Invitrogen

CD16/CD32 Monoclonal Antibody (93), APC, eBioscience™

Product Image Gallery

CD16/CD32 Antibody in Flow Cytometry (Flow) — Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 µg of Anti-Mouse CD16/CD32 APC (filled histogram). Total viable cells were used for analysis.

Product Details

17-0161-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.25 µg/test	View 18 publications 18 publications

Product Specifications
Species Reactivity	Mouse
Published species	Fruit fly, Mouse, Yeast
Host/Isotype	Rat / IgG2a, lambda
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	93
Conjugate	APC Alexa Fluor 700 Biotin Brilliant UV 496 Brilliant UV 661 eFluor 450 FITC Functional Grade NovaFluor Red 685 NovaFluor Red 700 NovaFluor Red 710 NovaFluor Red 755 NovaFluor Yellow 690 PE PE-Cyanine7 PerCP-Cyanine5.5 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Unconjugated
Excitation/Emission Max	651/660 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_469356

Product Specific Information

Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Cholesterol and sphingomyelin are critical for Fcγ receptor-mediated phagocytosis of Cryptococcus neoformans by macrophages.

The Journal of biological chemistry

Bryan AM,You JK,Li G,Kim J,Singh A,Morstein J,Trauner D,Pereira de Sá N,Normile TG,Farnoud AM,London E,Del Poeta M

17-0161-82 was used in Flow Cytometry to suggest that lipid rafts are critical for facilitating FcγRIII-mediated phagocytosis of C. neoformans.

Wed Dec 01 00:00:00 EST 2021

TNFAIP3 Plays a Role in Aging of the Hematopoietic System.

Frontiers in immunology

Smith MA,Culver-Cochran AE,Adelman ER,Rhyasen GW,Ma A,Figueroa ME,Starczynowski DT

17-0161-82 was used in Flow Cytometry to find that the expression of A20 is significantly reduced in aged hematopoietic stem and progenitor cells (HSPC) as compared to young HSPC.

Wed Apr 28 00:00:00 EDT 2021

miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

Nature communications

Lam J,van den Bosch M,Wegrzyn J,Parker J,Ibrahim R,Slowski K,Chang L,Martinez-Høyer S,Condorelli G,Boldin M,Deng Y,Umlandt P,Fuller M,Karsan A

Published figure using CD16/CD32 monoclonal antibody (Product # 17-0161-82) in Flow Cytometry

Wed Jun 20 00:00:00 EDT 2018

MiR-125a Is a critical modulator for neutrophil development.

PLoS genetics

Qin Y,Wu L,Ouyang Y,Zhou P,Zhou H,Wang Y,Ma J,Zhang J,Chen Y,Qian J,Tang Y,Shen N

17-0161 was used in Flow cytometry/Cell sorting to reveal a microRNA regulating granulocyte development and supporte a model in which miR-125a acts as a fine-tuner of granulopoiesis.

Sun Oct 01 00:00:00 EDT 2017

Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.

Nature

Hérault A,Binnewies M,Leong S,Calero-Nieto FJ,Zhang SY,Kang YA,Wang X,Pietras EM,Chu SH,Barry-Holson K,Armstrong S,Göttgens B,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 17-0161-82) in Flow Cytometry

Thu Apr 06 00:00:00 EDT 2017

Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.

Nature communications

Liu Z,Ravindranathan R,Kalinski P,Guo ZS,Bartlett DL

Published figure using CD16/CD32 monoclonal antibody (Product # 17-0161-82) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2017

Ubiquitination of hnRNPA1 by TRAF6 links chronic innate immune signaling with myelodysplasia.

Nature immunology

Fang J,Bolanos LC,Choi K,Liu X,Christie S,Akunuru S,Kumar R,Wang D,Chen X,Greis KD,Stoilov P,Filippi MD,Maciejewski JP,Garcia-Manero G,Weirauch MT,Salomonis N,Geiger H,Zheng Y,Starczynowski DT

17-0161 was used in Flow cytometry/Cell sorting to examine the significance of ubiquitin signalling in coordinating RNA processing by Toll-like receptor pathways during an immune response and in premalignant haematologic diseases.

Wed Feb 01 00:00:00 EST 2017

Agonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial glioma.

Journal for immunotherapy of cancer

Patel MA,Kim JE,Theodros D,Tam A,Velarde E,Kochel CM,Francica B,Nirschl TR,Ghasemzadeh A,Mathios D,Harris-Bookman S,Jackson CC,Jackson C,Ye X,Tran PT,Tyler B,Coric V,Selby M,Brem H,Drake CG,Pardoll DM,Lim M

17-0161 was used in Flow cytometry/Cell sorting to provide preclinical evidence for the use of anti-GITR (1) non-depleting antibodies in combination with SRS in GBM.

Wed May 18 00:00:00 EDT 2016

Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model.

Molecular medicine reports

Yue Y,Li P,Song N,Li B,Li Z,Guo Y,Zhang W,Wei MQ,Gai Z,Meng H,Wang J,Qin L

17-0161 was used in Flow cytometry/Cell sorting to understand the virological features of enterovirus 71 and the host responses resulting from enterovirus 71 infection.

