Description: CD160 is a glycosylphosphatidylinositol (GPI)-anchored Ig-like glycoprotein first identified on human lymphocytes with the monoclonal antibody BY55. In mice, CD160 is expressed on almost all (intestinal intraepithelial lymphocytes) iIELs, NKT cells, most TCRgammadelta T cells, few NK cells and a minor subset of CD8+ T cells. Murine CD160 has been shown to bind to a wide range of classical and non classical MHC class I molecules and regulate NK cell activation. In vitro, CD3 activation of murine CD8+ T cells increases the expression of CD160 and induces the release of soluble CD160 (sCD160). In human, CD160 mAb cross-linking triggers TNF alpha, IFN gamma and IL-6 cytokine production by peripheral blood NK cells and inhibits tube formation and induces apoptosis of endothelial cells. In mice, cross-linking of CD160 with the CNX46-3 antibody regulates NK cell activation both positively and negatively, depending on the stimulus.
Applications Reported: This eBioCNX46-3 (CNX46-3) antibody has been reported for use in flow cytometric analysis, functional assays, immunoprecipitation, and immunoblotting (WB).
Applications Tested: This eBioCNX46-3 (CNX46-3) antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
CD160 is a receptor showing broad specificity for both classical and non-classical MHC class I molecules. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. It is involved in cell proliferation, cell surface receptor signaling pathways, cellular defense response, and regulation of immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD160; CD160 antigen; CD160 antigen, soluble form; natural killer cell BY55; natural killer cell receptor; Natural killer cell receptor BY55
Gene Aliases: AU045688; By55; Cd160
UniProt ID: (Mouse) O88875
Entrez Gene ID: (Mouse) 54215