Flow cytometry analysis of CD19 was performed in mouse splenocytes. Mouse splenocytes were blocked using PBS containing 2.5% BSA for 45 minutes and were labeled with CD19 Rat Monoclonal Antibody (Product # MA1-10128) or with rat isotype control at 3-5µg/million cells in PBS. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rat Secondary Antibody (Product # A11006) at a dilution of 1:400 for 45 minutes at room temperature. Cells were acquired using an Attune® Acoustic Focusing Cytometer. Panel a and b represents cells labeled with the isotype control and CD19 Rat Monoclonal Antibody respectively.
|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Mouse CD19-transfected cell line|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 5 publications below|
CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Loss of Nrf2 exacerbates the visual deficits and optic neuritis elicited by experimental autoimmune encephalomyelitis.
MA1-10128 was used in flow cytometry to study the role of oxidative stress in optic neuritis
|Larabee CM,Desai S,Agasing A,Georgescu C,Wren JD,Axtell RC,Plafker SM||Molecular vision (22:1503)||2017|
NLRC5 shields T lymphocytes from NK-cell-mediated elimination under inflammatory conditions.
MA1-10128 was used in flow cytometry to assess the role of NLRC5 to NK-T-cell crosstalk
|Ludigs K,Jandus C,Utzschneider DT,Staehli F,Bessoles S,Dang AT,Rota G,Castro W,Zehn D,Vivier E,Held W,Romero P,Guarda G||Nature communications (7:null)||2016|
Treg cells require the phosphatase PTEN to restrain TH1 and TFH cell responses.
MA1-10128 was used in flow cytometry to show that the PTEN-mTORC2 axis maintains T regulatory cell stability and coordinates their control of effector responses
|Shrestha S,Yang K,Guy C,Vogel P,Neale G,Chi H||Nature immunology (16:178)||2015|
Hematopoietic progenitor cell lines with myeloid and lymphoid potential.
MA1-10128 was used in flow cytometry to generate and characterize Hoxb8-FL cells
|Redecke V,Wu R,Zhou J,Finkelstein D,Chaturvedi V,High AA,Häcker H||Nature methods (10:795)||2013|
The receptor tyrosine kinase Flt3 is required for dendritic cell development in peripheral lymphoid tissues.
MA1-10128 was used in flow cytometry to assess the effects of Flt3 signaling on macrophage dendritic cell progenitors and on peripheral dendritic cells
|Waskow C,Liu K,Darrasse-Jèze G,Guermonprez P,Ginhoux F,Merad M,Shengelia T,Yao K,Nussenzweig M||Nature immunology (9:676)||2008|