|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.25 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The AISB12 monoclonal antibody reacts with mouse CD20. CD20 is a B cell-specific 32kDa membrane protein involved in intracellular calcium regulation. CD20 expression begins after the induction of CD19 during development, and is coexpressed with IgM during the pre-B to immature B cell transition in bone marrow. Expression then increases during maturation with almost all mature cells expressing some level of CD20. The human CD20 antigen has proven to be an effective immunotherapeutic target in B cell lymphoma and several autoimmune diseases including rheumatoid arthritis and type I diabetes. CD20 consists of 4 transmembrane domains and a 44 amino acid extracellular loop domain where AISB12 binds.
Applications Reported: This AISB12 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This AISB12 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Active vaccination with Dickkopf-1 induces protective and therapeutic antitumor immunity in murine multiple myeloma.||Qian J,Zheng Y,Zheng C,Wang L,Qin H,Hong S,Li H,Lu Y,He J,Yang J,Neelapu S,Kwak LW,Hou J,Yi Q||Blood (119:161)||2012|
|Not Applicable||Not Cited||The innate mononuclear phagocyte network depletes B lymphocytes through Fc receptor-dependent mechanisms during anti-CD20 antibody immunotherapy.||Uchida J,Hamaguchi Y,Oliver JA,Ravetch JV,Poe JC,Haas KM,Tedder TF||The Journal of experimental medicine (199:1659)||2004|
|Not Applicable||Not Cited||Mouse CD20 expression and function.||Uchida J,Lee Y,Hasegawa M,Liang Y,Bradney A,Oliver JA,Bowen K,Steeber DA,Haas KM,Poe JC,Tedder TF||International immunology (16:119)||2004|
|Not Applicable||Not Cited||Cloning of a complementary DNA encoding a new mouse B lymphocyte differentiation antigen, homologous to the human B1 (CD20) antigen, and localization of the gene to chromosome 19.||Tedder TF,Klejman G,Disteche CM,Adler DA,Schlossman SF,Saito H||Journal of immunology (Baltimore, Md. : 1950) (141:4388)||1988|