Invitrogen

CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), APC, eBioscience™

Product Image Gallery

CD152 (CTLA-4) Antibody in Flow Cytometry (Flow) — C57Bl/6 splenocytes were unstimulated (left) or stimulated with Con A for 3 days (right). Cells were intracellularly stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0041-82) and with 0.5 µg of Armenian Hamster IgG Isotype Control APC (Product # 17-4888-82) (blue histogram) or 0.5 µg of Anti-Mouse CD152 (CTLA-4) APC (purple histogram) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the CD4+ gate were used for analysis.

Product Details

17-1522-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	1 µg/Test	View 23 publications 23 publications

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Armenian hamster / IgG
Recommended Isotype Control	Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	UC10-4B9
Conjugate	APC Biotin PE PE-Cyanine7 PE-eFluor 610 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2016700

Product Specific Information

Description: The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells at low level and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly.

Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.

Applications Reported: This UC10-4B9 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.

Applications Tested: This UC10-4B9 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellualr proteins located under the Resrouce Tab online. This can be used at less than or equal to 1 µg per million cells in a 100 µL total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

RANKL blockade improves efficacy of PD1-PD-L1 blockade or dual PD1-PD-L1 and CTLA4 blockade in mouse models of cancer.

Oncoimmunology

Ahern E,Harjunpää H,O'Donnell JS,Allen S,Dougall WC,Teng MWL,Smyth MJ

17-1522 was used in Flow cytometry/Cell sorting to set the scene for clinical evaluation of denosumab use in patients receiving contemporary immune checkpoint blockade.

Tue Jan 12 00:00:00 EST 2021

Activated autophagy restored the impaired frequency and function of regulatory T cells in chronic prostatitis.

The Prostate

Liu Y,Zhang Y,Zhang M,Meng J,Ma Q,Hao Z,Zheng M,Zhang L,Chen X,Liang C

Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 17-1522-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

MEF2D sustains activation of effector Foxp3+ Tregs during transplant survival and anticancer immunity.

The Journal of clinical investigation

Di Giorgio E,Wang L,Xiong Y,Akimova T,Christensen LM,Han R,Samanta A,Trevisanut M,Bhatti TR,Beier UH,Hancock WW

17-1522 was used in Flow cytometry/Cell sorting to study the role played by MEF2D in sustaining effector Foxp3+ Treg functions without abrogating their basal actions suggests its suitability for drug discovery efforts in cancer therapy.

Tue Dec 01 00:00:00 EST 2020

Mutation of an L-Type Calcium Channel Gene Leads to T Lymphocyte Dysfunction.

Frontiers in immunology

Fenninger F,Han J,Stanwood SR,Nohara LL,Arora H,Choi KB,Munro L,Pfeifer CG,Shanina I,Horwitz MS,Jefferies WA

Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 17-1522-82) in Flow Cytometry

Thu Oct 29 00:00:00 EDT 2020

In Situ Therapeutic Cancer Vaccination with an Oncolytic Virus Expressing Membrane-Tethered IL-2.

Molecular therapy oncolytics

Liu W,Dai E,Liu Z,Ma C,Guo ZS,Bartlett DL

17-1522 was used in Flow cytometry/Cell sorting to establish in situ anticancer immunotherapy regimens.

Fri Jun 26 00:00:00 EDT 2020

Pulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer.

Cell reports

Choi H,Deng J,Li S,Silk T,Dong L,Brea EJ,Houghton S,Redmond D,Zhong H,Boiarsky J,Akbay EA,Smith PD,Merghoub T,Wong KK,Wolchok JD

17-1522 was used in Flow cytometry/Cell sorting to set the foundation and show the importance of a combinatorial therapeutic strategy using pulsatile targeted therapy together with immunotherapy to optimally enhance tumor delay and promote long-term anti-tumor immunity.

Tue Apr 16 00:00:00 EDT 2019

Modifying the cancer-immune set point using vaccinia virus expressing re-designed interleukin-2.

Nature communications

Liu Z,Ge Y,Wang H,Ma C,Feist M,Ju S,Guo ZS,Bartlett DL

17-1522 was used in Flow cytometry/Cell sorting to explore a variety of IL-2 constructs expressed by a tumour-selective oncolytic vaccinia virus, designed to maintain IL-2 in the tumour microenvironment to reduce systemic toxicity.

Thu Nov 08 00:00:00 EST 2018

Cambogin suppresses dextran sulphate sodium-induced colitis by enhancing Treg cell stability and function.

British journal of pharmacology

Lu Y,Kim NM,Jiang YW,Zhang H,Zheng D,Zhu FX,Liang R,Li B,Xu HX

Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 17-1522-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Oncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer Vaccination.

Cancer research

Jiang H,Rivera-Molina Y,Gomez-Manzano C,Clise-Dwyer K,Bover L,Vence LM,Yuan Y,Lang FF,Toniatti C,Hossain MB,Fueyo J

17-1522 was used in Flow cytometry/Cell sorting to demonstrate that combining an oncolytic virus with tumor-targeting immune checkpoint modulators elicits potent in situ autologous cancer vaccination, resulting in an efficacious, tumor-specific, and long-lasting therapeutic effect.

Sat Jul 15 00:00:00 EDT 2017

Cblb-deficient T cells are less susceptible to PD-L1-mediated inhibition.

Oncotarget

Peer S,Baier G,Gruber T

17-1522 was used in Flow cytometry/Cell sorting to show that Cblb-/- T cells are less susceptible to PD-L1-mediated suppression of T cell proliferation and IFNγ secretion.

Tue Jun 27 00:00:00 EDT 2017

Stability and function of regulatory T cells expressing the transcription factor T-bet.

