|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2a, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.125 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 3 publications below|
Description: The RL73 monoclonal antibody reacts with mouse CD147, also known as Basigin. CD147 is expressed by a majority of thymocytes, activated peripheral T cells, erythrocytes and erythroblasts. It has been reported that CD147 expression correlates with cycling of immature thymocytes even in the absence of TCRbeta selection and that ligation of this molecule on immature fetal thymocytes inhibits their further development into mature T cells.
Applications Reported: The RL73 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RL73 antibody has been tested by flow cytometric analysis of mouse thymocyte cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||A role for CD147 in thymic development.||Renno T,Wilson A,Dunkel C,Coste I,Maisnier-Patin K,Benoit de Coignac A,Aubry JP,Lees RK,Bonnefoy JY,MacDonald HR,Gauchat JF||Journal of immunology (Baltimore, Md. : 1950) (168:4946)||2002|
|Not Applicable||Not Cited||Unavailability of CD147 leads to selective erythrocyte trapping in the spleen.||Coste I,Gauchat JF,Wilson A,Izui S,Jeannin P,Delneste Y,MacDonald HR,Bonnefoy JY,Renno T||Blood (97:3984)||2001|
|Not Applicable||Not Cited||Cell surface glycoproteins involved in the stimulation of interleukin 1-dependent interleukin 2 production by a subline of EL4 thymoma cells. I. Functional characterization by monoclonal antibodies.||MacDonald HR,Lees RK,Bron C||Journal of immunology (Baltimore, Md. : 1950) (135:3944)||1985|