Invitrogen

CD16/CD32 Monoclonal Antibody (93), PerCP-eFluor™ 710, eBioscience™

Product Image Gallery

CD16/CD32 Antibody in Flow Cytometry (Flow) — Staining of C57BL/6 splenocytes with Anti-Mouse CD45RB APC (Product # 17-0455-81) and 0.125 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or 0.06 µg of Anti-Mouse CD16/CD32 PerCP-eFluor® 710 (right). Total viable cells were used for analysis.

Product Details

46-0161-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.125 µg/test	View 22 publications 22 publications

Product Specifications
Species Reactivity	Mouse
Published species	Fruit fly, Mouse
Host/Isotype	Rat / IgG2a, lambda
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	93
Conjugate	PerCP-eFluor™ 710 Alexa Fluor 700 APC Biotin eFluor 450 FITC Functional Grade NovaFluor Red 685 NovaFluor Red 700 NovaFluor Red 710 NovaFluor Red 755 NovaFluor Yellow 690 PE PE-Cyanine7 PerCP-Cyanine5.5 Super Bright 436 Super Bright 600 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2016701

Product Specific Information

Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported: This 93 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

46-0161-82 was used in Flow Cytometry to understand the effects of age-related systemic factors on hematopoietic stem cells and their bone marrow niche.

Mon Jul 05 00:00:00 EDT 2021

Snai2 Maintains Bone Marrow Niche Cells by Repressing Osteopontin Expression.

Developmental cell

Wei Q,Nakahara F,Asada N,Zhang D,Gao X,Xu C,Alfieri A,Brodin NP,Zimmerman SE,Mar JC,Guha C,Guo W,Frenette PS

46-0161-82 was used in Flow Cytometry to conclude that SNAI2 is a critical regulator of the transcriptional network maintaining MSPCs by the suppression of osteopontin expression.

Mon Jun 08 00:00:00 EDT 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

46-0161 was used in Flow cytometry/Cell sorting to uncover a mechanism that controls myeloid regeneration and early lineage decisions in HSCs and could be targeted in LSCs to normalize leukemic myeloid cell production.

Mon Mar 02 00:00:00 EST 2020

TNF-α Coordinates Hematopoietic Stem Cell Survival and Myeloid Regeneration.

Cell stem cell

Yamashita M,Passegué E

46-0161 was used in Flow cytometry/Cell sorting to reveal several TNF-α-mediated pro-survival mechanisms unique to HSCs, highlight an important role for necroptosis in HSC killing, and establish TNF-α as a major pro-survival and pro-regeneration factor for HSCs.

Thu Sep 05 00:00:00 EDT 2019

Bone marrow adipose tissue-derived stem cell factor mediates metabolic regulation of hematopoiesis.

Haematologica

Zhang Z,Huang Z,Ong B,Sahu C,Zeng H,Ruan HB

46-0161-82 was used in Flow Cytometry to demonstrate that marrow adipose tissue provides stem cell factor essentially for hematopoiesis both at the steady state and upon metabolic stresses.

Sun Sep 01 00:00:00 EDT 2019

Bone marrow adipose tissue-derived stem cell factor mediates metabolic regulation of hematopoiesis.

Haematologica

Zhang Z,Huang Z,Ong B,Sahu C,Zeng H,Ruan HB

46-0161-82 was used in Flow Cytometry to demonstrate that marrow adipose tissue provides stem cell factor essentially for hematopoiesis both at the steady state and upon metabolic stresses.

Sun Sep 01 00:00:00 EDT 2019

miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

Nature communications

Lam J,van den Bosch M,Wegrzyn J,Parker J,Ibrahim R,Slowski K,Chang L,Martinez-Høyer S,Condorelli G,Boldin M,Deng Y,Umlandt P,Fuller M,Karsan A

Published figure using CD16/CD32 monoclonal antibody (Product # 46-0161-82) in Flow Cytometry

Wed Jun 20 00:00:00 EDT 2018

Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.

