|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Nalm 1 human cell line.|
|Storage buffer||PBS with proprietary stabilizer|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||10 µL/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation. It is expressed in all B cell precursor leukemias.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.
A15429 was used in flow cytometry to developed a strategy to expand umbilical cord blood T cells and test their effects after transplantation
|Pegram HJ,Purdon TJ,van Leeuwen DG,Curran KJ,Giralt SA,Barker JN,Brentjens RJ||Leukemia (29:415)||2015|
Capsid-specific T-cell responses to natural infections with adeno-associated viruses in humans differ from those of nonhuman primates.
A15429 was used in flow cytometry to test if rejection of AAV-transduced hepatocytes is due to AAV capsid-specific CD8 positive T cells that become reactivated upon gene transfer.
|Li H,Lasaro MO,Jia B,Lin SW,Haut LH,High KA,Ertl HC||Molecular therapy : the journal of the American Society of Gene Therapy (19:2021)||2011|
Effects of immunosuppression on circulating adeno-associated virus capsid-specific T cells in humans.
A15429 was used in immunocytochemistry to assess the effect of immunosuppression used for organ transplantation on immune responses to adeno-associated virus capsid antigens.
|Parzych EM,Li H,Yin X,Liu Q,Wu TL,Podsakoff GM,High KA,Levine MH,Ertl HC||Human gene therapy (24:431)||2013|