|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2a, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.
Applications Reported: This APA5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
||Olig2+ neuroepithelial motoneuron progenitors are not multipotent stem cells in vivo.||Mukouyama YS,Deneen B,Lukaszewicz A,Novitch BG,Wichterle H,Jessell TM,Anderson DJ||Proceedings of the National Academy of Sciences of the United States of America (103:1551)||2006|
|Not Applicable||Not Cited||Tlx, an orphan nuclear receptor, regulates cell numbers and astrocyte development in the developing retina.||Miyawaki T,Uemura A,Dezawa M,Yu RT,Ide C,Nishikawa S,Honda Y,Tanabe Y,Tanabe T||The Journal of neuroscience : the official journal of the Society for Neuroscience (24:8124)||2004|
|Not Applicable||Not Cited||Olig2+ neuroepithelial motoneuron progenitors are not multipotent stem cells in vivo.||Mukouyama YS,Deneen B,Lukaszewicz A,Novitch BG,Wichterle H,Jessell TM,Anderson DJ||Proceedings of the National Academy of Sciences of the United States of America (103:1551)||2006|
|Not Applicable||Not Cited||PDGFR alpha expression during mouse embryogenesis: immunolocalization analyzed by whole-mount immunohistostaining using the monoclonal anti-mouse PDGFR alpha antibody APA5.||Takakura N,Yoshida H,Ogura Y,Kataoka H,Nishikawa S,Nishikawa S||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (45:883)||1997|