Sun May 01 00:00:00 EDT 2016

Gliotoxin Suppresses Macrophage Immune Function by Subverting Phosphatidylinositol 3,4,5-Trisphosphate Homeostasis.

mBio

Schlam D,Canton J,Carreño M,Kopinski H,Freeman SA,Grinstein S,Fairn GD

Published figure using CD16/CD32 monoclonal antibody (Product # 17-0161-82) in Flow Cytometry

Tue Apr 05 00:00:00 EDT 2016

Functions of miR-146a and miR-222 in Tumor-associated Macrophages in Breast Cancer.

Scientific reports

Li Y,Zhao L,Shi B,Ma S,Xu Z,Ge Y,Liu Y,Zheng D,Shi J

17-0161 was used in Flow cytometry/Cell sorting to study the role of endogenous miRNAs in controlling the polarisation and function of tumour-associated macrophages in breast cancer.

Tue Dec 22 00:00:00 EST 2015

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using CD16/CD32 monoclonal antibody (Product # 17-0161-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

Elevating body temperature enhances hematopoiesis and neutrophil recovery after total body irradiation in an IL-1-, IL-17-, and G-CSF-dependent manner.

Blood

Capitano ML,Nemeth MJ,Mace TA,Salisbury-Ruf C,Segal BH,McCarthy PL,Repasky EA

17-0161 was used in Flow cytometry/Cell sorting to study the factors limiting the immune system recovery process after chemo- and radiotherapy.

Thu Sep 27 00:00:00 EDT 2012

Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms.

The American journal of pathology

Ninković J,Roy S

Published figure using CD16/CD32 monoclonal antibody (Product # 17-0161-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms.

The American journal of pathology

Ninković J,Roy S

Published figure using CD16/CD32 monoclonal antibody (Product # 17-0161-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

A high omega-3 fatty acid diet has different effects on early and late stage myeloid progenitors.

Lipids

Varney ME,Buchanan JT,Dementieva Y,Hardman WE,Sollars VE

17-0161 was used in Flow cytometry/Cell sorting to examine the effects of n-3 and n-6 fatty acids on haematopietic stem and progenitor cells in vivo in the mouse.

Sat Jan 01 00:00:00 EST 2011

Mice lacking Tbk1 activity exhibit immune cell infiltrates in multiple tissues and increased susceptibility to LPS-induced lethality.

Journal of leukocyte biology

Marchlik E,Thakker P,Carlson T,Jiang Z,Ryan M,Marusic S,Goutagny N,Kuang W,Askew GR,Roberts V,Benoit S,Zhou T,Ling V,Pfeifer R,Stedman N,Fitzgerald KA,Lin LL,Hall JP

17-0161 was used in Flow cytometry/Cell sorting to analyse the immune problems arising from production of a truncated Tbk1 protein in mice.

Wed Dec 01 00:00:00 EST 2010

Expression and role of FLT3 in regulation of the earliest stage of normal granulocyte-monocyte progenitor development.

Blood

Böiers C,Buza-Vidas N,Jensen CT,Pronk CJ,Kharazi S,Wittmann L,Sitnicka E,Hultquist A,Jacobsen SE

17-0161 was used in Flow cytometry/Cell sorting to investigate the role of FLT3 in early granulocyte-monocyte progenitors (GMPs), showing that most GMPs are derived from FLT3-expressing progenitors.

Thu Jun 17 00:00:00 EDT 2010

Immune depletion with cellular mobilization imparts immunoregulation and reverses autoimmune diabetes in nonobese diabetic mice.

Diabetes

Parker MJ,Xue S,Alexander JJ,Wasserfall CH,Campbell-Thompson ML,Battaglia M,Gregori S,Mathews CE,Song S,Troutt M,Eisenbeis S,Williams J,Schatz DA,Haller MJ,Atkinson MA

17-0161 was used in Flow cytometry/Cell sorting to investigate the use of ATG and GCSF in therapies to reverse overt hyperglycaemia in a diabetic mouse model.

Thu Oct 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD16A; CD16B; CD32; CD32 receptor 2; Fc gamma receptor IIB; Fc gamma receptor III; Fc gamma RIIB; Fc-gamma RII; fc-gamma RIII; Fc-gamma-RII; fc-gamma-RIIB; Fcg receptor III; FCG2; FcgammaRIIB; FcgammaRIII; FCGR2C; FCGR3A; FCGR3B; FCGRIII; FCR-10; FcRII-b; FcRII-c; fcRIII; FCRIIIA; FGFR2B; IgG Fc receptor II beta; igG Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; low affinity immunoglobulin gamma Fc region receptor III; Ly-17; Lymphocyte antigen 17; RP11-5K23.1

Gene Aliases: AI528646; CD16; CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; Fcgr2b; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1

UniProt ID: (Mouse) P08101

Entrez Gene ID: (Mouse) 14130, (Mouse) 14131

Molecular Function: immunoglobulin receptor superfamily

Function(s)

Process(es)

receptor signaling protein activity protein binding IgG binding immunoglobulin binding IgG receptor activity

CD16/CD32 Monoclonal Antibody (93), APC, eBioscience™

Product Details

17-0161-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

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