Invitrogen

CD16 Recombinant Rabbit Monoclonal Antibody (JE49-79)

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CD16 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CD16 in paraffin-embedded human spleen tissue using a monoclonal antibody (Product #MA5-36143). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

CD16 Antibody in Western Blot (WB) — Western blot analysis of CD16 using a CD16 monoclonal antibody (Product #MA5-36143). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody at a dilution of 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

Product Details

MA5-36143

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JE49-79
Immunogen	Recombinant protein within Human CD16 aa 151-254 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target Information

CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms – FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FCGR3A is associated, with FcepsilonRI-beta subunit. Besides IgG, FCGR3A can be triggered also by oligomeric IgE. FCGR3B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a pro-adhesive phenotype. Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway.

Journal of neuroinflammation

Xu X,Gao W,Li L,Hao J,Yang B,Wang T,Li L,Bai X,Li F,Ren H,Zhang M,Zhang L,Wang J,Wang D,Zhang J,Jiao L

MA5-36143 was used in Western Blot to report for the first time that the decreased ANXA1 in patients with AIS were recovered 2nd–3rd after successful recanalization by EVT.

Sat May 22 00:00:00 EDT 2021

Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway.

Journal of neuroinflammation

Xu X,Gao W,Li L,Hao J,Yang B,Wang T,Li L,Bai X,Li F,Ren H,Zhang M,Zhang L,Wang J,Wang D,Zhang J,Jiao L

MA5-36143 was used in Western Blot to report for the first time that the decreased ANXA1 in patients with AIS were recovered 2nd–3rd after successful recanalization by EVT.

Sat May 22 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: CD16-2; CD16a; CD16a antigen; CD16b; CD32 receptor 2; Fc fragment of IgG intermediate affinity IV receptor; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III; Fc gamma receptor III-A; Fc gamma receptor IIIa; Fc gamma receptor IIIb; Fc gammaRIV; Fc receptor-like 3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; Fc-gamma RIIIa; fc-gamma RIIIb; Fcg receptor III; FCG2; FcgammaRIII; FcgammaRIIIA; FcgammaRIV; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; fcRIII; igG Fc receptor III; IgG Fc receptor III-1; IgG Fc receptor III-2; immunoglobulin G Fc receptor III; low affinity immunoglobulin gamma Fc region receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; Low affinity immunoglobulin gamma Fc region receptor IV; neutrophil-specific antigen NA; RP11-5K23.1; transmembrane receptor FcgammaRIII-X

Gene Aliases: 4833442P21Rik; CD16; CD16-2; CD16A; CD16B; FCG3; FcgammaRIV; FCGR3; FCGR3A; FCGR3B; Fcgr4; FCGRIII; FcgRIV; FCR-10; FCRIII; FCRIIIA; FCRIIIb; Fcrl3; IGFR3; IMD20

UniProt ID: (Human) P08637, (Human) O75015, (Mouse) A0A0B4J1G0

Entrez Gene ID: (Human) 2214, (Human) 2215, (Mouse) 14131, (Rat) 304966, (Mouse) 246256

Molecular Function: immunoglobulin receptor superfamily