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Samples were acquired and analyzed using 405 nm excitation and specified band pass emission filter on a BD LSR II flow cytometer (BD Biosciences, San Jose, CA). The black line histogram represents cells stained with anti-human CD19 antibody conjugate, and the gray line represents unstained cells.
|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||mouse CD19 antigen|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation. It is expressed in all B cell precursor leukemias.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AW495831; B-lymphocyte antigen CD19; differentiation antigen CD19