Invitrogen

CD19 Monoclonal Antibody (eBio1D3 (1D3)), PE-Cyanine5, eBioscience™

Product Image Gallery

CD19 Antibody in Flow Cytometry (Flow) — Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) (Product # 11-0452-82) and 0.125 µg of Rat IgG2a Isotype Control PE-Cyanine5 (Product # 15-4321-80) (left) or 0.125 µg of Anti-Mouse CD19 PE-Cyanine5 (right).Cells in the lymphocyte gate were used for analysis.

Product Details

15-0193-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.25 µg/test	View 58 publications 58 publications

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	eBio1D3 (1D3)
Conjugate	PE-Cyanine5 Alexa Fluor 561 Alexa Fluor 660 Alexa Fluor 700 APC APC-eFluor 780 Biotin Brilliant UV 395 Brilliant UV 496 Brilliant UV 563 Brilliant UV 615 Brilliant UV 661 Brilliant UV 737 Brilliant UV 805 Brilliant Violet 421 Brilliant Violet 480 Brilliant Violet 650 Brilliant Violet 786 eFluor 450 eFluor 506 eFluor 660 FITC Functional Grade NovaFluor Blue 510 NovaFluor Blue 530 NovaFluor Blue 5
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_657672

Product Specific Information

Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.

Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

Cellular and molecular gastroenterology and hepatology

Vonderhaar EP,Barnekow NS,McAllister D,McOlash L,Eid MA,Riese MJ,Tarakanova VL,Johnson BD,Dwinell MB

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

The Role of Allograft Inflammatory Factor-1 in the Effects of Experimental Diabetes on B Cell Functions in the Heart.

Frontiers in cardiovascular medicine

Sarkar A,Shukla SK,Alqatawni A,Kumar A,Addya S,Tsygankov AY,Rafiq K

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Mon Dec 06 00:00:00 EST 2021

Regulatory B Cells (Bregs) Inhibit Osteoclastogenesis and Play a Potential Role in Ameliorating Ovariectomy-Induced Bone Loss.

Frontiers in immunology

Sapra L,Bhardwaj A,Mishra PK,Garg B,Verma B,Mishra GC,Srivastava RK

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Thu Nov 04 00:00:00 EDT 2021

The gammaherpesvirus 68 viral cyclin facilitates expression of LANA.

PLoS pathogens

Niemeyer BF,Sanford B,Gibson JE,Berger JN,Oko LM,Medina E,Clambey ET,van Dyk LF

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Comprehensive mutagenesis identifies the peptide repertoire of a p53 T-cell receptor mimic antibody that displays no toxicity in mice transgenic for human HLA-A*0201.

PloS one

Trenevska I,Anderson AP,Bentley C,Hassanali T,Wiblin S,Maguire S,Pezzella F,Banham AH,Li D

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Wed Sep 29 00:00:00 EDT 2021

Zdhhc2 Is Essential for Plasmacytoid Dendritic Cells Mediated Inflammatory Response in Psoriasis.

Frontiers in immunology

Zhou B,Yang W,Li W,He L,Lu L,Zhang L,Liu Z,Wang Y,Chao T,Huang R,Gu Y,Jia T,Liu Q,Tian S,Pierre P,Maeda T,Liang Y,Kong E

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Wed Jun 23 00:00:00 EDT 2021

CNS Immune Profiling in a Dengue Virus-Infected Immunocompetent Outbred ICR Mice Strain.

Frontiers in cellular and infection microbiology

Shen TJ,Chen CL,Jhan MK,Tseng PC,Lin CF

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Thu Jun 17 00:00:00 EDT 2021

The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cellular & molecular immunology

Huang X,Hao S,Liu J,Huang Y,Liu M,Xiao C,Wang Y,Pei S,Yu T,Xu J,Wang H,Dai D,Su X,Xiao Y

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

γ-Tocotrienol-Loaded Liposomes for Radioprotection from Hematopoietic Side Effects Caused by Radiotherapeutic Drugs.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine

Lee SG,Kalidindi TM,Lou H,Gangangari K,Punzalan B,Bitton A,Lee CJ,Vargas HA,Park S,Bodei L,Kharas MG,Singh VK,Kishore Pillarsetty NV,Larson SM

15-0193 was used in Flow cytometry/Cell sorting to report a novel radioprotector drug, a liposomal formulation of gamma-tocotrienol (GT3), or GT3-Nano for short, to mitigate bone marrow radiation damage during targeted radionuclide therapy (TRT).

