Invitrogen

CD176 Monoclonal Antibody (A78-G/A7)

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CD176 Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with Thomsen-Friedenreich Antigen antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of tumor cells.

CD176 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of Thomsen Friedenreich Antigen was performed using 70% confluent log phase HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Thomsen-Friedenreich Antigen (A78-G/A7) Mouse Monoclonal Antibody (Product # MA5-13466) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L)/IgM (L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:50

Immunocytochemistry (ICC)

2 µg/mL

Immunofluorescence (IF)

2 µg/mL

Product Specifications

Species Reactivity

Human, Rat

Published species

Pig

Host/Isotype

Mouse / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

A78-G/A7

Immunogen

Neuraminidase-treated human red blood cells

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10986703

Product Specific Information

MA5-13466 targets Thomsen-Friedenreich Antigen in immunofluorescence and immohistochemistry (Paraffin) applications and shows reactivity with Human and Rat samples. Staining of formalin-fixed tissues requires boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

The MA5-13466 immunogen is neuraminidase-treated human red blood cells.

Target Information

The Thomsen-Fridenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.