Nature

Hérault A,Binnewies M,Leong S,Calero-Nieto FJ,Zhang SY,Kang YA,Wang X,Pietras EM,Chu SH,Barry-Holson K,Armstrong S,Göttgens B,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 46-0161-82) in Flow Cytometry

Thu Apr 06 00:00:00 EDT 2017

Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.

Nature communications

Liu Z,Ravindranathan R,Kalinski P,Guo ZS,Bartlett DL

Published figure using CD16/CD32 monoclonal antibody (Product # 46-0161-82) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2017

Human umbilical cord blood-borne fibroblasts contain marrow niche precursors that form a bone/marrow organoid in vivo.

Development (Cambridge, England)

Pievani A,Sacchetti B,Corsi A,Rambaldi B,Donsante S,Scagliotti V,Vergani P,Remoli C,Biondi A,Robey PG,Riminucci M,Serafini M

46-0161 was used in Flow cytometry/Cell sorting to show that human umbilical cord blood-borne fibroblasts are able to recapitulate the conditions by which the bone marrow microenvironment is formed and establish complete hematopoietic stem cell niches.

Wed Mar 15 00:00:00 EDT 2017

Autophagy maintains the metabolism and function of young and old stem cells.

Nature

Ho TT,Warr MR,Adelman ER,Lansinger OM,Flach J,Verovskaya EV,Figueroa ME,Passegué E

46-0161 was used in Flow cytometry/Cell sorting to demonstrate an essential function for autophagy in removing activated mitochondria and controlling oxidative metabolism, thereby maintaining HSC stemness and regenerative potential.

Thu Mar 09 00:00:00 EST 2017

Differential cytokine contributions of perivascular haematopoietic stem cell niches.

Nature cell biology

Asada N,Kunisaki Y,Pierce H,Wang Z,Fernandez NF,Birbrair A,Ma'ayan A,Frenette PS

46-0161 was used in Flow cytometry/Cell sorting to demonstrate that the effects of cytokines regulating haematopoietic stem cell functions are dependent on the producing cell sources.

Wed Mar 01 00:00:00 EST 2017

CD8⁺ T cells drive autoimmune hematopoietic stem cell dysfunction and bone marrow failure.

Journal of autoimmunity

Gravano DM,Al-Kuhlani M,Davini D,Sanders PD,Manilay JO,Hoyer KK

46-0161 was used in Flow cytometry/Cell sorting to investigate whether CD8+ T cells play a non-redundant role in driving bone marrow failure.

Thu Dec 01 00:00:00 EST 2016

Single-cell analysis of mixed-lineage states leading to a binary cell fate choice.

Nature

Olsson A,Venkatasubramanian M,Chaudhri VK,Aronow BJ,Salomonis N,Singh H,Grimes HL

46-0161 was used in Flow cytometry/Cell sorting to propose that mixed-lineage states are obligatory during cell-fate specification, manifest differing frequencies because of their dynamic instability and are dictated by counteracting gene-regulatory networks.

Thu Sep 29 00:00:00 EDT 2016

Chronic interleukin-1 exposure drives haematopoietic stem cells towards precocious myeloid differentiation at the expense of self-renewal.

Nature cell biology

Pietras EM,Mirantes-Barbeito C,Fong S,Loeffler D,Kovtonyuk LV,Zhang S,Lakshminarasimhan R,Chin CP,Techner JM,Will B,Nerlov C,Steidl U,Manz MG,Schroeder T,Passegué E

46-0161 was used in Flow cytometry/Cell sorting to identify a critical regulatory circuit that tailors HSC responses to acute needs, and is likely to underlie deregulated blood homeostasis in chronic inflammation conditions.

Wed Jun 01 00:00:00 EDT 2016

Agonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial glioma.

Journal for immunotherapy of cancer

Patel MA,Kim JE,Theodros D,Tam A,Velarde E,Kochel CM,Francica B,Nirschl TR,Ghasemzadeh A,Mathios D,Harris-Bookman S,Jackson CC,Jackson C,Ye X,Tran PT,Tyler B,Coric V,Selby M,Brem H,Drake CG,Pardoll DM,Lim M

Published figure using CD16/CD32 monoclonal antibody (Product # 46-0161-82) in Flow Cytometry

Wed May 18 00:00:00 EDT 2016

DNMT3A Haploinsufficiency Transforms FLT3ITD Myeloproliferative Disease into a Rapid, Spontaneous, and Fully Penetrant Acute Myeloid Leukemia.