Thu Apr 01 00:00:00 EDT 2021

Intravenously usable fully serotype 3 oncolytic adenovirus coding for CD40L as an enabler of dendritic cell therapy.

Oncoimmunology

Zafar S,Parviainen S,Siurala M,Hemminki O,Havunen R,Tähtinen S,Bramante S,Vassilev L,Wang H,Lieber A,Hemmi S,de Gruijl T,Kanerva A,Hemminki A

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver.

Immunity

Remmerie A,Martens L,Thoné T,Castoldi A,Seurinck R,Pavie B,Roels J,Vanneste B,De Prijck S,Vanhockerhout M,Binte Abdul Latib M,Devisscher L,Hoorens A,Bonnardel J,Vandamme N,Kremer A,Borghgraef P,Van Vlierberghe H,Lippens S,Pearce E,Saeys Y,Scott CL

15-0193 was used in Flow cytometry/Cell sorting to highlight considerable heterogeneity within the macrophage pool and suggest a need for more specific macrophage targeting strategies in metabolic-associated fatty liver disease (MAFLD).

Tue Sep 15 00:00:00 EDT 2020

Peritoneal Cells Mediate Immune Responses and Cross-Protection Against Influenza A Virus.

Frontiers in immunology

Gautam A,Park BK,Kim TH,Akauliya M,Kim D,Maharjan S,Park J,Kim J,Lee H,Park MS,Lee Y,Kwon HJ

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Mon Aug 17 00:00:00 EDT 2020

MALT1 targeting suppresses CARD14-induced psoriatic dermatitis in mice.

EMBO reports

Van Nuffel E,Staal J,Baudelet G,Haegman M,Driege Y,Hochepied T,Afonina IS,Beyaert R

15-0193-82 was used in Flow Cytometry to investigate if inducible keratinocyte-specific expression of CARD14E138A in mice rapidly induces epidermal thickening and inflammation as well as increased expression of several genes associated with psoriasis in humans.

Fri Jul 03 00:00:00 EDT 2020

Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection.

Immunity

Bosteels C,Neyt K,Vanheerswynghels M,van Helden MJ,Sichien D,Debeuf N,De Prijck S,Bosteels V,Vandamme N,Martens L,Saeys Y,Louagie E,Lesage M,Williams DL,Tang SC,Mayer JU,Ronchese F,Scott CL,Hammad H,Guilliams M,Lambrecht BN

15-0193 was used in Flow cytometry/Cell sorting to show that monocyte-derived inflammatory DCs’’ are a mixture of monocyte-derived MCs that have little migratory and APC potential and bona fide pre-cDCderived, CD26-expressing inf-cDC2s that depend on Flt3L but not on GM-CSF.

Tue Jun 16 00:00:00 EDT 2020

Excessive Costimulation Leads to Dysfunction of Adoptively Transferred T Cells.

Cancer immunology research

Wijewarnasuriya D,Bebernitz C,Lopez AV,Rafiq S,Brentjens RJ

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Critical Roles of E2F3 in Growth and Musculo-skeletal Phenotype in Mice.

International journal of medical sciences

Kim HR,Rahman FU,Kim KS,Kim EK,Cho SM,Lee K,Moon OS,Seo YW,Yoon WK,Won YS,Kang H,Kim HC,Nam KH

15-0193-82 was used in Flow Cytometry to suggest that E2F3 has a pivotal role in muscle and bone development, and affect normal mouse growth.

Thu Apr 30 00:00:00 EDT 2020

Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus.

Scientific reports

Goldstein JM,Valido A,Lewandowski JP,Walker RG,Mills MJ,Messemer KA,Besseling P,Lee KH,Wattrus SJ,Cho M,Lee RT,Wagers AJ

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Mon Dec 09 00:00:00 EST 2019

Prolonged Deleterious Influences of Chemotherapeutic Agent CPT-11 on Resident Peritoneal Macrophages and B1 Cells.

Frontiers in immunology

Bai WJ,Li CG,Zhang CC,Xu LH,Zeng QZ,Hu B,Hong Z,He XH,Ouyang DY

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Cell-intrinsic depletion of Aml1-ETO-expressing pre-leukemic hematopoietic stem cells by K-Ras activating mutation.

Haematologica

Di Genua C,Norfo R,Rodriguez-Meira A,Wen WX,Drissen R,Booth CAG,Povinelli B,Repapi E,Gray N,Carrelha J,Kettyle LM,Jamieson L,Neo WH,Thongjuea S,Nerlov C,Mead AJ

15-0193 was used in Flow cytometry/Cell sorting to study the mechanism behind absence of KRAS signalling mutations in pre-malignant hematopoietic stem cell compartments.