Nature

Levine AG,Mendoza A,Hemmers S,Moltedo B,Niec RE,Schizas M,Hoyos BE,Putintseva EV,Chaudhry A,Dikiy S,Fujisawa S,Chudakov DM,Treuting PM,Rudensky AY

17-1522 was used in Flow cytometry/Cell sorting to demonstrate that T-bet+ Treg cells have an essential immunosuppressive function.

Thu Jun 15 00:00:00 EDT 2017

CD117⁺CD44⁺ Stem T Cells Develop in the Thymus and Potently Suppress T-cell Proliferation by Modulating the CTLA-4 Pathway.

Stem cell research & therapy

Wei Y,Hu Z,Gu W,Liu G,Shi B,Liu E,Liu T

17-1522 was used in Flow cytometry/Cell sorting to suggest that CD44+CD117+ T cells are stem cells and a specific T-cell phenotype that initially develops in the thymus, but they do not progress through DN3 and DN4 stages, lack a DP stage, and potently suppress T-cell proliferation and modulate the CTLA-4 pathway.

Thu Mar 09 00:00:00 EST 2017

Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.

Nature communications

Zamarin D,Holmgaard RB,Ricca J,Plitt T,Palese P,Sharma P,Merghoub T,Wolchok JD,Allison JP

Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 17-1522-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2017

Glucocorticoid receptor in T cells mediates protection from autoimmunity in pregnancy.

Proceedings of the National Academy of Sciences of the United States of America

Engler JB,Kursawe N,Solano ME,Patas K,Wehrmann S,Heckmann N,Lühder F,Reichardt HM,Arck PC,Gold SM,Friese MA

17152282 was used in flow cytometry to characterize conventional and regulatory T cells during pregnancy

Tue Jan 10 00:00:00 EST 2017

Context-dependent regulation of Th17-associated genes and IFNγ expression by the transcription factor NFAT5.

Immunology and cell biology

Alberdi M,Iglesias M,Tejedor S,Merino R,López-Rodríguez C,Aramburu J

17-1522 was used in Flow cytometry/Cell sorting to show that NFAT5 can modulate different T-cell responses depending on stress conditions and stimulatory context.

Sun Jan 01 00:00:00 EST 2017

Interleukin-37 Enhances the Suppressive Activity of Naturally Occurring CD4⁺CD25⁺ Regulatory T Cells.

Scientific reports

Wang DW,Dong N,Wu Y,Zhu XM,Wang CT,Yao YM

17-1522-82 was used in Flow Cytometry to conclude that rhIL-37 enhances the suppressive activity of CD4+CD25+ Tregs and might be a potential immunomodulator for the treatment of septic complications.

Mon Dec 12 00:00:00 EST 2016

Interdependent IL-7 and IFN-γ signalling in T-cell controls tumour eradication by combined α-CTLA-4+α-PD-1 therapy.

Nature communications

Shi LZ,Fu T,Guan B,Chen J,Blando JM,Allison JP,Xiong L,Subudhi SK,Gao J,Sharma P

Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 17-1522-82) in Flow Cytometry

Mon Aug 08 00:00:00 EDT 2016

Favorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors.

PloS one

Tähtinen S,Kaikkonen S,Merisalo-Soikkeli M,Grönberg-Vähä-Koskela S,Kanerva A,Parviainen S,Vähä-Koskela M,Hemminki A

Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 17-1522-82) in Flow Cytometry

Thu Apr 28 00:00:00 EDT 2016

Programmed cell death receptor ligand 1 modulates the regulatory T cells' capacity to repress shock/sepsis-induced indirect acute lung injury by recruiting phosphatase SRC homology region 2 domain-containing phosphatase 1.

Shock (Augusta, Ga.)

Tang L,Bai J,Chung CS,Lomas-Neira J,Chen Y,Huang X,Ayala A

17-1522 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which the programmed cell death receptor 1 ligand suppresses lung injury.

Thu Jan 01 00:00:00 EST 2015

Active players in resolution of shock/sepsis induced indirect lung injury: immunomodulatory effects of Tregs and PD-1.

Journal of leukocyte biology

Tang L,Bai J,Chung CS,Lomas-Neira J,Chen Y,Huang X,Ayala A

17-1522 was used in Flow cytometry/Cell sorting to explore the ability of Tregs to modify the innate immune response induced by experimental iALI.

Sat Nov 01 00:00:00 EDT 2014

Paracaspase MALT1 deficiency protects mice from autoimmune-mediated demyelination.

Journal of immunology (Baltimore, Md. : 1950)

Mc Guire C,Wieghofer P,Elton L,Muylaert D,Prinz M,Beyaert R,van Loo G

17-1522 was used in Flow cytometry/Cell sorting to study the role of MALT1 in the development of experimental autoimmune encephalomyelitis.

Fri Mar 15 00:00:00 EDT 2013

Murine CD4⁺CD25⁻ cells activated in vitro with PMA/ionomycin and anti-CD3 acquire regulatory function and ameliorate experimental colitis in vivo.

BMC gastroenterology

Majowicz A,van der Marel S,te Velde AA,Meijer SL,Petry H,van Deventer SJ,Ferreira V

17-1522 was used in Flow cytometry/Cell sorting to identify a new method to generate in vitro iTreg cells (TregPMA cells) with demonstrated functionality in vivo.

Mon Dec 03 00:00:00 EST 2012

IL-10+ CTLA-4+ Th2 inhibitory cells form in a Foxp3-independent, IL-2-dependent manner from Th2 effectors during chronic inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Altin JA,Goodnow CC,Cook MC

17-1522 was used in Flow cytometry/Cell sorting to investigate the mechanism and molecules involved in the formation of IL10+CTLA-4+Th2 inhibitory cells during chronic inflammation.