Invitrogen

CD144 (VE-cadherin) Monoclonal Antibody (16B1), eBioscience™

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated. View Details

21 Published Figures

33 References

View all (41) VE-cadherin (CD144) antibodies

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Questions & Answers

Datasheet

Questions & Answers

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CD144 (VE-cadherin) Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of VE-cadherin was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD144 (VE-cadherin) Monoclonal Antibody (16B1), eBioscience™ (Product # 14-1449-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing plasma membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CD144 (VE-cadherin) Antibody in Western Blot (WB) — EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were resolved by SDS -PAGE and immunoblotted with 2 µg/mL of Anti-Human CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP.

CD144 (VE-cadherin) Antibody in Independent antibody — Antibody specificity was demonstrated by showing that antibodies raised against the same target protein perform similarly. Immunofluorescence of VE-cadherin using CD144 (VE-cadherin) Monoclonal Antibody (16B1) (Product # 14-1449-82) performed along with another VE-cadherin Polyclonal Antibody (Product # PA5-17401) shows similar localization pattern in HUVEC cells. {IAV}

CD144 (VE-cadherin) Antibody — Figure 6 Ectopic Expression of HEBCan in HEB -/- hESCs Restores Lineage-Specific Gene Expression and Hematopoietic Specification (A) Western blot analysis for HEB expression in WT, KO (HEB -/- ), KO + GFP (HEB -/- hESCs transduced with GFP control vector) and KO + HEBCan (HEB -/- hESCs transduced with HEBCan-encoding vector) hESCs. (B) Bright-field (top) and fluorescent (bottom) images of day-8 (d8) EBs derived from HEB -/- hESCs transduced with control or HEBCan-expressing lentiviral particles. Scale bar, 100 mum. (C and D) qRT-PCR analysis for the expression of pluripotency-associated genes (C) and mesoendodermal genes (D) in WT, KO + GFP, and KO + HEBCan hESC-derived cells at d0 and d4 of EB culture. mRNA levels are shown relative to GAPDH. (E) Flow-cytometric analysis of CD34 and KDR, CD144, and CD31 on WT, KO + GFP, and KO + HEBCan d8 EB-derived cells. (F and G) Percentages (F) and numbers (G) of CD34 + cells in WT, KO + GFP, and KO + HEBCan d8 EBs. (H) Flow-cytometric analysis for CD34 and CD45 on WT, KO + GFP, and KO + HEBCan d18 EB-derived cells. (I and J) Percentages (I) and numbers (J) of CD34/CD45 subsets in WT, KO + GFP, and KO + HEBCan d18 EB-derived cells. (K) Numbers of erythroid (BFU-E) and myeloid (CFU-GM) arising from unfractionated WT, KO + GFP, and KO + HEBCan d18 EBs. Error bars represent mean +- SD (n = 3 independent experiments). * p < 0.05, ** p < 0.01, *** p < 0.005 by Student and apos;s t test. Images in (A) and (B) and plots in (E) and (H) are represent

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 9 publications 9 publications

Immunocytochemistry (ICC)

5 µg/mL

View 6 publications 6 publications

Immunofluorescence (IF)

5 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

Assay-Dependent

View 5 publications 5 publications

Western Blot (WB)

Assay-Dependent

View 2 publications 2 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

16B1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_467495

Product Specific Information

Description: The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.

Applications Reported: This 16B1 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), microscopy, and immunocytochemistry without fixation.

Applications Tested: This 16B1 antibody has been tested by immunoblotting Human Umbilical Vein Endothelial Cell (HUVEC) lysates. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 7B4 antigen; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; Vascular endothelial cadherin; VE-cadherin

Gene Aliases: 7B4; CD144; CDH5

UniProt ID: (Human) P33151

Entrez Gene ID: (Human) 1003

Molecular Function: cadherin