Invitrogen

CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), APC, eBioscience™

Product Image Gallery

CD152 (CTLA-4) Antibody — Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 17-1522-82)

CD152 (CTLA-4) Antibody in Flow Cytometry (Flow) — C57Bl/6 splenocytes were unstimulated (left) or stimulated with Con A for 3 days (right). Cells were intracellularly stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0041-82) and with 0.5 µg of Armenian Hamster IgG Isotype Control APC (Product # 17-4888-82) (blue histogram) or 0.5 µg of Anti-Mouse CD152 (CTLA-4) APC (purple histogram) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the CD4+ gate were used for analysis.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1 µg/Test

Published Applications

Flow Cytometry (Flow)

See 14 publications below

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host / Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

UC10-4B9

Conjugate

APC

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2016700

Product Specific Information

Description: The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells at low level and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly.

Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.

Applications Reported: This UC10-4B9 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.

Applications Tested: This UC10-4B9 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellualr proteins located under the Resrouce Tab online. This can be used at less than or equal to 1 µg per million cells in a 100 µL total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD152; CD152 antigen; CD152 isoform; celiac disease 3; CELIAC3; CTLA-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; cytotoxic T-lymphocyte antigen 4; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; GRD4; ICOS; IDDM12

Gene Aliases: Cd152; Ctla-4; Ctla4; Ly-56

UniProt ID: (Mouse) P09793

Entrez Gene ID: (Mouse) 12477

Molecular Function: cytokine signaling molecule