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Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Flow Cytometry (Flow) |
0.125 µg/test | - |
Product Specifications | |
---|---|
Species Reactivity |
Rat |
Host/Isotype |
Mouse / IgG1, kappa |
Recommended Isotype Control |
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™ |
Class |
Monoclonal |
Type |
Antibody |
Clone |
10/78 |
Conjugate |
PerCP-eFluor™ 710 |
Excitation/Emission Max |
482/708 nm View spectra |
Form |
Liquid |
Concentration |
0.2 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_11218902 |
Description: This 10/78 monoclonal antibody recognizes rat CD161a, which is also known as NKR-P1A. A member of the NKR-P1 family of type II transmembrane C-type lectins, CD161a exists as a homodimer on all NK cells, some T cells, and monocytes. In the rat, this activating receptor is also detectable on B220+ single positive B cells. CD161a is involved in activating NK cell-mediated cytotoxicity via phosphoinositide turnover and intracellular calcium mobilization.
The 10/78 monoclonal antibody has been reported to recognize both NKR-P1A and NKR-P1B.
Applications Reported: This 10/78 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 10/78 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
KLRB1 (Killer cell lectin like receptor subfamily B, member 1, CD-161) protein is classified as a type II membrane protein because it has an external C terminus. KLRB1 cell surface antigen is expressed by almost all NK cells and in a small subset of CD3+ve T cells. KLRB1 is a homodimeric cell surface protein, comprising two chains with molecular weights ranging from 40-44kDa. KLRB1 plays an inhibitory role on natural killer (NK) cells. Activation of KLRB1 leads to acid sphingomyelnase/SMPD1 stimulation and an increase in intracellular ceramide. Moreover, there is also an activation of AKT1/PKB and RPS6KA1/RSK1 kinase stimulation, and T cell proliferation by anti-CD3. KLRB1 acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope and to the N-acetyllactosamine epitope. KLRB1 also binds to CLEC2D/LLT1 as a ligand, and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Protein Aliases: Antigen 3.2.3; CD161 antigen-like family member A; CD161 antigen-like family member B; CD161a; CD161b; Immunoreceptor NKR-P1E; Killer cell lectin-like receptor subfamily B member 1; Killer cell lectin-like receptor subfamily B member 1A; killer cell lectin-like receptor subfamily B member 1B; Killer cell lectin-like receptor subfamily B member 1B allele A; killer cell lectin-like receptor subfamily B member 1B allele C; killer cell lectin-like receptor subfamily B member 1F; Killer cell lectin-like receptor subfamily B member 1G; klrb1b {ECO:0000312|EMBL:AAQ11375.1}; klrb1b {ECO:0000312|RGD:2975}; MGC138614; natural killer cell receptor; Natural killer cell surface protein NKR-P1B allele RNK/SD/BN/F344; natural killer cell surface protein NKR-P1B allele TO; Natural killer cell surface protein NKR-P1G; Natural killer cell surface protein P1-3.2.3; Natural killer lectin-like receptor 1E; natural killer lectin-like receptor protein 1 e; NKR-P1 3.2.3; NKR-P1A
Gene Aliases: Klrb1; Klrb1a; Klrb1b; Klrb1d; Klrb1f; Klrb1g; Klrb6; NKR-P1A; Nkr-p1b; Nkr-p1e; NKR-P1G; Nkrp-1e; Nkrp1a; Nkrp1b; Nkrp1e; Nkrp1g
UniProt ID: (Rat) Q0ZUP0, (Rat) P27471, (Rat) A4KWA1
Entrez Gene ID: (Rat) 689817, (Rat) 362443, (Rat) 25192
Molecular Function:
transmembrane signal receptor
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