Invitrogen

CD171 Monoclonal Antibody (UJ127), Biotin

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

11 References

View all (71) CD171 (L1CAM) antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

CD171 Antibody in Western Blot (WB) — Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1), PANC1 (Lane 2) and SKOV3 (Lane 3). The blots were probed with Mouse Anti-CD171 Monoclonal Antibody, Biotin (Product # MA5-14137, 1 µg/mL) and detected by chemiluminescence using Poly-HRP Streptavidin (Product # N200, 1:10,000 dilution). A 200 kDa corresponding to CD171 major form along with lower molecular mass form of 80 kDa corresponding to intracellular region of protein was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with wet transfer system. The membrane was probed with the relevant primary antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

CD171 Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human schwannoma stained with L1 Cell Adhesion Molecule antibody using peroxidase-conjugate and AEC chromogen. Note membrane staining of tumor cells.

CD171 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of L1 Cell Adhesion Molecule using L1 Cell Adhesion Molecule Monoclonal Antibody (Product # MA5-14137) on Native Human IMR-5 Cells.

CD171 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in CD171 KO cell line compared to wild type control cell line using CD171 Monoclonal Antibody (Product # MA5-14137). {KO}

Product Details

MA5-14137

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100	-
Flow Cytometry (Flow)	-	View 2 publications 2 publications
ELISA (ELISA)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	2 µg/mL	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	UJ127
Immunogen	Homogenous suspension of 16 week human fetal brain
Conjugate	Biotin Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10990347

Product Specific Information

MA5-14137 targets L1 Cell Adhesion Molecule in IHC (P), IP, and WB applications and shows reactivity with Human samples.

The MA5-14137 immunogen is homogenous suspension of 16 week human fetal brain.

Target Information

L1CAM/CD171 is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting L1 cell adhesion molecule expression using liposome-encapsulated siRNA suppresses prostate cancer bone metastasis and growth.

Oncotarget

Sung SY,Wu IH,Chuang PH,Petros JA,Wu HC,Zeng HJ,Huang WC,Chung LW,Hsieh CL

MA5-14137 was used in ELISA to determine how using liposome-encapsulated siRNA to target L1 cell adhesion molecule expression suppresses prostate cancer bone metastasis and growth

Thu Oct 30 00:00:00 EDT 2014

Adhesion molecule L1 is down-regulated in malignant peripheral nerve sheath tumors versus benign neurofibromatosis type 1-associated tumors.

Oral surgery, oral medicine, oral pathology and oral radiology

Blessmann M,Gröbe A,Quaas A,Kaifi JT,Mistakidis G,Bernreuther C,Sauter G,Gros S,Rawnaq T,Friedrich R,Mautner VF,Smeets R,Heiland M,Schachner M,Izbicki JR

MA5-14137 was used in immunohistochemistry to study the expression of the L1 adhesion molecule in malignant peripheral nerve sheath tumors and benign neurofibromatosis type 1-associated tumors

Wed Feb 01 00:00:00 EST 2012

Two alcohol binding residues interact across a domain interface of the L1 neural cell adhesion molecule and regulate cell adhesion.

The Journal of biological chemistry

Dou X,Menkari CE,Shanmugasundararaj S,Miller KW,Charness ME

MA5-14137 was used in flow cytometry to characterize two alcohol binding residues in L1 neural cell adhesion molecule and their involvement in cell adhesion

Fri May 06 00:00:00 EDT 2011

DOG1 and CD117 are the antibodies of choice in the diagnosis of gastrointestinal stromal tumours.

Histopathology

Novelli M,Rossi S,Rodriguez-Justo M,Taniere P,Seddon B,Toffolatti L,Sartor C,Hogendoorn PC,Sciot R,Van Glabbeke M,Verweij J,Blay JY,Hohenberger P,Flanagan A,Dei Tos AP

MA5-14137 was used in immunohistochemistry to study the value of COG1 and CD117 immunohistochemistry in the diagnosis of gastrointestinal stromal tumors

Sun Aug 01 00:00:00 EDT 2010

Subset of esophageal adenocarcinoma expresses adhesion molecule l1 in contrast to squamous cell carcinoma.

