Immunofluorescent analysis of CD147/BSG was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD147/BSG Rabbit Polyclonal Antibody (34-5600) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminal region of the human EMMPRIN protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 3 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 1 publications below|
CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD147-targeted siRNA in A375 malignant melanoma cells induces the phosphorylation of EGFR and downregulates cdc25C and MEK phosphorylation.
34-5600 was used in western blot to analyze the induction of the phosphorylation of EGFR and downregulation of cdc25C and MEK phosphorylation by CD147-targeted siRNA in A375 malignant melanoma cells
|Hatanaka M,Higashi Y,Kawai K,Su J,Zeng W,Chen X,Kanekura T||Oncology letters (11:2424)||2016|
|Human||Not Cited||Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migration.||Venkatesan B,Valente AJ,Reddy VS,Siwik DA,Chandrasekar B||American journal of physiology. Heart and circulatory physiology (297:H874)||2009|
|Not Applicable||Not Cited||
EMMPRIN modulates migration and deposition of TN-C in oral squamous carcinoma.
34-5600 was used in western blot to investigate the contribution of extracellular matrix metalloproteinase inducer to tumor development
|Dang D,Atakilit A,Ramos DM||Anticancer research (28:2049)||2008|
EMMPRIN co-expressed with matrix metalloproteinases predicts poor prognosis in patients with osteosarcoma.
34-5600 was used in immunohistochemistry - paraffin section to evaluate the prognostic significance of EMMPRIN and matrix metalloproteinases for osteosarcoma
|Futamura N,Nishida Y,Urakawa H,Kozawa E,Ikuta K,Hamada S,Ishiguro N||Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (35:5159)||2014|
Renal cell neoplasias: reversion-inducing cysteine-rich protein with Kazal motifs discriminates tumor subtypes, while extracellular matrix metalloproteinase inducer indicates prognosis.
34-5600 was used in immunohistochemistry - paraffin section and western blot to study reversion-inducing cysteine-rich protein with Kazal motifs and the extracellular matrix metalloproteinase inducer in renal cell cancer.
|Rabien A,Stephan C,Kilic E,Weichert W,Kristiansen G,Miller K,Jung K,Erbersdobler A||Journal of translational medicine (11:null)||2013|
Multi-photon imaging of tumor cell invasion in an orthotopic mouse model of oral squamous cell carcinoma.
34-5600 was used in immunohistochemistry - paraffin section to describe a protocol that allows multi-vectorial visualization of lingual tumor spread.
|Gatesman Ammer A,Hayes KE,Martin KH,Zhang L,Spirou GA,Weed SA||Journal of visualized experiments : JoVE (null:null)||2011|
Extracellular matrix metalloproteinase inducer shows active perivascular cuffs in multiple sclerosis.
34-5600 was used in immunohistochemistry - frozen section to examine the expression of extracellular matrix metalloproteinase inducer in murine experimental autoimmune encephalomyelitis and multiple sclerosis specimens.
|Agrawal SM,Williamson J,Sharma R,Kebir H,Patel K,Prat A,Yong VW||Brain : a journal of neurology (136:1760)||2013|
High extracellular matrix metalloproteinase inducer/CD147 expression is strongly and independently associated with poor prognosis in colorectal cancer.
34-5600 was used in immunohistochemistry to examine expression of extracellular matrix metalloproteinase inducer in patients with colorectal cancer.
|Stenzinger A,Wittschieber D,von Winterfeld M,Goeppert B,Kamphues C,Weichert W,Dietel M,Rabien A,Klauschen F||Human pathology (43:1471)||2012|
|Human||Not Cited||Decreased RECK and Increased EMMPRIN expression in urothelial carcinoma of the bladder are associated with tumor aggressiveness.||Wittschieber D,Stenzinger A,Klauschen F,Stephan C,Jung K,Erbersdobler A,Rabien A||Pathobiology : journal of immunopathology, molecular and cellular biology (78:123)||2011|