|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Tthe CD14 antigen|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Optimization of the cytokine secretion assay for human IL-2 in single and combination assays.
Q10064 was used in flow cytometry to optimize detection of IL-2 from live cells
|Deng N,Mosmann TR||Cytometry. Part A : the journal of the International Society for Analytical Cytology (87:777)||2015|
Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody.
Q10064 was used in flow cytometry to examine various DC after flu vaccination.
|Kobie JJ,Treanor JJ,Ritchlin CT||Immunological investigations (43:606)||2014|
Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination.
Q10064 was used in flow cytometry to study influenza-specific CD4 T cells respond to vaccination using Ki67 as a marker.
|Li X,Miao H,Henn A,Topham DJ,Wu H,Zand MS,Mosmann TR||Vaccine (30:4581)||2012|
CD8+ T cell immunity to 2009 pandemic and seasonal H1N1 influenza viruses.
Q10064 was used in flow cytometry to examine human cross-reactive T cells against a pandemic virus.
|Scheible K,Zhang G,Baer J,Azadniv M,Lambert K,Pryhuber G,Treanor JJ,Topham DJ||Vaccine (29:2159)||2011|