Fri Nov 01 00:00:00 EDT 2019

NLRX1 inhibits the early stages of CNS inflammation and prevents the onset of spontaneous autoimmunity.

PLoS biology

Gharagozloo M,Mahmoud S,Simard C,Yamamoto K,Bobbala D,Ilangumaran S,Smith MD,Lamontagne A,Jarjoura S,Denault JB,Blais V,Gendron L,Vilariño-Güell C,Sadovnick AD,Ting JP,Calabresi PA,Amrani A,Gris D

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche.

Cell reports

Balzano M,De Grandis M,Vu Manh TP,Chasson L,Bardin F,Farina A,Sergé A,Bidaut G,Charbord P,Hérault L,Bailly AL,Cartier-Michaud A,Boned A,Dalod M,Duprez E,Genever P,Coles M,Bajenoff M,Xerri L,Aurrand-Lions M,Schiff C,Mancini SJC

15-0193 was used in Flow cytometry/Cell sorting to study pro-B cells in their support of stroll cells and their interaction network allowing their retention.

Tue Mar 19 00:00:00 EDT 2019

TET2 Deficiency Causes Germinal Center Hyperplasia, Impairs Plasma Cell Differentiation, and Promotes B-cell Lymphomagenesis.

Cancer discovery

Dominguez PM,Ghamlouch H,Rosikiewicz W,Kumar P,Béguelin W,Fontán L,Rivas MA,Pawlikowska P,Armand M,Mouly E,Torres-Martin M,Doane AS,Calvo Fernandez MT,Durant M,Della-Valle V,Teater M,Cimmino L,Droin N,Tadros S,Motanagh S,Shih AH,Rubin MA,Tam W,Aifantis I,Levine RL,Elemento O,Inghirami G,Green MR,Figueroa ME,Bernard OA,Aoufouchi S,Li S,Shaknovich R,Melnick AM

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

A protocol for generating induced T cells by reprogramming B cells in vivo.

Cell regeneration (London, England)

Weng Q,Hu F,Zhang M,Dong Y,Lv C,Wang Y,Liu X,Wang J

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Mon Mar 19 00:00:00 EDT 2018

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

In Vitro Differentiation of Gata2 and Ly6a Reporter Embryonic Stem Cells Corresponds to In Vivo Waves of Hematopoietic Cell Generation.

Stem cell reports

Kauts ML,Rodriguez-Seoane C,Kaimakis P,Mendes SC,Cortés-Lavaud X,Hill U,Dzierzak E

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Tue Jan 09 00:00:00 EST 2018

Diminished bone regeneration after debridement of posttraumatic osteomyelitis is accompanied by altered cytokine levels, elevated B cell activity, and increased osteoclast activity.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Wagner JM,Jaurich H,Wallner C,Abraham S,Becerikli M,Dadras M,Harati K,Duhan V,Khairnar V,Lehnhardt M,Behr B

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Direct Activation of BAX by BTSA1 Overcomes Apoptosis Resistance in Acute Myeloid Leukemia.

Cancer cell

Reyna DE,Garner TP,Lopez A,Kopp F,Choudhary GS,Sridharan A,Narayanagari SR,Mitchell K,Dong B,Bartholdy BA,Walensky LD,Verma A,Steidl U,Gavathiotis E

15-0193 was used in Flow cytometry/Cell sorting to identify BTSA1, a pharmacologically optimised BAX activator that binds with high affinity and specificity to the N-terminal activation site and induces conformational changes to BAX leading to BAX-mediated apoptosis.

Mon Oct 09 00:00:00 EDT 2017

Alkylator-Induced and Patient-Derived Xenograft Mouse Models of Therapy-Related Myeloid Neoplasms Model Clinical Disease and Suggest the Presence of Multiple Cell Subpopulations with Leukemia Stem Cell Activity.

PloS one

Jonas BA,Johnson C,Gratzinger D,Majeti R

15-0193 was used in Flow cytometry/Cell sorting to report several new t-AML/MDS mouse models that could potentially be used to further define disease pathogenesis and test novel therapeutics.

Wed Jul 26 00:00:00 EDT 2017

A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens.

