Description: The TEK4 monoclonal antibody reacts with mouse Tie-2, also known as CD202. A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers^-, c-Kit^+, Sca1^+ cells. Long-term multilineage repopulating cells were detected in Tie-2^+, Lineage^-, c-Kit^+, Sca1^+ cells but not in Tie-2^-, Lineage^-, c-Kit^+, Sca1^+ cells.
Applications Reported: This TEK4 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.
Applications Tested: The TEK4 antibody has been tested by blocking of staining with fluorochrome conjugated TEK4 on mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Angiopoietin-1 receptor; CD202B; Endothelial tyrosine kinase; HYK; mTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; STK1; TIE-2; TIE2; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; tyrosine-protei; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; VMCM; VMCM1
Gene Aliases: AA517024; Cd202b; Hyk; STK1; Tek; Tie-2; Tie2
UniProt ID: (Mouse) Q02858
Entrez Gene ID: (Mouse) 21687