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Western blot analysis was performed on whole cell extracts (30 ug lysate) of Ramos (Lane1), A-431 (Lane2), K562 (Lane 3) and Raji (Lane4). The blots were probed with Anti-CD2 Mouse Monoclonal Antibody (Product# MA0200, 0.5-2 ug/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 626520, 1:4000 dilution). A 40 kDa band corresponding to CD2 was observed across cell lines tested expect for K562. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0341BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product# SLF2000S). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human LFA-2 (CD2)|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Inhibition Assays (IA)||0.5 ug/test|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA0200 targets CD2 in FACS, IA, IP, and WB applications and shows reactivity with Human samples. This antibody does not react with rat tissue in Western blot applications.
The MA0200 immunogen is human LFA-2 (CD2).
MA0200 detects CD2 which has a predicted molecular weight of approximately 37 kDa.
CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells (summarized by Sewell et al., 1986 [PubMed 3490670]). It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Human||1 ug/10x5 cells||
Antibody blocking of MHC II on human activated regulatory T cells abrogates their suppressive potential.
MA0200 was used in blocking/activating experiment to investigate the role of MHC II in the regulation of Treg cell function
|Peiser M,Becht A,Wanner R||Allergy (62:773)||2007|
Mechanisms of Cellular Avidity Regulation in CD2-CD58-Mediated T Cell Adhesion.
MA0200 was used in blocking/activating experiment to study the CD2-CD58-mediated T cell adhesion process
|Zhu DM,Dustin ML,Cairo CW,Thatte HS,Golan DE||ACS chemical biology (1:649)||2006|
Selective deletion of antigen-specific, activated T cells by a humanized MAB to CD2 (MEDI-507) is mediated by NK cells.
MA0200 was used in blocking/activating experiment to investigate the role of NK cells during selective deletion of antigen-specific, activated T cells by a humanized MAB to CD2
|Branco L,Barren P,Mao SY,Pfarr D,Kaplan R,Postema C,Langermann S,Koenig S,Johnson S||Transplantation (68:1588)||1999|
Intercellular adhesion molecule-1 and leukocyte function-associated antigen-3 provide costimulation for superantigen-induced T lymphocyte proliferation in the absence of a specific presenting molecule.
MA0200 was used in blocking/activating experiment to show the role of LFA-3/CD2 interactions in the T cell activation
|Lamphear JG,Stevens KR,Rich RR||Journal of immunology (Baltimore, Md. : 1950) (160:615)||1998|
CD28-mediated up-regulation of beta 1-integrin adhesion involves phosphatidylinositol 3-kinase.
MA0200 was used in flow cytometry to investigate the role of PI3K in beta 1-integrin adhesion induced by CD28
|Zell T,Hunt SW,Mobley JL,Finkelstein LD,Shimizu Y||Journal of immunology (Baltimore, Md. : 1950) (156:883)||1996|