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Invitrogen
CD161 Monoclonal Antibody (10/78), PerCP-eFluor™ 710, eBioscience™
FIGURE: 1 / 1
CD161 Antibody (46-1610-82) in Flow
Staining of rat splenocytes with 0.06 µg of Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or 0.06 µg of Anti-Rat CD161 PerCP-eFluor® 710 (purple histogram). Cells in the lymphocyte gate were used for analysis.
Product Details
46-1610-82
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
10/78
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11218902
Product Specific Information
Description: This 10/78 monoclonal antibody recognizes rat CD161a, which is also known as NKR-P1A. A member of the NKR-P1 family of type II transmembrane C-type lectins, CD161a exists as a homodimer on all NK cells, some T cells, and monocytes. In the rat, this activating receptor is also detectable on B220+ single positive B cells. CD161a is involved in activating NK cell-mediated cytotoxicity via phosphoinositide turnover and intracellular calcium mobilization.
The 10/78 monoclonal antibody has been reported to recognize both NKR-P1A and NKR-P1B.
Applications Reported: This 10/78 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 10/78 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
KLRB1 (Killer cell lectin like receptor subfamily B, member 1, CD-161) protein is classified as a type II membrane protein because it has an external C terminus. KLRB1 cell surface antigen is expressed by almost all NK cells and in a small subset of CD3+ve T cells. KLRB1 is a homodimeric cell surface protein, comprising two chains with molecular weights ranging from 40-44kDa. KLRB1 plays an inhibitory role on natural killer (NK) cells. Activation of KLRB1 leads to acid sphingomyelnase/SMPD1 stimulation and an increase in intracellular ceramide. Moreover, there is also an activation of AKT1/PKB and RPS6KA1/RSK1 kinase stimulation, and T cell proliferation by anti-CD3. KLRB1 acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope and to the N-acetyllactosamine epitope. KLRB1 also binds to CLEC2D/LLT1 as a ligand, and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antigen 3.2.3; CD161 antigen-like family member A; CD161 antigen-like family member B; CD161a; CD161b; Immunoreceptor NKR-P1E; Killer cell lectin-like receptor subfamily B member 1; Killer cell lectin-like receptor subfamily B member 1A; killer cell lectin-like receptor subfamily B member 1B; Killer cell lectin-like receptor subfamily B member 1B allele A; killer cell lectin-like receptor subfamily B member 1B allele C; killer cell lectin-like receptor subfamily B member 1F; Killer cell lectin-like receptor subfamily B member 1G; klrb1b {ECO:0000312|EMBL:AAQ11375.1}; klrb1b {ECO:0000312|RGD:2975}; MGC138614; natural killer cell receptor; Natural killer cell surface protein NKR-P1B allele RNK/SD/BN/F344; natural killer cell surface protein NKR-P1B allele TO; Natural killer cell surface protein NKR-P1G; Natural killer cell surface protein P1-3.2.3; Natural killer lectin-like receptor 1E; natural killer lectin-like receptor protein 1 e; NKR-P1 3.2.3; NKR-P1A
Gene Aliases: Klrb1; Klrb1a; Klrb1b; Klrb1d; Klrb1f; Klrb1g; Klrb6; NKR-P1A; Nkr-p1b; Nkr-p1e; NKR-P1G; Nkrp-1e; Nkrp1a; Nkrp1b; Nkrp1e; Nkrp1g
UniProt ID: (Rat) Q0ZUP0, (Rat) P27471, (Rat) A4KWA1
Entrez Gene ID: (Rat) 689817, (Rat) 362443, (Rat) 25192
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