2021

Antigen-Specific Adaptive Immunity to SARS-CoV-2 in Acute COVID-19 and Associations with Age and Disease Severity.

Cell

Rydyznski Moderbacher C,Ramirez SI,Dan JM,Grifoni A,Hastie KM,Weiskopf D,Belanger S,Abbott RK,Kim C,Choi J,Kato Y,Crotty EG,Kim C,Rawlings SA,Mateus J,Tse LPV,Frazier A,Baric R,Peters B,Greenbaum J,Ollmann Saphire E,Smith DM,Sette A,Crotty S

47-0149 was used in Flow cytometry/Cell sorting to complete a combined examination of all three branches of adaptive immunity at the level of SARS-CoV-2-specific CD4+ and CD8+ T cell and neutralizing antibody responses in acute and convalescent subjects.

Thu Nov 12 00:00:00 EST 2020

A broadly neutralizing macaque monoclonal antibody against the HIV-1 V3-Glycan patch.

eLife

Wang Z,Barnes CO,Gautam R,Cetrulo Lorenzi JC,Mayer CT,Oliveira TY,Ramos V,Cipolla M,Gordon KM,Gristick HB,West AP,Nishimura Y,Raina H,Seaman MS,Gazumyan A,Martin M,Bjorkman PJ,Nussenzweig MC,Escolano A

47-0149 was used in Flow cytometry/Cell sorting to report on a monoclonal antibody, Ab1485, isolated from a macaque infected with SHIVAD8 that developed broadly neutralizing serologic activity targeting the V3-glycan region of HIV-1 Env.

Wed Oct 21 00:00:00 EDT 2020

Vascular occlusion by neutrophil extracellular traps in COVID-19.

EBioMedicine

Leppkes M,Knopf J,Naschberger E,Lindemann A,Singh J,Herrmann I,Stürzl M,Staats L,Mahajan A,Schauer C,Kremer AN,Völkl S,Amann K,Evert K,Falkeis C,Wehrfritz A,Rieker RJ,Hartmann A,Kremer AE,Neurath MF,Muñoz LE,Schett G,Herrmann M

47-0149 was used in Flow cytometry/Cell sorting to show that severe COVID-19 is characterized by a highly pronounced formation of neutrophil extracellular traps (NETs) inside the micro-vessels.

Sat Aug 01 00:00:00 EDT 2020

Convergent antibody responses to SARS-CoV-2 in convalescent individuals.

Nature

Robbiani DF,Gaebler C,Muecksch F,Lorenzi JCC,Wang Z,Cho A,Agudelo M,Barnes CO,Gazumyan A,Finkin S,Hägglöf T,Oliveira TY,Viant C,Hurley A,Hoffmann HH,Millard KG,Kost RG,Cipolla M,Gordon K,Bianchini F,Chen ST,Ramos V,Patel R,Dizon J,Shimeliovich I,Mendoza P,Hartweger H,Nogueira L,Pack M,Horowitz J,Schmidt F,Weisblum Y,Michailidis E,Ashbrook AW,Waltari E,Pak JE,Huey-Tubman KE,Koranda N,Hoffman PR,West AP,Rice CM,Hatziioannou T,Bjorkman PJ,Bieniasz PD,Caskey M,Nussenzweig MC

47-0149 was used in Flow cytometry/Cell sorting to investigate if convalescent plasma samples obtained from individuals who recover from COVID-19 contain high levels of neutralizing activity.

Sat Aug 01 00:00:00 EDT 2020

Convergent Antibody Responses to SARS-CoV-2 Infection in Convalescent Individuals.

bioRxiv : the preprint server for biology

Robbiani DF,Gaebler C,Muecksch F,Lorenzi JCC,Wang Z,Cho A,Agudelo M,Barnes CO,Gazumyan A,Finkin S,Hagglof T,Oliveira TY,Viant C,Hurley A,Hoffmann HH,Millard KG,Kost RG,Cipolla M,Gordon K,Bianchini F,Chen ST,Ramos V,Patel R,Dizon J,Shimeliovich I,Mendoza P,Hartweger H,Nogueira L,Pack M,Horowitz J,Schmidt F,Weisblum Y,Michailidis E,Ashbrook AW,Waltari E,Pak JE,Huey-Tubman KE,Koranda N,Hoffman PR,West AP,Rice CM,Hatziioannou T,Bjorkman PJ,Bieniasz PD,Caskey M,Nussenzweig MC

47-0149 was used in Flow cytometry/Cell sorting to conclude that most convalescent plasmas obtained from individuals who recover from COVID-19 do not contain high levels of neutralizing activity.

