|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Activated rat T helper cells.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/50|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
We recommend the use of fixation for optimal staining results. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Morover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations.
MA1-81578 was used in flow cytometry to investigate the expression of prolactin receptor in lymphocyte subsets
|Gagnerault MC,Touraine P,Savino W,Kelly PA,Dardenne M||Journal of immunology (Baltimore, Md. : 1950) (150:5673)||1993|