Invitrogen

CD144 (VE-cadherin) Monoclonal Antibody (16B1), Alexa Fluor™ 700, eBioscience™

Product Image Gallery

CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow) — Human Umbilical Endothelial Cells (HUVEC) were stained with Mouse IgG1 kappa Isotype Control, Alexa Fluor 700 (Product # 56-4714-80) (blue histogram) or CD144 (VE-cadherin) Monoclonal Antibody, Alexa Fluor 700 (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).

CD144 (VE-cadherin) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Fig 1 Circulating Tumour Cell (CTC) detected by Immunocytochemical (ICC)-Staining of ISET Filters. Large cells from blood samples are enriched by ISET filtration and immunocytochemically stained for the White Blood Cell marker CD45 and the Endothelial Cell marker CD144 (yellow arrow). Circulating Tumour Cells (CTCs) are identified as >=16mum diameter cells with a hyperchromatic nucleus and negative for CD45/CD144 brown chromogen staining (blue arrowhead). The dark circles are 8mum filter pores (black arrow).

CD144 (VE-cadherin) Antibody in Immunocytochemistry (ICC/IF) — Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Immunocytochemistry

CD144 (VE-cadherin) Antibody in Immunohistochemistry (IHC) — Figure 2 Generation and characterization of endothelial-specific YY1 deficient mice for the tumor angiogenesis. ( A ) Schematic diagram for the endothelial cell specific deletion of YY1 in mice (Ve-Cad-CreER T2 ; YY1 flox/flox , YY1 iDeltaEC ) and the strategy of the tumor angiogenesis study. The tumor was induced by melanoma B16-F10 cells (5 x 10 cells per mouse) with subcutaneously transplanted into 8-week-old WT or YY1 iEC mice. ( B ) PCR analysis for the genotyping of Ve-Cad-CreER T2 ; YY1 flox/flox mice (YY1 iDeltaEC ) and YY1 flox/flox (WT) mice. ( C ) Immunofluorescence image of endothelial cell marker VE-Cadherin in lung endothelial cells isolated from YY1 iDeltaEC mice. Nuclei were labeled by DAPI (blue) (Left panel) and Western blot analysis of endothelial YY1 expression in mouse lung endothelial cells isolated from WT and YY1 iDeltaEC mice (n = 3) (Right panel). ( D ) Dual immunostaining analysis of YY1 (red) and endothelial cell marker CD31 (green) in melanoma tumor tissues isolated from WT and YY1 iDeltaEC mice, (n = 7). Scale bars: 20 mum.

Product Details

56-1449-42

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	5 µL (0.25 µg)/test	View 13 publications 13 publications

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Mouse / IgG1
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	16B1
Conjugate	Alexa Fluor™ 700 Alexa Fluor 488 APC APC-eFluor 780 Biotin PE PE-Cyanine7 PE-eFluor 610 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_11042329

Product Specific Information

Description: The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.

Applications Reported: This 16B1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 16B1 antibody has been pre-diluted and tested by flow cytometric analysis of Human Umbilical Vein Endothelial Cells (HUVEC). This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser

Target Information

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Endothelial specific YY1 deletion restricts tumor angiogenesis and tumor growth.

Scientific reports

Liu H,Qiu Y,Pei X,Chitteti R,Steiner R,Zhang S,Jin ZG

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Immunohistochemistry

Tue Nov 24 00:00:00 EST 2020

Targeting QKI-7 in vivo restores endothelial cell function in diabetes.

Nature communications

Yang C,Eleftheriadou M,Kelaini S,Morrison T,González MV,Caines R,Edwards N,Yacoub A,Edgar K,Moez A,Ivetic A,Zampetaki A,Zeng L,Wilkinson FL,Lois N,Stitt AW,Grieve DJ,Margariti A

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2020

Isolating pulmonary microvascular endothelial cells ex vivo: Implications for pulmonary arterial hypertension, and a caution on the use of commercial biomaterials.

PloS one

Wertheim BM,Lin YD,Zhang YY,Samokhin AO,Alba GA,Arons E,Yu PB,Maron BA

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Flow Cytometry

Mon Nov 04 00:00:00 EST 2019

Enhanced Therapeutic and Long-Term Dynamic Vascularization Effects of Human Pluripotent Stem Cell-Derived Endothelial Cells Encapsulated in a Nanomatrix Gel.