The Journal of clinical investigation

Chang AY,Dao T,Gejman RS,Jarvis CA,Scott A,Dubrovsky L,Mathias MD,Korontsvit T,Zakhaleva V,Curcio M,Hendrickson RC,Liu C,Scheinberg DA

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Fri Jun 30 00:00:00 EDT 2017

ADAR1 deletion induces NFκB and interferon signaling dependent liver inflammation and fibrosis.

RNA biology

Ben-Shoshan SO,Kagan P,Sultan M,Barabash Z,Dor C,Jacob-Hirsch J,Harmelin A,Pappo O,Marcu-Malina V,Ben-Ari Z,Amariglio N,Rechavi G,Goldstein I,Safran M

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Thu May 04 00:00:00 EDT 2017

Prolactin Rescues Immature B-Cells from Apoptosis Induced by B-Cell Receptor Cross-Linking.

Journal of immunology research

Flores-Fernández R,Blanco-Favela F,Fuentes-Pananá EM,Chávez-Sánchez L,Gorocica-Rosete P,Pizaña-Venegas A,Chávez-Rueda AK

15-0193 was used in Flow cytometry/Cell sorting to determine the effect of prolactin in an in vitro system of B-cell tolerance, showing that it rescues immature B-cells from apoptosis induced by B-cell receptor cross-linking.

Mon Mar 06 00:00:00 EST 2017

Antigen Targeting to Human HLA Class II Molecules Increases Efficacy of DNA Vaccination.

Journal of immunology (Baltimore, Md. : 1950)

Grodeland G,Fredriksen AB,Løset GÅ,Vikse E,Fugger L,Bogen B

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2016

Protein Kinase D2 Protects against Acute Colitis Induced by Dextran Sulfate Sodium in Mice.

Scientific reports

Xiong J,Zhou MF,Wang YD,Chen LP,Xu WF,Wang YD,Deng F,Liu SD

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Fri Sep 23 00:00:00 EDT 2016

CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity.

Nature communications

Jacoby E,Nguyen SM,Fountaine TJ,Welp K,Gryder B,Qin H,Yang Y,Chien CD,Seif AE,Lei H,Song YK,Khan J,Lee DW,Mackall CL,Gardner RA,Jensen MC,Shern JF,Fry TJ

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Wed Jul 27 00:00:00 EDT 2016

Aging of mice is associated with p16(Ink4a)- and β-galactosidase-positive macrophage accumulation that can be induced in young mice by senescent cells.

Aging

Hall BM,Balan V,Gleiberman AS,Strom E,Krasnov P,Virtuoso LP,Rydkina E,Vujcic S,Balan K,Gitlin I,Leonova K,Polinsky A,Chernova OB,Gudkov AV

15-0193 was used in Flow cytometry/Cell sorting to investigate the role of p16(Ink4a)/β-gal(pH6)-positive macrophages in aging, which previously was attributed solely to senescent cells.

Fri Jul 01 00:00:00 EDT 2016

Medial HOXA genes demarcate haematopoietic stem cell fate during human development.

Nature cell biology

Dou DR,Calvanese V,Sierra MI,Nguyen AT,Minasian A,Saarikoski P,Sasidharan R,Ramirez CM,Zack JA,Crooks GM,Galic Z,Mikkola HK

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

IL35-Producing B Cells Promote the Development of Pancreatic Neoplasia.

Cancer discovery

Pylayeva-Gupta Y,Das S,Handler JS,Hajdu CH,Coffre M,Koralov SB,Bar-Sagi D

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Novel CXCL13 transgenic mouse: inflammation drives pathogenic effect of CXCL13 in experimental myasthenia gravis.

Oncotarget

Weiss JM,Robinet M,Aricha R,Cufi P,Villeret B,Lantner F,Shachar I,Fuchs S,Souroujon MC,Berrih-Aknin S,Le Panse R

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2016

Ten Weeks of Infection with a Tissue-Invasive Helminth Protects against Local Immune Complex-Mediated Inflammation, but Not Cutaneous Type I Hypersensitivity, in Previously Sensitized Mice.

Journal of immunology (Baltimore, Md. : 1950)

Evans H,Killoran KE,Mitre BK,Morris CP,Kim SY,Mitre E

15-0193 was used in Flow cytometry/Cell sorting to demonstrate that chronic filarial infection suppresses eosinophilic responses to antibody-mediated activation.

Thu Oct 01 00:00:00 EDT 2015

dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.