Fri May 22 00:00:00 EDT 2020

Single-Cell Analyses Inform Mechanisms of Myeloid-Targeted Therapies in Colon Cancer.

Cell

Zhang L,Li Z,Skrzypczynska KM,Fang Q,Zhang W,O'Brien SA,He Y,Wang L,Zhang Q,Kim A,Gao R,Orf J,Wang T,Sawant D,Kang J,Bhatt D,Lu D,Li CM,Rapaport AS,Perez K,Ye Y,Wang S,Hu X,Ren X,Ouyang W,Shen Z,Egen JG,Zhang Z,Yu X

47-0149-42 was used in Flow Cytometry to identify critical cellular interactions regulating tumor immunity and defines mechanisms underlying myeloid-targeted immunotherapies currently undergoing clinical testing.

Thu Apr 16 00:00:00 EDT 2020

Isolation of single HIV-1 Envelope specific B cells and antibody cloning from immunized rhesus macaques.

Journal of immunological methods

Wang Z,Merkenschlager J,Chen ST,Oliveira TY,Ramos V,Gordon KM,Yao KH,Jankovic M,Nussenzweig M,Escolano A

47-0149 was used in Flow cytometry/Cell sorting to describe a protocol to identify and purify single antigen-specific macaque B cells, and subsequently clone and produce macaque monoclonal antibodies.

Sun Mar 01 00:00:00 EST 2020

Intrahepatic macrophage populations in the pathophysiology of primary sclerosing cholangitis.

JHEP reports : innovation in hepatology

Chen YY,Arndtz K,Webb G,Corrigan M,Akiror S,Liaskou E,Woodward P,Adams DH,Weston CJ,Hirschfield GM

47-0149 was used in Flow cytometry/Cell sorting to show CD68+CD206+ macrophages occur in greater frequency with higher expression of TGR-5 in primary sclerosing cholangitis.

Fri Nov 01 00:00:00 EDT 2019

Modulation of B Cells and Homing Marker on NK Cells Through Extracorporeal Photopheresis in Patients With Steroid-Refractory/Resistant Graft-Vs.-Host Disease Without Hampering Anti-viral/Anti-leukemic Effects.

Frontiers in immunology

Wang L,Ni M,Hückelhoven-Krauss A,Sellner L,Hoffmann JM,Neuber B,Luft T,Hegenbart U,Schönland S,Kleist C,Sill M,Chen BA,Wuchter P,Eckstein V,Krüger W,Hilgendorf I,Yerushalmi R,Nagler A,Müller-Tidow C,Ho AD,Dreger P,Schmitt M,Schmitt A

47-0149 was used in Flow cytometry/Cell sorting to analyse the effects of extracorporeal photopheresis therapy on effector and regulatory cells in patients with graft-versus-host disease.

Mon Sep 30 00:00:00 EDT 2019

Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques.

Nature

Escolano A,Gristick HB,Abernathy ME,Merkenschlager J,Gautam R,Oliveira TY,Pai J,West AP,Barnes CO,Cohen AA,Wang H,Golijanin J,Yost D,Keeffe JR,Wang Z,Zhao P,Yao KH,Bauer J,Nogueira L,Gao H,Voll AV,Montefiori DC,Seaman MS,Gazumyan A,Silva M,McGuire AT,Stamatatos L,Irvine DJ,Wells L,Martin MA,Bjorkman PJ,Nussenzweig MC

47-0149-42 was used in Flow Cytometry to develop an immunogen, RC1, that facilitates the recognition of the variable loop 3 (V3)-glycan patch on the envelope protein of HIV-1 to activate B cells that express precursors of broadly neutralizing antibodies within polyclonal repertoires.

Sat Jun 01 00:00:00 EDT 2019

PLoS neglected tropical diseases

Narasimhan PB,Akabas L,Tariq S,Huda N,Bennuru S,Sabzevari H,Hofmeister R,Nutman TB,Tolouei Semnani R

47-0149 was used in Flow cytometry/Cell sorting to suggest that despite the fact that helminth parasites and cancer cell lines are extraordinarily disparate, they share the ability to alter the phenotype of human monocytes.

