|Tested species reactivity||Human|
|Host / Isotype||Sheep / IgG|
|Immunogen||Human Tissue Factor.|
|Purification||Ion-exchange chromatography, Ammonium sulfate precipitation|
|Storage buffer||50% water with 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||5-10 µg/mL|
|Western Blot (WB)||5-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-43058 detects Tissue Factor in human samples.
PA1-43058 has successfully been used in Western blot and ELISA applications.
PA1-43058 was derived from human tissue factor.
This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.