Nature communications

Lim S,Kim WJ,Kim YH,Lee S,Koo JH,Lee JA,Yoon H,Kim DH,Park HJ,Kim HM,Lee HG,Yun Kim J,Lee JU,Hun Shin J,Kyun Kim L,Doh J,Kim H,Lee SK,Bothwell ALM,Suh M,Choi JM

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Tue Sep 15 00:00:00 EDT 2015

Cis-regulatory mechanisms governing stem and progenitor cell transitions.

Science advances

Johnson KD,Kong G,Gao X,Chang YI,Hewitt KJ,Sanalkumar R,Prathibha R,Ranheim EA,Dewey CN,Zhang J,Bresnick EH

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

B cell Rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses.

Journal of immunology (Baltimore, Md. : 1950)

Pone EJ,Lam T,Lou Z,Wang R,Chen Y,Liu D,Edinger AL,Xu Z,Casali P

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Toxoplasma gondii superinfection and virulence during secondary infection correlate with the exact ROP5/ROP18 allelic combination.

mBio

Jensen KD,Camejo A,Melo MB,Cordeiro C,Julien L,Grotenbreg GM,Frickel EM,Ploegh HL,Young L,Saeij JP

15-0193 was used in Flow cytometry/Cell sorting to investigate the interaction between infected host and Toxoplasma gondii strains in endemic regions, showing that secondary infection correlates with the ROP5/ROP18 allele combinations.

Tue Feb 24 00:00:00 EST 2015

Renal F4/80+ CD11c+ mononuclear phagocytes display phenotypic and functional characteristics of macrophages in health and in adriamycin nephropathy.

Journal of the American Society of Nephrology : JASN

Cao Q,Wang Y,Wang XM,Lu J,Lee VW,Ye Q,Nguyen H,Zheng G,Zhao Y,Alexander SI,Harris DC

15-0193 was used in Flow cytometry/Cell sorting to identify the phenotype and function of renal mononuclear phagocytes expressing key markers of both macrophages and dendritic cells.

Sun Feb 01 00:00:00 EST 2015

Role of caveolin-3 in lymphocyte activation.

Life sciences

Tran C,Stary CM,Schilling JM,Bentley B,Patel HH,Roth DM

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Thu Jan 15 00:00:00 EST 2015

DAP12 overexpression induces osteopenia and impaired early hematopoiesis.

PloS one

Despars G,Pandruvada SN,Anginot A,Domenget C,Jurdic P,Mazzorana M

15-0193 was used in Flow cytometry/Cell sorting to analyse the bone phenotype of mice overexpressing the human ITAM-bearing transmembrane signalling adaptor DAP12.

Thu Jan 16 00:00:00 EST 2014

Cytometric profiling in various clinical forms of multiple sclerosis with respect to CD21+, CD32+, and CD35+ B and T cells.

Translational neurodegeneration

Zandieh A,Izad M,Fakhri M,Amirifard H,Khazaeipour Z,Harirchian MH

15-0193 was used in Flow cytometry/Cell sorting to evaluate the frequency of various immune cell subsets expressing certain CD cell surface markers in MS clinical courses.

Tue Jul 02 00:00:00 EDT 2013

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

CD11c+ cells are required for antigen-induced increase of mast cells in the lung.

Journal of immunology (Baltimore, Md. : 1950)

Dahlin JS,Feinstein R,Cui Y,Heyman B,Hallgren J

15-0193 was used in Flow cytometry/Cell sorting to determine the contribution of Ag-stimulated CD11c(+) cells to the allergic response in asthmatics.

Mon Oct 15 00:00:00 EDT 2012

Host differences in influenza-specific CD4 T cell and B cell responses are modulated by viral strain and route of immunization.

PloS one

Sundararajan A,Huan L,Richards KA,Marcelin G,Alam S,Joo H,Yang H,Webby RJ,Topham DJ,Sant AJ,Sangster MY

15-0193 was used in Flow cytometry/Cell sorting to identify inherent host influences on characteristics of influenza-specific CD4 T cell and B cell responses.

Fri Aug 03 00:00:00 EDT 2012

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

Published figure using CD19 monoclonal antibody (Product # 15-0193-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Dermatan sulfate interacts with dead cells and regulates CD5(+) B-cell fate: implications for a key role in autoimmunity.

The American journal of pathology

Wang JY,Lee J,Yan M,Rho JH,Roehrl MH

15-0193 was used in Flow cytometry/Cell sorting to investigate the mechanisms underlying the positive selection and expansion of CD5(+) B cells, showing that dermatan sulfate interacts with dead cells and regulates CD5(+) B-cell fate.