|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||PU5 1.8 IOE7 Balb/c mouse cell line.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:25-1:50|
|Functional Assay (FN)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01EU/ug.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD16 is a 50-65kD cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. CD32 antigen is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Apoptosis of mouse mast cells is reciprocally regulated by the IgG receptors Fc¿RIIB and Fc¿RIIIA.
MA5-16680 was used in flow cytometry to assess if mast cells respond to apoptosis induction by IgG immune complex aggregation of Fcγ receptors.
|Fang Y,Larsson L,Bruhns P,Xiang Z||Allergy (67:1233)||2012|