Staining of normal human peripheral blood cells with Anti-Human CD16 PE (Product # 12-0168) and Mouse IgG2b K Isotype Control APC (Product # 17-4732) (left) or Anti-Human CD193 (CCR3) APC (right). Cells in the large scatter population were used for analysis.
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2b, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5 µL (0.25 µg)/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The eBio5E8-G9-B4 monoclonal antibody reacts with human CD193 (CCR3, CKR3). CD193 is a member of the seven transmembrane G-protein coupled receptor (GPCR) family, and is a high affinity chemokine receptor for the chemokines eotaxin-1 (CCL11), eotaxin-2 (CCL24), eotaxin-3 (CCL26) and MCP-4 (CCL13), but has also been reported to bind RANTES, MCP-3 and MCP-4. CD193 is highly expressed on the surface of eosinophils, and is the key eosinophil chemokine receptor responsible for the regulation of eosinophil migration and function. In addition to eosinophils, CD193 is also expressed on CD4+ TH2 cells, basophils, mast cells, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitors and airway epithelial cells. CD193 likely plays an important role in the migration of eosinophils in allergic airway inflammation and asthma. CD193 can also serve as a receptor for HIV-1 cell entry.
Applications Reported: This eBio5E8-G9-B4 (5E8-G9-B4) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio5E8-G9-B4 (5E8-G9-B4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors. The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines. One of the CCR receptors, CCR3 (eotaxin receptor), is expressed on eosinophils and certain T cell population respond to a variety of CC chemokines apart from eotaxin, including RANTES, monocyte chemotactic protein (MCP)-2, MCP-3, and MCP-4. CCR3 facilitated infection by a more restricted subset of primary viruses, and binding of the CCR3 ligand, eotaxin, and inhibited infection by these isolates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells.||Sallusto F,Mackay CR,Lanzavecchia A||Science (New York, N.Y.) (277:2005)||1997|
|Not Applicable||Not Cited||Eotaxin-2, a novel CC chemokine that is selective for the chemokine receptor CCR3, and acts like eotaxin on human eosinophil and basophil leukocytes.||Forssmann U,Uguccioni M,Loetscher P,Dahinden CA,Langen H,Thelen M,Baggiolini M||The Journal of experimental medicine (185:2171)||1997|
|Not Applicable||Not Cited||Chemokine receptor usage by human eosinophils. The importance of CCR3 demonstrated using an antagonistic monoclonal antibody.||Heath H,Qin S,Rao P,Wu L,LaRosa G,Kassam N,Ponath PD,Mackay CR||The Journal of clinical investigation (99:178)||1997|
|Not Applicable||Not Cited||The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates.||Choe H,Farzan M,Sun Y,Sullivan N,Rollins B,Ponath PD,Wu L,Mackay CR,LaRosa G,Newman W,Gerard N,Gerard C,Sodroski J||Cell (85:1135)||1996|