Anticancer research

Rawnaq T,Kleinhans H,Uto M,Schurr PG,Reichelt U,Cataldegirmen G,Gawad KA,Yekebas EF,Schachner M,Izbicki JR,Kaifi JT

MA5-14137 was used in immunohistochemistry to study neural cell adhesion molecule L1 overexpression in a subgroup of esophageal cancer

Wed Apr 01 00:00:00 EDT 2009

L1 is associated with favorable outcome in neuroblastomas in contrast to adult tumors.

Annals of surgical oncology

Wachowiak R,Fiegel HC,Kaifi JT,Quaas A,Krickhahn A,Schurr PG,Erttmann R,Schachner M,Kluth D,Sauter G,Izbicki JR

MA5-14137 was used in immunohistochemistry to study the association of the L1 neuronal adhesion molecule with favorable outcome in childhood neuroblastomas

Sat Dec 01 00:00:00 EST 2007

L1 is associated with micrometastatic spread and poor outcome in colorectal cancer.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Kaifi JT,Reichelt U,Quaas A,Schurr PG,Wachowiak R,Yekebas EF,Strate T,Schneider C,Pantel K,Schachner M,Sauter G,Izbicki JR

MA5-14137 was used in immunohistochemistry to study the role of the L1 adhesion molecule in micrometastatic spread and poor outcome in colorectal cancer

Thu Nov 01 00:00:00 EDT 2007

L1 (CD171) is highly expressed in gastrointestinal stromal tumors.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Kaifi JT,Strelow A,Schurr PG,Reichelt U,Yekebas EF,Wachowiak R,Quaas A,Strate T,Schaefer H,Sauter G,Schachner M,Izbicki JR

MA5-14137 was used in immunohistochemistry to study the expression of L1 (CD171) in gastrointestinal stromal tumors

Wed Mar 01 00:00:00 EST 2006

Characterization of gene expression in mucinous cystic neoplasms of the pancreas using oligonucleotide microarrays.

Oncogene

Fukushima N,Sato N,Prasad N,Leach SD,Hruban RH,Goggins M

MA5-14137 was used in immunohistochemistry to investigate the changes of gene expression in pancreatic mucinous cystic neoplasms

Thu Dec 02 00:00:00 EST 2004

Identification of cell surface and secreted proteins essential for tumor cell survival using a genetic suppressor element screen.

Oncogene

Gelman MS,Ye XK,Stull R,Suhy D,Jin L,Ng D,Than B,Ji M,Pan A,Perez P,Sun Y,Yeung P,Garcia LM,Harte R,Lu Y,Lamar E,Tavassoli R,Kennedy S,Osborn S,Chin DJ,Meshaw K,Holzmayer TA,Axenovich SA,Abo A

MA5-14137 was used in flow cytometry to identify molecular markers and therapeutic targets for tumors

Thu Oct 21 00:00:00 EDT 2004

Patterning axonal guidance molecules using a novel strategy for microcontact printing.

Neurochemical research

Oliva AA,James CD,Kingman CE,Craighead HG,Banker GA

MA5-14137 was used in western blot to develop a protocol for microprinting proteins on a substrate in order to control neurite growth in culture

Sat Nov 01 00:00:00 EST 2003

Bioinformatics

Protein Aliases: antigen identified by monoclonal antibody R1; CD 171; CD171; CD171 molecule; N-CAM-L1; Neural cell adhesion molecule L1; sCD171; sL1 CAM; sL1CAM; soluble CD 171; soluble CD171; soluble L1 CAM; soluble L1CAM

Gene Aliases: CAML1; CD171; HSAS; HSAS1; L1CAM; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1

UniProt ID: (Human) P32004

Entrez Gene ID: (Human) 3897

Function(s)

chemotaxis cell adhesion nervous system development axon guidance positive regulation of axon extension leukocyte migration