Invitrogen

CD144 (VE-cadherin) Monoclonal Antibody (16B1), Biotin, eBioscience™

Product Image Gallery

CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow) — Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.25 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.125 µg of Anti-Human CD144 (VE-Cadherin) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD144 (VE-cadherin) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Fig 1 Circulating Tumour Cell (CTC) detected by Immunocytochemical (ICC)-Staining of ISET Filters. Large cells from blood samples are enriched by ISET filtration and immunocytochemically stained for the White Blood Cell marker CD45 and the Endothelial Cell marker CD144 (yellow arrow). Circulating Tumour Cells (CTCs) are identified as >=16mum diameter cells with a hyperchromatic nucleus and negative for CD45/CD144 brown chromogen staining (blue arrowhead). The dark circles are 8mum filter pores (black arrow).

CD144 (VE-cadherin) Antibody in Immunocytochemistry (ICC/IF) — Figure 6 Single-cell CNA analysis of VE-cadherin-negative and -positive CTCs from an ES SCLC patient. ( a ) CTCs identified using the HD-SCA assay: cells were stained with DAPI (white) and antibodies to CKs (green), CD45 (red) and VE-cadherin (blue) and pseudo-coloured. Top panel, DAPI +VE /CD45 -VE /CK +VE /VE-cadherin +VE CTC; middle panel, DAPI +VE /CD45 -VE /CK +VE /VE-cadherin -VE CTC; bottom panel, DAPI +VE /CD45 +VE /CK -VE /VE-cadherin -VE white blood cell (WBC). Arrows link to the respective CNA analysis of the indicated cell, showing loss of 3p, gain of 5p including TERT and hemizygous loss of RB1 on 13 in CTC. Major chromosome losses (blue arrow) and gains (red arrow) highlighted above. The selected WBC in contrast has a characteristic flat CNA profile indicative of healthy somatic cells. Representative images and profiles are shown. Scaled x 10 images from the scanner are shown for CD45, while remaining images were acquired at x 40. Scale bar, 10 mum. ( b ) CNA analysis of matched patient ctDNA: the GC-normalized and mappability corrected read counts (log 2 scale) were segmented using Hidden Markov Model (HMM), red=copy-number gains, blue=copy-number losses. Major chromosome losses (blue arrow) and gains (red arrow) highlighted above and match pattern identified by single CTC CNA.

CD144 (VE-cadherin) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunocytochemistry and Flow Cytometry

CD144 (VE-cadherin) Antibody in Immunohistochemistry (IHC) — Figure 7 Functional significance of VE-cadherin expression for SCLC VM in vitro and in vivo . ( a ) Western blot analysis of VE-cadherin expression levels in VM proficient C8161 melanoma cells and H446, H446 non-silencing empty vector controls, H446 VE-cadherin shRNA knockdown and H1048 SCLC cells. ( b ) VM-like network formation on matrigel for VE-cadherin +VE cells (C8161, H446 and H446 E.V) and VE-cadherin -VE cells (H1048 and H446 VE-cadherin KD) lacking network formation. Representative images are shown for n >3 experiments. Scale bar, 200 mum. ( c ) Left panel, representative images of anti-human anti-VE-cadherin staining in H446 parental and H446 VE-cadherin KD xenografts. Scale bar, 50 mum. ( c ) Right panel percentage of cells positive for VE-cadherin in H446 and H446 VE-cadherin KD xenografts (*** P =0.0001, two-tailed t -test), n =10 animals per group. ( d ) Western blot analysis VE-cadherin expression in H446 and H446 VE-cadherin KD xenografts ( n =6 tumours for each group). ( e ) Left panel, representative images of anti-mouse anti-CD31/PAS staining in H446 Parental and H446 VE-cadherin KD tumours. Scale bars, 50 mum. ( e ) Right panel VM ratio in H446 parental ( n =10) and H446 VE-cadherin KD xenografts ( n =8; *** P =0.0005, two-tailed Mann-Whitney's test). ( f ) Tumour growth rates in H446 (black) and H446 VE-cadherin KD (grey) tumours ( n =10 animals per group). ( g ) Days to 200 mm 3 volume tumours in H446 and H446 VE-cadherin xenografts KD ( n =20 anim

Product Details

13-1449-82

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunocytochemistry (ICC/IF)	Assay-Dependent	View 5 publications 5 publications
Flow Cytometry (Flow)	0.125 µg/test	View 7 publications 7 publications

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	16B1
Conjugate	Biotin Alexa Fluor 488 Alexa Fluor 700 APC APC-eFluor 780 PE PE-Cyanine7 PE-eFluor 610 Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_466611

Product Specific Information

Description: The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.

