Invitrogen

CD171 Monoclonal Antibody (UJ127), Biotin

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View Details

11 References

View all (68) CD171 (L1CAM) antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

CD171 Antibody in Western Blot (WB) — Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1), PANC1 (Lane 2) and SKOV3 (Lane 3). The blots were probed with Mouse Anti-CD171 Monoclonal Antibody, Biotin (Product # MA5-14137, 1 µg/mL) and detected by chemiluminescence using Poly-HRP Streptavidin (Product # N200, 1:10,000 dilution). A 200 kDa corresponding to CD171 major form along with lower molecular mass form of 80 kDa corresponding to intracellular region of protein was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with wet transfer system. The membrane was probed with the relevant primary antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

CD171 Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human schwannoma stained with L1 Cell Adhesion Molecule antibody using peroxidase-conjugate and AEC chromogen. Note membrane staining of tumor cells.

CD171 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of L1 Cell Adhesion Molecule using L1 Cell Adhesion Molecule Monoclonal Antibody (Product # MA5-14137) on Native Human IMR-5 Cells.

CD171 Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of CD171 was observed in in SW480, PANC-1 and U-2 OS in comparison to Caco-2 using Anti-CD171 Monoclonal Antibody (UJ127), Biotin (Product # MA5-14137) in Western Blot. {RE}

CD171 Antibody in Knockout — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in CD171 KO cell line compared to wild type control cell line using CD171 Monoclonal Antibody (Product # MA5-14137). {KO}

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunoprecipitation (IP)

2 µg/mL

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 7 publications 7 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host / Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

UJ127

Immunogen

Homogenous suspension of 16 week human fetal brain

Conjugate

Biotin

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10990347

Product Specific Information

MA5-14137 targets L1 Cell Adhesion Molecule in IHC (P), IP, and WB applications and shows reactivity with Human samples.

The MA5-14137 immunogen is homogenous suspension of 16 week human fetal brain.

Target Information

L1CAM/CD171 is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: antigen identified by monoclonal antibody R1; CD 171; CD171; CD171 molecule; N-CAM-L1; Neural cell adhesion molecule L1; sCD171; sL1 CAM; sL1CAM; soluble CD 171; soluble CD171; soluble L1 CAM; soluble L1CAM

Gene Aliases: CAML1; CD171; HSAS; HSAS1; L1CAM; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1

UniProt ID: (Human) P32004

Entrez Gene ID: (Human) 3897

Molecular Function: G-protein coupled receptor cell adhesion molecule cytokine receptor defense/immunity protein hydrolase immunoglobulin receptor superfamily immunoglobulin superfamily cell adhesion molecule phosphatase protein phosphatase receptor