Cancer discovery

Meyer SE,Qin T,Muench DE,Masuda K,Venkatasubramanian M,Orr E,Suarez L,Gore SD,Delwel R,Paietta E,Tallman MS,Fernandez H,Melnick A,Le Beau MM,Kogan S,Salomonis N,Figueroa ME,Grimes HL

46-0161 was used in Flow cytometry/Cell sorting to study the mechanism by which Dnmt3a haploinsufficiency transforms Flt3-internal tandem duplication myeloproliferative disease within a clonogenic AML subpopulation.

Sun May 01 00:00:00 EDT 2016

Gliotoxin Suppresses Macrophage Immune Function by Subverting Phosphatidylinositol 3,4,5-Trisphosphate Homeostasis.

mBio

Schlam D,Canton J,Carreño M,Kopinski H,Freeman SA,Grinstein S,Fairn GD

Published figure using CD16/CD32 monoclonal antibody (Product # 46-0161-82) in Flow Cytometry

Tue Apr 05 00:00:00 EDT 2016

Replication stress caused by low MCM expression limits fetal erythropoiesis and hematopoietic stem cell functionality.

Nature communications

Alvarez S,Díaz M,Flach J,Rodriguez-Acebes S,López-Contreras AJ,Martínez D,Cañamero M,Fernández-Capetillo O,Isern J,Passegué E,Méndez J

46-0161 was used in Flow cytometry/Cell sorting to determine that haematopoietic progenitors are particularly sensitive to replication stress.

Mon Oct 12 00:00:00 EDT 2015

Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells.

Nature

Flach J,Bakker ST,Mohrin M,Conroy PC,Pietras EM,Reynaud D,Alvarez S,Diolaiti ME,Ugarte F,Forsberg EC,Le Beau MM,Stohr BA,Méndez J,Morrison CG,Passegué E

46-0161 was used in Flow cytometry/Cell sorting to study functional decline in ageing haematopoietic stem cells due to replicative stress.

Thu Aug 14 00:00:00 EDT 2014

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using CD16/CD32 monoclonal antibody (Product # 46-0161-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

FOXO3A directs a protective autophagy program in haematopoietic stem cells.

Nature

Warr MR,Binnewies M,Flach J,Reynaud D,Garg T,Malhotra R,Debnath J,Passegué E

46-0161 was used in Flow cytometry/Cell sorting to show how FOXO3A directs a protective autophagy program in haematopoietic stem cells.

Thu Feb 21 00:00:00 EST 2013

Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms.

The American journal of pathology

Ninković J,Roy S

Published figure using CD16/CD32 monoclonal antibody (Product # 46-0161-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms.

The American journal of pathology

Ninković J,Roy S

Published figure using CD16/CD32 monoclonal antibody (Product # 46-0161-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: CD16A; CD16B; CD32; CD32 receptor 2; Fc gamma receptor IIB; Fc gamma receptor III; Fc gamma RIIB; Fc-gamma RII; fc-gamma RIII; Fc-gamma-RII; fc-gamma-RIIB; Fcg receptor III; FCG2; FcgammaRIIB; FcgammaRIII; FCGR2C; FCGR3A; FCGR3B; FCGRIII; FCR-10; FcRII-b; FcRII-c; fcRIII; FCRIIIA; FGFR2B; IgG Fc receptor II beta; igG Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; low affinity immunoglobulin gamma Fc region receptor III; Ly-17; Lymphocyte antigen 17; RP11-5K23.1

Gene Aliases: AI528646; CD16; CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; Fcgr2b; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1

UniProt ID: (Mouse) P08101

Entrez Gene ID: (Mouse) 14130, (Mouse) 14131

Molecular Function: immunoglobulin receptor superfamily