Sun Apr 01 00:00:00 EDT 2018

Surface LAMP-2 Is an Endocytic Receptor That Diverts Antigen Internalized by Human Dendritic Cells into Highly Immunogenic Exosomes.

Journal of immunology (Baltimore, Md. : 1950)

Leone DA,Peschel A,Brown M,Schachner H,Ball MJ,Gyuraszova M,Salzer-Muhar U,Fukuda M,Vizzardelli C,Bohle B,Rees AJ,Kain R

47-0149 was used in Flow cytometry/Cell sorting to identify lysosome-associated membrane protein-2 as an endocytic receptor on human monocyte-derived dendritic cells that routes cargo into unusual Ag processing pathways.

Sat Jul 15 00:00:00 EDT 2017

Enhancement of cytokine-driven NK cell IFN-γ production after vaccination of HCMV infected Africans.

European journal of immunology

Darboe A,Danso E,Clarke E,Umesi A,Touray E,Wegmuller R,Moore SE,Riley EM,Goodier MR

47-0149 was used in Flow cytometry/Cell sorting to investigate the impact of advanced NK cell differentiation on vaccine-induced responses.

Thu Jun 01 00:00:00 EDT 2017

Platelet-Derived Ectosomes Reduce NK Cell Function.

Journal of immunology (Baltimore, Md. : 1950)

Sadallah S,Schmied L,Eken C,Charoudeh HN,Amicarella F,Schifferli JA

47-0149 was used in Flow cytometry/Cell sorting to study whether platelet ectosomes modify natural killer cell function.

Thu Sep 01 00:00:00 EDT 2016

KIR/HLA interactions negatively affect rituximab- but not GA101 (obinutuzumab)-induced antibody-dependent cellular cytotoxicity.

Journal of immunology (Baltimore, Md. : 1950)

Terszowski G,Klein C,Stern M

47-0149 was used in Flow cytometry/Cell sorting to show that modification of the Fc fragment to enhance ADCC can be an effective strategy to augment the efficacy of therapeutic mAbs by recruiting NK cells irrespective of their inhibitory KIR expression.

Sun Jun 15 00:00:00 EDT 2014

The strength of the antibody response to the nematode Ascaris lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF).

BMC immunology

Bornacelly A,Mercado D,Acevedo N,Caraballo L

47-0149 was used in Flow cytometry/Cell sorting to suggest that differences in B-Cell Activating Factor levels are related to the strength of the antibody response to Ascaris.

Sat Jun 07 00:00:00 EDT 2014

Human female genital tract infection by the obligate intracellular bacterium Chlamydia trachomatis elicits robust Type 2 immunity.

PloS one

Vicetti Miguel RD,Harvey SA,LaFramboise WA,Reighard SD,Matthews DB,Cherpes TL

47-0149 was used in Flow cytometry/Cell sorting to assess changes in the co-expression of CD200 and CD206 by endometrial macrophages during upper genital tract Chlamydia infection.

Mon Sep 30 00:00:00 EDT 2013

Plasmacytoid dendritic cells and C1q differentially regulate inflammatory gene induction by lupus immune complexes.

Journal of immunology (Baltimore, Md. : 1950)

Santer DM,Wiedeman AE,Teal TH,Ghosh P,Elkon KB

47-0149 was used in Flow cytometry/Cell sorting to investigate the dominant blood cell types and inflammation-related gene programs stimulated by lupus immune complexes.

Sun Jan 15 00:00:00 EST 2012

Bioinformatics

Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

Gene Aliases: CD14

UniProt ID: (Human) P08571

Entrez Gene ID: (Human) 929

Molecular Function: transmembrane signal receptor

Function(s)

MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway receptor-mediated endocytosis phagocytosis apoptotic process inflammatory response cell surface receptor signaling pathway I-kappaB kinase/NF-kappaB signaling response to heat lipopolysaccharide-mediated signaling pathway response to magnesium ion positive regulation of type I interferon production positive regulation of interferon-gamma production positive regulation of tumor necrosis factor production negative regulation of MyD88-independent toll-like receptor signaling pathway toll-like receptor 4 signaling pathway response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway innate immune response response to ethanol positive regulation of endocytosis positive regulation of cytokine secretion response to electrical stimulus necroptotic process cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide apoptotic signaling pathway

Process(es)

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding

CD14 Monoclonal Antibody (61D3), APC-eFluor™ 780, eBioscience™

Product Details

47-0149-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

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