Applications Reported: This 16B1 antibody has been reported for use in flow cytometric analysis, and immunocytochemistry.

Applications Tested: This 16B1 antibody has been tested by flow cytometric analysis of Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting QKI-7 in vivo restores endothelial cell function in diabetes.

Nature communications

Yang C,Eleftheriadou M,Kelaini S,Morrison T,González MV,Caines R,Edwards N,Yacoub A,Edgar K,Moez A,Ivetic A,Zampetaki A,Zeng L,Wilkinson FL,Lois N,Stitt AW,Grieve DJ,Margariti A

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2020

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem cell reports

Li Y,Brauer PM,Singh J,Xhiku S,Yoganathan K,Zúñiga-Pflücker JC,Anderson MK

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Tue Sep 12 00:00:00 EDT 2017

ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells.

Stem cell research & therapy

Almalki SG,Agrawal DK

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Fri May 12 00:00:00 EDT 2017

MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells.

Stem cells translational medicine

Almalki SG,Llamas Valle Y,Agrawal DK

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

Challenges in enumeration of CTCs in breast cancer using techniques independent of cytokeratin expression.

PloS one

Castle J,Morris K,Pritchard S,Kirwan CC

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunocytochemistry

Thu Apr 27 00:00:00 EDT 2017

Challenges in enumeration of CTCs in breast cancer using techniques independent of cytokeratin expression.

PloS one

Castle J,Morris K,Pritchard S,Kirwan CC

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunocytochemistry

Thu Apr 27 00:00:00 EDT 2017

Vasculogenic mimicry in small cell lung cancer.

Nature communications

Williamson SC,Metcalf RL,Trapani F,Mohan S,Antonello J,Abbott B,Leong HS,Chester CP,Simms N,Polanski R,Nonaka D,Priest L,Fusi A,Carlsson F,Carlsson A,Hendrix MJ,Seftor RE,Seftor EA,Rothwell DG,Hughes A,Hicks J,Miller C,Kuhn P,Brady G,Simpson KL,Blackhall FH,Dive C

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunohistochemistry

Wed Nov 09 00:00:00 EST 2016

Vasculogenic mimicry in small cell lung cancer.

Nature communications

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunohistochemistry

Wed Nov 09 00:00:00 EST 2016

Tumourigenic non-small-cell lung cancer mesenchymal circulating tumour cells: a clinical case study.

Annals of oncology : official journal of the European Society for Medical Oncology

Morrow CJ,Trapani F,Metcalf RL,Bertolini G,Hodgkinson CL,Khandelwal G,Kelly P,Galvin M,Carter L,Simpson KL,Williamson S,Wirth C,Simms N,Frankliln L,Frese KK,Rothwell DG,Nonaka D,Miller CJ,Brady G,Blackhall FH,Dive C

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunofluorescence

Wed Jun 01 00:00:00 EDT 2016

Tumourigenic non-small-cell lung cancer mesenchymal circulating tumour cells: a clinical case study.

Annals of oncology : official journal of the European Society for Medical Oncology

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Immunofluorescence

Wed Jun 01 00:00:00 EDT 2016

Rab11a Mediates Vascular Endothelial-Cadherin Recycling and Controls Endothelial Barrier Function.

Arteriosclerosis, thrombosis, and vascular biology

Yan Z,Wang ZG,Segev N,Hu S,Minshall RD,Dull RO,Zhang M,Malik AB,Hu G

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Rab11a Mediates Vascular Endothelial-Cadherin Recycling and Controls Endothelial Barrier Function.

Arteriosclerosis, thrombosis, and vascular biology

Yan Z,Wang ZG,Segev N,Hu S,Minshall RD,Dull RO,Zhang M,Malik AB,Hu G

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Direct conversion of human amniotic cells into endothelial cells without transitioning through a pluripotent state.

Nature protocols

Ginsberg M,Schachterle W,Shido K,Rafii S

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Direct conversion of human amniotic cells into endothelial cells without transitioning through a pluripotent state.

Nature protocols

Ginsberg M,Schachterle W,Shido K,Rafii S

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Histone Demethylases KDM4A and KDM4C Regulate Differentiation of Embryonic Stem Cells to Endothelial Cells.

Stem cell reports

Wu L,Wary KK,Revskoy S,Gao X,Tsang K,Komarova YA,Rehman J,Malik AB

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1449-82) in Flow Cytometry

Tue Jul 14 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: 7B4 antigen; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; Vascular endothelial cadherin; VE-cadherin

Gene Aliases: 7B4; CD144; CDH5

UniProt ID: (Human) P33151

Entrez Gene ID: (Human) 1003

Molecular Function: cadherin