Invitrogen

CD144 (VE-cadherin) Monoclonal Antibody (16B1), eBioscience™

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated. View Details

32 Published Figures

34 References

View all (41) VE-cadherin (CD144) antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

CD144 (VE-cadherin) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of VE-cadherin was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD144 (VE-cadherin) Monoclonal Antibody (16B1), eBioscience™ (Product # 14-1449-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing plasma membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CD144 (VE-cadherin) Antibody in Western Blot (WB) — EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were resolved by SDS -PAGE and immunoblotted with 2 µg/mL of Anti-Human CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP.

CD144 (VE-cadherin) Antibody — Functional significance of VE-cadherin expression for SCLC VM in vitro and in vivo. (a) Western blot analysis of VE-cadherin expression levels in VM proficient C8161 melanoma cells and H446, H446 non-silencing empty vector controls, H446 VE-cadherin shRNA knockdown and H1048 SCLC cells. (b) VM-like network formation on matrigel for VE-cadherin+VE cells (C8161, H446 and H446 E.V) and VE-cadherin−VE cells (H1048 and H446 VE-cadherin KD) lacking network formation. Representative images are shown for n>3 experiments. Scale bar, 200 μm. (c) Left panel, representative images of anti-human anti-VE-cadherin staining in H446 parental and H446 VE-cadherin KD xenografts. Scale bar, 50 μm. (c) Right panel percentage of cells positive for VE-cadherin in H446 and H446 VE-cadherin KD xenografts (***P=0.0001, two-tailed t-test), n=10 animals per group. (d) Western blot analysis VE-cadherin expression in H446 and H446 VE-cadherin KD xenografts (n=6 tumours for each group). (e) Left panel, representative images of anti-mouse anti-CD31/PAS staining in H446 Parental and H446 VE-cadherin KD tumours. Scale bars, 50 μm. (e) Right panel VM ratio in H446 parental (n=10) and H446 VE-cadherin KD xenografts (n=8; ***P=0.0005, two-tailed Mann–Whitney's test). (f) Tumour growth rates in H446 (black) and H446 VE-cadherin KD (grey) tumours (n=10 animals per group). (g) Days to 200 mm3 volume tumours in H446 and H446 VE-cadherin xenografts KD (n=20 animals per group, (***P<0.0001, two-tailed t-test). Error b {KD}

CD144 (VE-cadherin) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Fig 1 Circulating Tumour Cell (CTC) detected by Immunocytochemical (ICC)-Staining of ISET Filters. Large cells from blood samples are enriched by ISET filtration and immunocytochemically stained for the White Blood Cell marker CD45 and the Endothelial Cell marker CD144 (yellow arrow). Circulating Tumour Cells (CTCs) are identified as >=16mum diameter cells with a hyperchromatic nucleus and negative for CD45/CD144 brown chromogen staining (blue arrowhead). The dark circles are 8mum filter pores (black arrow).

CD144 (VE-cadherin) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 14-1449-82) in Western Blot and Immunocytochemistry

CD144 (VE-cadherin) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 14-1449-82) in Immunocytochemistry and Flow Cytometry

CD144 (VE-cadherin) Antibody in Immunohistochemistry (IHC) — Fig 3 False positive CTCs identified by ISET filtration and morphology criteria in healthy volunteers. Presumed false positive ''CTC'' (blue arrow head). Cell staining positive for CD45/CD144, consistent with white blood cell or endothelial cell (yellow arrow). The dark circles are 8mum filter pores (black arrow).

CD144 (VE-cadherin) Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 7 Functional significance of VE-cadherin expression for SCLC VM in vitro and in vivo . ( a ) Western blot analysis of VE-cadherin expression levels in VM proficient C8161 melanoma cells and H446, H446 non-silencing empty vector controls, H446 VE-cadherin shRNA knockdown and H1048 SCLC cells. ( b ) VM-like network formation on matrigel for VE-cadherin +VE cells (C8161, H446 and H446 E.V) and VE-cadherin -VE cells (H1048 and H446 VE-cadherin KD) lacking network formation. Representative images are shown for n >3 experiments. Scale bar, 200 mum. ( c ) Left panel, representative images of anti-human anti-VE-cadherin staining in H446 parental and H446 VE-cadherin KD xenografts. Scale bar, 50 mum. ( c ) Right panel percentage of cells positive for VE-cadherin in H446 and H446 VE-cadherin KD xenografts (*** P =0.0001, two-tailed t -test), n =10 animals per group. ( d ) Western blot analysis VE-cadherin expression in H446 and H446 VE-cadherin KD xenografts ( n =6 tumours for each group). ( e ) Left panel, representative images of anti-mouse anti-CD31/PAS staining in H446 Parental and H446 VE-cadherin KD tumours. Scale bars, 50 mum. ( e ) Right panel VM ratio in H446 parental ( n =10) and H446 VE-cadherin KD xenografts ( n =8; *** P =0.0005, two-tailed Mann-Whitney's test). ( f ) Tumour growth rates in H446 (black) and H446 VE-cadherin KD (grey) tumours ( n =10 animals per group). ( g ) Days to 200 mm 3 volume tumours in H446 and H446 VE-cadherin xenografts KD ( n =20 anim

CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow) — Fig. 3 Human iPS cells differentiation toward ECs. Morphology of hiPSCs and their EC differentiated counterparts are shown by bright field microscopy. Scale bar: 50 mum ( a ). Flow cytometry showed the pure population of hiPS-derived ECs after MACS selection using CD144 magnetic beads ( b ). Immunofluorescence confocal image showing that the differentiated ECs expressed the EC-specific markers CD31, CD144, and ZO-1 localizing to cell-cell junction. QKI-7 displayed perinuclear cytoplasm localization. Scale bar: 25 mum ( c ). The expression of EC marker proteins CD31, CD144, KDR, and eNOS was shown by western blot ( d ). hiPS-ECs formed tube structure indicating their angiogenic capacity. Scale bar: 200 mum ( e ). Data are from n = 3 representative images. Source data are provided as a Source data file.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 3 publications 3 publications

Immunohistochemistry (IHC)

Assay-Dependent

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

View 8 publications 8 publications

Flow Cytometry (Flow)

Assay-Dependent

View 17 publications 17 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

16B1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_467495

Product Specific Information

Description: The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.

Applications Reported: This 16B1 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), microscopy, and immunocytochemistry without fixation.

Applications Tested: This 16B1 antibody has been tested by immunoblotting Human Umbilical Vein Endothelial Cell (HUVEC) lysates. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 7B4 antigen; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; Vascular endothelial cadherin; VE-cadherin

Gene Aliases: 7B4; CD144; CDH5

UniProt ID: (Human) P33151

Entrez Gene ID: (Human) 1003

Molecular Function: cadherin