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|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rat Spleen cell homogenate|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. A suggested positive control for immunohistochemical applications is liver. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01EU/ug.
CD163 antigen is a type I membrane protein also known as M130 antigen, Ber-Mac3, Ki-M8 or SM4. CD163 is restricted in its expression to the monocytic/macrophage lineage. It is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Eradication of rat malignant gliomas by retroviral-mediated, in vivo delivery of the interleukin 4 gene.
MA5-16655 was used in immunohistochemistry to test if IL-4 can protect from malignant gliomas in vivo.
|Benedetti S,Bruzzone MG,Pollo B,DiMeco F,Magrassi L,Pirola B,Cirenei N,Colombo MP,Finocchiaro G||Cancer research (59:645)||1999|
Limited efficacy of the HSV-TK/GCV system for gene therapy of malignant gliomas and perspectives for the combined transduction of the interleukin-4 gene.
MA5-16655 was used in immunohistochemistry to study the effects of IL-4 using the HSV-tk/GCV system of glioma gene therapy.
|Benedetti S,Dimeco F,Pollo B,Cirenei N,Colombo BM,Bruzzone MG,Cattaneo E,Vescovi A,Didonato S,Colombo MP,Finocchiaro G||Human gene therapy (8:1345)||1997|
CD163 antigen; hemoglobin scavenger receptor; macrophage-associated antigen; scavenger receptor cysteine-rich type 1 protein M130