Circulation

Lee SJ,Sohn YD,Andukuri A,Kim S,Byun J,Han JW,Park IH,Jun HW,Yoon YS

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Flow Cytometry

Tue Nov 14 00:00:00 EST 2017

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem cell reports

Li Y,Brauer PM,Singh J,Xhiku S,Yoganathan K,Zúñiga-Pflücker JC,Anderson MK

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Flow Cytometry

Tue Sep 12 00:00:00 EDT 2017

ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells.

Stem cell research & therapy

Almalki SG,Agrawal DK

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Flow Cytometry

Fri May 12 00:00:00 EDT 2017

MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells.

Stem cells translational medicine

Almalki SG,Llamas Valle Y,Agrawal DK

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

Challenges in enumeration of CTCs in breast cancer using techniques independent of cytokeratin expression.

PloS one

Castle J,Morris K,Pritchard S,Kirwan CC

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Immunocytochemistry

Thu Apr 27 00:00:00 EDT 2017

Challenges in enumeration of CTCs in breast cancer using techniques independent of cytokeratin expression.

PloS one

Castle J,Morris K,Pritchard S,Kirwan CC

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Immunocytochemistry

Thu Apr 27 00:00:00 EDT 2017

Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells.

Nature protocols

Palpant NJ,Pabon L,Friedman CE,Roberts M,Hadland B,Zaunbrecher RJ,Bernstein I,Zheng Y,Murry CE

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Vasculogenic mimicry in small cell lung cancer.

Nature communications

Williamson SC,Metcalf RL,Trapani F,Mohan S,Antonello J,Abbott B,Leong HS,Chester CP,Simms N,Polanski R,Nonaka D,Priest L,Fusi A,Carlsson F,Carlsson A,Hendrix MJ,Seftor RE,Seftor EA,Rothwell DG,Hughes A,Hicks J,Miller C,Kuhn P,Brady G,Simpson KL,Blackhall FH,Dive C

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Immunohistochemistry

Wed Nov 09 00:00:00 EST 2016

Rab11a Mediates Vascular Endothelial-Cadherin Recycling and Controls Endothelial Barrier Function.

Arteriosclerosis, thrombosis, and vascular biology

Yan Z,Wang ZG,Segev N,Hu S,Minshall RD,Dull RO,Zhang M,Malik AB,Hu G

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Induced Pluripotent Stem Cells to Model Human Fibrodysplasia Ossificans Progressiva.

Stem cell reports

Cai J,Orlova VV,Cai X,Eekhoff EMW,Zhang K,Pei D,Pan G,Mummery CL,Ten Dijke P

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2015

Direct conversion of human amniotic cells into endothelial cells without transitioning through a pluripotent state.

Nature protocols

Ginsberg M,Schachterle W,Shido K,Rafii S

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Histone Demethylases KDM4A and KDM4C Regulate Differentiation of Embryonic Stem Cells to Endothelial Cells.

Stem cell reports

Wu L,Wary KK,Revskoy S,Gao X,Tsang K,Komarova YA,Rehman J,Malik AB

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Flow Cytometry

Tue Jul 14 00:00:00 EDT 2015

Efficient direct reprogramming of mature amniotic cells into endothelial cells by ETS factors and TGFβ suppression.

Cell

Ginsberg M,James D,Ding BS,Nolan D,Geng F,Butler JM,Schachterle W,Pulijaal VR,Mathew S,Chasen ST,Xiang J,Rosenwaks Z,Shido K,Elemento O,Rabbany SY,Rafii S

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Flow Cytometry

Fri Oct 26 00:00:00 EDT 2012

Identification of the hemogenic endothelial progenitor and its direct precursor in human pluripotent stem cell differentiation cultures.

Cell reports

Choi KD,Vodyanik MA,Togarrati PP,Suknuntha K,Kumar A,Samarjeet F,Probasco MD,Tian S,Stewart R,Thomson JA,Slukvin II

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 56-1449-42) in Flow Cytometry

Thu Sep 27 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: 7B4 antigen; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; Vascular endothelial cadherin; VE-cadherin

Gene Aliases: 7B4; CD144; CDH5

UniProt ID: (Human) P33151

Entrez Gene ID: (Human) 1003

Molecular Function: cadherin